Inhibitory effect of theophylline on repair of potentially lethal MMS-induced damages to DNA in V79 cells

Slameñová, D; Bohušová, T.; Oravec, C

doi:10.1016/0165-1218(90)90038-4

Cited by 11 publications

(2 citation statements)

References 20 publications

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“…By the study of inhibition of daughter DNA synthesis, theophylline was shown to be a metabolic inhibitor of DNA. It was found that theophylline interfered with post-replicative repair (Lehmann and Kirk-Bell, 1974;Bernheim and Falk, 1983;Slamenova et al, 1990). Mutagenicity of theophylline was not demonstrated in dominant lethal test in mice (Timson, 1975).…”

Section: Introductionmentioning

confidence: 99%

Study of the biological effects of theophylline on V79 cells: Viability, membrane permeability, and metabolic cooperation

1991

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The effect of theophylline, a specific inhibitor of phosphodiesterase, on gap junction-mediated intercellular communication between Chinese hamster V79 cells was examined. It was found that addition of theophylline to coculture of 6-thioguanine-resistant (TGr) and 6-thioguanine-sensitive (TGs) V79 cells significantly increased the recovery of TGr cells. This finding indicates an inhibition of metabolic cooperation of V79 cells by theophylline. Theophylline was tested at concentrations less than 0.3 mg/ml, which were neither cytotoxic (after short or continuous exposure) nor inhibited the synthesis of DNA, RNA, and proteins. At the tested concentrations, no change was found in the membrane permeability of cells. Theophylline did not increase the incorporation of glucose into the cells.

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Section: Introductionmentioning

confidence: 99%

Study of the biological effects of theophylline on V79 cells: Viability, membrane permeability, and metabolic cooperation

1991

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“…Так, теофиллин подавляет репарацию 1 ДНК. И если по фону его применения накладывается действие мутагенов (проверялся в эксперименте метилметансульфонат), то повреждающие эффекты последних резко усиливаются [74]. Данных о таких эффектах накопилось уже достаточно много.…”

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The problem of survival: from transitory stay in extreme situation to constant inhabiting in extreme conditions

Kordyum

1992

Biopolym. Cell

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Decemtione (imidan)‐induced single‐strand breaks to human dna, mutations at the hgprt locus of v79 cells, and morphological transformations of embryo cells

Slameñová

Dušinská

Gábelová

et al. 1992

Environ and Mol Mutagen

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To study the genotoxic activity of Decemtione (Imidan), this substance was subjected to a series of tests. After preliminary cytotoxicity testing, the capacity of Decemtione to damage human DNA was determined by alkaline elution of DNA and DNA unwinding. Both tests gave positive results, suggesting that Decemtione was able to induce single-strand breaks in DNA. This capacity was higher in the absence and lower in the presence of the S9 fraction. The potential mutagenicity of Decemtione was followed on the basis of its ability to induce resistance to 6-thioguanine in V79 hamster cells. Unlike the induction of single-strand breaks, Decemtione showed, in the absence of the metabolic activation system, a very weak mutagenic effect, which was, however, significantly higher in the presence of the S9 fraction. The ability of the substance to transform diploid cells under in vitro conditions was followed on the basis of morphological transformation of Syrian hamster embryo cells. The results showed that Decemtione, like positive carcinogenes, induced a significant elevation in morphologically transformed colonies of embryo cells. The results suggest a carcinogenic potential of this organophosphate insecticide.

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Inhibitory effect of theophylline on repair of potentially lethal MMS-induced damages to DNA in V79 cells

Cited by 11 publications

References 20 publications

Study of the biological effects of theophylline on V79 cells: Viability, membrane permeability, and metabolic cooperation

Study of the biological effects of theophylline on V79 cells: Viability, membrane permeability, and metabolic cooperation

The problem of survival: from transitory stay in extreme situation to constant inhabiting in extreme conditions

Decemtione (imidan)‐induced single‐strand breaks to human dna, mutations at the hgprt locus of v79 cells, and morphological transformations of embryo cells

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