Inhibitory effect of the Larix sibirica and its various flavonoids on the IgE-stimulated mast cell activation and anaphylaxis

Kim, Myungsuk; Kim, Sun Young; Randy, Ahmad; Lim, Sue Ji; Dorjsembe, Banzragch; Nho, Chu Won

doi:10.1016/j.jff.2016.10.009

Cited by 3 publications

(3 citation statements)

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“…The degranulation components including allergenic mediators and cytokines secreted by cells are important criteria in expressing the food allergy response [38]. Furthermore, as markers of the degranulation in mast cells, β-hexosaminidase and histamine play a crucial part in allergic diseases [23]. Our experiments confirm that SBF, PSBF, and their active compounds do indeed suppress the degranulation of activated mast cells via reducing the release of β-hexosaminidase and histamine, which is consistent with previous results of Itoh et al [39] and Beáta et al [40], who indicate that kaempferol, quercitrin, quercetin, other flavonoids, and natural plant extracts, as well as abundant plant components in fruits and vegetables, likely have antiallergic properties.…”

Section: Discussionmentioning

confidence: 99%

“…Western blotting was performed as described previously with some modifications [23]. All proteins were isolated from RBL-2H3 cells in various groups, whose protein concentrations were determined using the BCA Protein Assay Kit.…”

Section: Western Boltmentioning

confidence: 99%

“…Firstly, the effects of SBF, PSBF, and five compounds on degranulation was assessed in RBL-2H3 cells sensitized by IgE. β-hexosaminidase and histamine, as main allergic mediators, were released by granulated mast cells stimulated with IgE [23]. Quercitrin at 40 µmol/L suppressed β-hexosaminidase release to a greater degree than quercetin, isorhamnetin, and kaempferol (Figure 4A-E).…”

Section: Identification and Quantified Of Sbf And Psbfmentioning

confidence: 99%

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Sea Buckthorn Flavonoid Extracted by High Hydrostatic Pressure Inhibited IgE-Stimulated Mast Cell Activation through the Mitogen-Activated Protein Kinase Signaling Pathway

Yan,

Feng,

et al. 2024

Foods

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Sea buckthorn (Hippophaë rhamnoides L.), as one of the Elaeagnaceae family, has the significant function of anti-tumor, anti-inflammation, anti-oxidation, and other physiological activities. High hydrostatic pressure (HHP) extraction has the advantages of being easy and efficient, while maintaining biological activity. In this study, sea buckthorn flavonoid (SBF) was extracted with HHP and purified sea buckthorn flavonoid (PSBF) was isolated by AB-8 macroporous resin column. HPLC analysis was used to quantified them. In addition, the effect of anti-allergy in RBL-2H3 cells by SBF, PSBF, and their flavonoid compounds was evaluated. The results demonstrate the conditions for obtaining the maximum flavonoid amount of SBF: 415 MPa for 10 min, 72% ethanol concentration, and a liquid to solid ratio of 40 mL/g, which increased the purity from 1.46% to 13.26%. Both SBF and PSBF included rutin, quercitrin, quercetin, isorhamnetin, and kaempferol. In addition, quercitrin, kaempferol, and SBF could regulate Th1/Th2 cytokine balance. Moreover, extracellular Ca2+ influx was reduced by quercitrin and PSBF. Furthermore, rutin, quercetin, iso-rhamnetin, and SBF could also inhibit P-p38 and P-JNK expression, thereby suppressing the phosphorylation of the MAPK signaling pathways. Overall, SBF is effective for relieving food allergy and might be a promising anti-allergic therapeutic agent.

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Section: Discussionmentioning

confidence: 99%

Section: Western Boltmentioning

confidence: 99%

Section: Identification and Quantified Of Sbf And Psbfmentioning

confidence: 99%

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Sea Buckthorn Flavonoid Extracted by High Hydrostatic Pressure Inhibited IgE-Stimulated Mast Cell Activation through the Mitogen-Activated Protein Kinase Signaling Pathway

Yan,

Feng,

et al. 2024

Foods

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Flavonoids as detoxifying and pro-survival agents: What's new?

Bjørklund

Dadar

Chirumbolo

et al. 2017

Food and Chemical Toxicology

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Antiallergic Effect of the Alpha‐Cyclodextrin Moringin Complex in Rat Basophilic Leukaemia (RBL‐2H3) Cell Line

Yousef A. Alnakeeb,

Abdull Razis,

Wei Chan

et al. 2024

Advances in Pharmacological and Pharmaceutical Sciences

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Allergic diseases (ADs) are a major concern when it comes to public well‐being. Moringa oleifera Lam is a tropical plant that is used in traditional medicine due to the presence of isothiocyanate. The present study investigated the antiallergic properties of 4‐(α‐L‐rhamnopyranosyloxy)‐benzyl isothiocyanate or moringin isolated from Moringa oleifera seeds in the form of alpha‐cyclodextrin‐moringin (α‐CD/MG) complex on rat basophilic leukaemia (RBL‐2H3) cell line at both the early and late stages of an allergic reaction. The α‐CD/MG complex was initially elucidated using nuclear magnetic resonance (NMR) followed by the 3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium inner salt proliferation assay to evaluate the cytotoxicity and cell viability with respect to ketotifen fumarate (KF) and α‐CD/MG. The release of beta‐hexosaminidase (β‐hexosaminidase) and histamine was used to determine the level of inhibition in the early stage while the suppression of the release of prostaglandin (PGD2), tumour necrosis factor‐alpha (TNF‐α), and interleukin (IL‐4) was considered in the late stage. Higher concentrations of α‐CD/MG (5 μM, p < 0.001) in mast cell degranulation significantly inhibited the expression of β‐hexosaminidase, histamine, TNF‐α, PGD2, and IL‐4 in both the early and late stages. Thus, α‐CD/MG can potentially be developed as an antiallergic drug as it has the ability to inhibit allergic responses in the late and early stages.

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Inhibitory effect of the Larix sibirica and its various flavonoids on the IgE-stimulated mast cell activation and anaphylaxis

Cited by 3 publications

References 44 publications

Sea Buckthorn Flavonoid Extracted by High Hydrostatic Pressure Inhibited IgE-Stimulated Mast Cell Activation through the Mitogen-Activated Protein Kinase Signaling Pathway

Sea Buckthorn Flavonoid Extracted by High Hydrostatic Pressure Inhibited IgE-Stimulated Mast Cell Activation through the Mitogen-Activated Protein Kinase Signaling Pathway

Flavonoids as detoxifying and pro-survival agents: What's new?

Antiallergic Effect of the Alpha‐Cyclodextrin Moringin Complex in Rat Basophilic Leukaemia (RBL‐2H3) Cell Line

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