Inhibitory effect of Thai propolis on human osteoclastogenesis

Wimolsantirungsri, Nattaporn; Makeudom, Anupong; Louwakul, Phumisak; Sastraruji, Thanapat; Chailertvanitkul, Pattama; Supanchart, Chayarop; Krisanaprakornkit, Suttichai

doi:10.1111/edt.12401

Cited by 8 publications

(13 citation statements)

References 32 publications

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“…Some substances tested to treat the root surface before delayed tooth replantation have efficacy as osteoporotic therapeutic agents that inhibit osteoclast activity and modulate immune inflammatory reactions. 6,7,8 These substances include Emdogain, alendronate, odanacatib 6 and propolis. 7,8 In recent years, compounds of natural origin, obtained from plants such as curcumin and piperine, have been tested to formulate anti-inflammatory and antiosteoclastogenic drugs having the potential to treat and prevent replacement resorption of replanted teeth.…”

Section: Introductionmentioning

confidence: 99%

“…6,7,8 These substances include Emdogain, alendronate, odanacatib 6 and propolis. 7,8 In recent years, compounds of natural origin, obtained from plants such as curcumin and piperine, have been tested to formulate anti-inflammatory and antiosteoclastogenic drugs having the potential to treat and prevent replacement resorption of replanted teeth. 9 In this respect, a recent study tested the effect of a plant lectin on stimulated gingival fibroblasts, with promising results 10 for future use as a possible therapy for delayed replanted teeth.…”

Section: Introductionmentioning

confidence: 99%

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Effect of ScLL and 15d-PGJ2 on viability and cytokine release in LPS-stimulated fibroblasts: an in vitro study

et al. 2020

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This study evaluated the effect of a cyclopentenone-type PG, 15-Deoxy-Δ12,14 -PG J2 (15d-PGJ 2 ), and lectin (ScLL) on the viability of human gingival fibroblasts (HGFs), and on IL-6 and TGFβ-1 release by these fibroblasts, stimulated with lipopolysaccharide (LPS). HGFs were stimulated with LPS 10 μg/ml and treated with 15d-PGJ 2 1 and 2 μg/ml, and ScLL 2 and 5 μg/ml, for 1 and 3h, and then evaluated for viability by MTT assay. Supernatant was collected to detect IL-6 and TGFβ-1 release, by ELISA. Positive control was cells kept in Dulbecco's Modified Eagle's Medium, and negative control was those kept in LPS. Data were analyzed by ANOVA and Dunnett's test (α = 0.05). No significant difference was found in viability among experimental groups at 1h (p > 0.05). Percentage of ScLL 5 µg/ml viable cells was similar to that of positive control at evaluated periods (p > 0.05), whereas the other groups had lower levels than the positive control (p < 0.05). IL-6 release was statistically higher for ScLL 5 μg/ml and 15d-PGJ 2 2 µg/ml at 1h, compared with the other treated groups and positive control (p < 0.05). No significant differences were found among the groups at 3h (p > 0.05), except for ScLL 2 µg/ml and 15d-PGJ 2 1 µg/ml, which showed lower IL-6 release compared with that of negative control (p < 0.05). No significant difference was found among the groups for TGFβ-1 release (p > 0.05). Results indicated that ScLL 5 μg/ml did not interfere in viability, and ScLL 2 µg/ml and 15d-PGJ 2 1 µg/ml demonstrated reduced IL-6 release. Tested substances had no effect on TGFβ-1 release.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of ScLL and 15d-PGJ2 on viability and cytokine release in LPS-stimulated fibroblasts: an in vitro study

et al. 2020

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“…It is possible that disruption of periostin‐mediated homeostatic relationship within fibrous connective tissues of the PDL might lead to a deteriorated biomechanical properties of the PDL, providing that periostin is a key element required for collagen I fibrillogenesis in variety of connective tissues 28 . Wimolsantirungsri et al showed that Thai propolis extract can inhibit human osteoclast formation and function, 29 which may be beneficial for prevention of root resorption following replantation of avulsed teeth. Collectively, these findings might suggest that Thai propolis extract when used in moderation as a storage medium enables PDL cells to preserve their characteristics of periostin mRNA expression for up to 12 hours extra‐oral dry time.…”

Section: Discussionmentioning

confidence: 99%

Preservation of the viability and gene expression of human periodontal ligament cells by Thai propolis extract

Bunwanna

Damrongrungruang

Puasiri

et al. 2020

Dental Traumatology

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Background/Aim: Success of tooth replantation depends on the quality and quantity of periodontal ligament (PDL) cells. The aims of this study were to evaluate Thai propolis extract as a storage medium for maintaining PDL cell viability and preserving gene expressions in PDL tissues. Materials and Methods: PDL cells from human premolars were tested for cytotoxicity of the extract by PrestoBlue assay to determine a non-toxic concentration. Subsequently, 96 freshly extracted premolars were allocated into different treatment groups. Control groups were freshly extracted premolars or they had been stored dry for 12 hours. Experimental avulsed teeth were created by leaving them air-dried for 30 minutes immediately after extraction, then they were immersed in Thai propolis extract, HBSS or milk for 3, 6 and 12 hours. After tooth storage, the remaining PDL cells were determined for their cell viability. RNA isolated from PDL tissues of three premolars treated similarly was analysed for periostin and S100A4 expressions using RT-qPCR. Results: Thai propolis extract at 0.625 mg mL −1 promoted the greatest PDL cell viability. Tooth storage in 0.625 mg mL −1 Thai propolis extract, HBSS or milk showed no difference in maintaining cell viability. Periostin mRNA level was preserved by Thai propolis extract. Expression of S100A4 mRNA in PDL tissues stored in all tested media was dampened. Conclusions: PDL cells from mock avulsed teeth stored in 0.625 mg mL −1 Thai propolis extract for 3, 6 and 12 hours remained viable and the expression of periostin was preserved. This study suggests this extract as an alternative for a tooth storage medium for up to 12 hours. However, transporting an avulsed tooth in a storage medium for extended extra-oral time might affect the PDL cell phenotypes.

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“…For in vitro osteoclastogenesis studies, mouse marrow cells, human peripheral blood mononuclear cells, and RAW264.7 cell line were the cell models used. Mouse marrow and RAW264.7 cell were stimulated with receptor activator of NF-kB (RANK) ligand (RANKL) and cultured with 1-10 µL of propolis [39], while human peripheral blood mononuclear cells were stimu-lated with macrophage colony-stimulating factor (M-CSF) and RANKL and cultured with 0.025-10 mg/mL of propolis [40]. For osteoblastogenesis studies, the cell models used were MC3T3-E1 and MG-63 cell lines.…”

Section: Study Characteristicsmentioning

confidence: 99%

“…Overall, 2 studies showed that propolis suppressed osteoclastogenesis [39,40], while 2 studies showed that propolis promoted osteoblastogenesis [41,42]. In the animal experiments, propolis inhibited bone loss due to periodontitis in 2 studies [48,49], bone loss due to periodontitis/STZ-induced diabetes in 1 study [56], bone loss due to OTM in 3 studies [50][51][52] and bone loss due to dental implantitis in 1 study [41].…”

Section: Study Characteristicsmentioning

confidence: 99%

Application of Propolis in Protecting Skeletal and Periodontal Health—A Systematic Review

Ekeuku

Chin

2021

Molecules

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Chronic inflammation and oxidative stress are two major mechanisms leading to the imbalance between bone resorption and bone formation rate, and subsequently, bone loss. Thus, functional foods and dietary compounds with antioxidant and anti-inflammatory could protect skeletal health. This review aims to examine the current evidence on the skeletal protective effects of propolis, a resin produced by bees, known to possess antioxidant and anti-inflammatory activities. A literature search was performed using Pubmed, Scopus, and Web of Science to identify studies on the effects of propolis on bone health. The search string used was (i) propolis AND (ii) (bone OR osteoporosis OR osteoblasts OR osteoclasts OR osteocytes). Eighteen studies were included in the current review. The available experimental studies demonstrated that propolis could prevent bone loss due to periodontitis, dental implantitis, and diabetes in animals. Combined with synthetic and natural grafts, it could also promote fracture healing. Propolis protects bone health by inhibiting osteoclastogenesis and promoting osteoblastogenesis, partly through its antioxidant and anti-inflammatory actions. Despite the promising preclinical results, the skeletal protective effects of propolis are yet to be proven in human studies. This research gap should be bridged before nutraceuticals based on propolis with specific health claims can be developed.

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Inhibitory effect of Thai propolis on human osteoclastogenesis

Abstract: Thai propolis can inhibit human osteoclast formation and function, which may be beneficial for prevention of root resorption following replantation of avulsed teeth.

Cited by 8 publications

References 32 publications

Effect of ScLL and 15d-PGJ2 on viability and cytokine release in LPS-stimulated fibroblasts: an in vitro study

Effect of ScLL and 15d-PGJ2 on viability and cytokine release in LPS-stimulated fibroblasts: an in vitro study

Preservation of the viability and gene expression of human periodontal ligament cells by Thai propolis extract

Application of Propolis in Protecting Skeletal and Periodontal Health—A Systematic Review

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