Inhibitory effect of superoxide-generating quinones on superoxide dismutase

Smith, Martyn T.; Evans, Celia G.

doi:10.1016/0006-2952(84)90619-1

Cited by 24 publications

(2 citation statements)

References 4 publications

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“…During the initial phase of infection by Leishmania , superoxide is produced as part of the oxidative burst of macrophages in response to phagocytosis [ 6 , 8 ]. The second oxidant produced by macrophages is nitric oxide, which, in contrast to superoxide, is generated after activation of macrophages by IFN-y and TNF [ 9 ], [ 10 ]. Nitric oxide is derived from the oxidation of the terminal guanidine nitrogen of L-arginine by an NADPH-dependent enzyme, NO synthase.…”

Section: Introductionmentioning

confidence: 99%

The Role of Nitric Oxide and Reactive Oxygen Species in the Killing of Leishmania braziliensis by Monocytes from Patients with Cutaneous Leishmaniasis

et al. 2016

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Human cutaneous leishmaniasis (CL) caused by Leishmania braziliensis, presents an exaggerated Th1 response that is associated with ulcer development. Macrophages are the primary cells infected by Leishmania parasites and both reactive oxygen species (ROS) and nitric oxide (NO) are important in the control of Leishmania by these cells. The mechanism involved in the killing of L. braziliensis is not well established. In this study, we evaluate the role of ROS and NO in the control of L. braziliensis infection by monocytes from CL patients. After in vitro infection with L. braziliensis, the oxidative burst by monocytes from CL patients was higher when compared to monocytes from healthy subjects (HS). Inhibition of the ROS pathway caused a significant decrease in the oxidative burst in L. braziliensis infected monocytes from both groups. In addition, we evaluated the intracellular expression of ROS and NO in L. braziliensis-infected monocytes. Monocytes from CL patients presented high expression of ROS after infection with L. braziliensis. The expression of NO was higher in monocytes from CL patients as compared to expression in monocytes from HS. A strong positive correlation between NO production and lesion size of CL patients was observed. The inhibition of ROS production in leishmania-infected monocytes from CL patients allowed the growth of viable promastigotes in culture supernatants. Thus, we demonstrate that while production of ROS is involved in L. braziliensis killing, NO alone is not sufficient to control infection and may contribute to the tissue damage observed in human CL.

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Section: Introductionmentioning

confidence: 99%

The Role of Nitric Oxide and Reactive Oxygen Species in the Killing of Leishmania braziliensis by Monocytes from Patients with Cutaneous Leishmaniasis

et al. 2016

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“…A las células, a las que se les retiró el medio y se lavó con PBS estéril, se les añadió la mezcla con y sin superóxido dismutasa (SOD) a una concentración de 10 mg/ml con el fin excluir la reducción del citocromo C no evitable por SOD [47]. 5 minutos antes de ser estimuladas las células, se añadió citocalasina B a una concentración 5 mg/ml, un compuesto capaz de promover la generación de anión superóxido [211] debido a su capacidad para inhibir de la polimerización de la actina y con ello la organización citoesquelética [212,213] Oligonucleótidos utilizados en los ensayos de Q-PCR. Para cada gen, se utilizaron cebadotes directos (Fw) y cebadores inversos (Rv).…”

Section: C212 Valoración Del Anión Superóxidounclassified

Estudio lipídico de la movilización de ácido araquidónico asociada a la respuesta inmune innata

Sesma¹

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Pro‐ and Antioxidant Functions of Quinones and Quinone Reductases in Mammalian Cells

Cadenas¹,

Hochstein²,

Ernster³

1992

Advances in Enzymology - And Related Areas of Molecular Biology

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Inhibitory effect of superoxide-generating quinones on superoxide dismutase

Cited by 24 publications

References 4 publications

The Role of Nitric Oxide and Reactive Oxygen Species in the Killing of Leishmania braziliensis by Monocytes from Patients with Cutaneous Leishmaniasis

The Role of Nitric Oxide and Reactive Oxygen Species in the Killing of Leishmania braziliensis by Monocytes from Patients with Cutaneous Leishmaniasis

Estudio lipídico de la movilización de ácido araquidónico asociada a la respuesta inmune innata

Pro‐ and Antioxidant Functions of Quinones and Quinone Reductases in Mammalian Cells

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