Inhibitory effect of roasted/ unroasted Argania spinosa seeds oil on α- glucosidase, α-amylase and intestinal glucose absorption activities

Daoudi, Nour Elhouda; Ouassou, Hayat; Legssyer, Abdelkhaleq; Mekhfi, Hassane; Ziyyat, Abderrahim; Aziz, Mohammed; Bnouham, Mohamed

doi:10.1016/j.sajb.2020.09.020

Cited by 36 publications

(29 citation statements)

References 40 publications

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“…Other reasons, such as analytical conditions, may also affect this content [12]. The seed oil yield of Opuntia (7 to 15%) is much lower than those reported for other well-known seed oils such as argan kernels (50%), unroasted argan (28.49%), sesame seed (54%), black seed (34%), sunflower (44%), soybean (19%) and olives (20%) [13][14][15][16]. Moreover, the seed oil yield of these two Opuntia species (O. ficus--indica and O. dillenii) is high when compared to others, such as the Xoconostle seeds oil from Mexico (2.45 and 3.52%) [17].…”

Section: Phytochemistry Of Opuntia Oilsmentioning

confidence: 94%

Phytochemistry and biological activities of Opuntia seed oils: Opuntia dillenii (Ker Gawl.) Haw. and Opuntia ficus-indica (L.) Mill. A review

Bouknana

Ouassou

Boutahiri³

et al. 2021

Herba Polonica

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Summary Opuntia species belong to semi-arid and arid regions of Mexico and the United States. O. ficus-indica and O. dillenii are commonly used in alternative medicine to treat various diseases. Up to date, several scientific works have been carried out on the different parts of these plants. However, over the last few years, studies have been focusing on the oil obtained from the fruit seeds of these species. For this reason, this study aims to draw the attention of researchers toward the phytochemical and the pharmacological effects of these two Opuntia oils, which would help set up other scientific projects that promote these products. Phytochemical studies have shown that these oils are rich in biologically active molecules, such as unsaturated fatty acids and phytosterols (mainly linoleic acid and β-sitosterol), as well as vitamin E, which is represented only by the γ-tocopherol. Besides, these oils are rich in polyphenols that protect them from photo-oxidation. Moreover, several studies have shown their antioxidant, anti-diabetic, antibacterial, antifungal, anti-inflammatory, hepatoprotective, and gastroprotective activities, as well as their hypolipidemic properties. The beneficial effects of these oils include also their ability to block the weight loss, and what makes them more interesting is their safety, according to the literature.

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Section: Phytochemistry Of Opuntia Oilsmentioning

confidence: 94%

Phytochemistry and biological activities of Opuntia seed oils: Opuntia dillenii (Ker Gawl.) Haw. and Opuntia ficus-indica (L.) Mill. A review

Bouknana

Ouassou

Boutahiri³

et al. 2021

Herba Polonica

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“…Pancreatic α-amylase inhibitory assay was performed according to the method already described in the literature [ 26 ]. Briefly, 200 µL of plant sample solutions or Acarbose solution (positive control) or phosphate buffer solution (control) was added to 200 µL of the enzyme solution.…”

Section: Methodsmentioning

confidence: 99%

Phytochemical Profile, α-Glucosidase, and α-Amylase Inhibition Potential and Toxicity Evaluation of Extracts from Citrus aurantium (L) Peel, a Valuable By-Product from Northeastern Morocco

et al. 2021

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Due to the high volume of peel produced, Citrus by-product processing could be a significant source of phenolic compounds, in addition to essential oil. Citrus fruit residues, which are usually dumped as waste in the environment, could be used as a source of nutraceuticals. Citrus aurantium (L), also known as sour or bitter orange, is a member of the Rutaceae family and is the result of interspecific hybridization between Citrus reticulata and Citrus maxima. The purpose of this study is to chemically and biologically evaluate the peel of C. aurantium, which is considered a solid waste destined for abandonment. To achieve more complete extraction of the phytochemicals, we used a sequential extraction process with Soxhlet using the increasing polarity of solvents (i.e., cyclohexane, chloroform, ethyl acetate, acetone, and ethanol–water mixture). Essential oil (EO) from the Citrus peel, which was present at 1.12%, was also prepared by hydrodistillation for comparison. Various phytochemical assays were used to determine the qualitative chemical composition, which was subsequently characterized using GC-MS and HPLC-DAD. The inhibitory effects of C. aurantium peel extract on two enzymes, intestinal α-glucosidase and pancreatic α-amylase, were measured in vitro to determine their potential hypoglycemic and antidiabetic actions. Each extract had a significantly different phytochemical composition. According to GC-MS analyses, which allow the identification of 19 compounds, d-limonene is the most abundant compound in both EO and cyclohexane extract, at 35.17% and 36.15 % (w/w). This comparison with hydrodistillation shows the value of the sequential process in extracting this valuable terpene in large quantities while also allowing for the subsequent extraction of other bioactive substances. On the contrary, linoleic acid is abundant (54.35% (w/w)) in ethyl acetate extract (EAE) with a lower amount of d-limonene. HPLC-DAD analysis allows the identification of 11 phytochemicals, with naringenin being the most abundant flavanone, detected in acetone extract (ACE) (23.94% (w/w)), ethanol–water extract mixture (EWE) (28.71% (w/w)), and chloroform extract (CFE) (30.20% (w/w)). Several extracts significantly inhibited α-amylase and/or α-glycosidase in vitro. At a dose of 332 g/mL, ACE, CFE, and EWE inhibited the two enzymes by approximately 98%. There were strong significant correlations between naringenin and α-glucosidase inhibition and between gallic acid and α-amylase inhibition. Molecular docking experiments further verified this. Finally, oral administration of C. aurantium extracts at a dose of 2000 mg/kg did not cause any effect on mice mortality or signs of acute toxicity, indicating that it is non-toxic at these doses. These findings suggest that C. aurantium peels could be a valuable by-product by providing a rich source of non-toxic phytoconstituents, particularly those with potential antidiabetic action that needs to be confirmed in vivo.

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“…The inhibition of pancreatic α-amylase activity by N. sativa seed extracts and fractions was studied according to the procedure described by Daoudi et al [29]. The assay mixtures contained 200 µL of pancreatic α-amylase enzyme solution (13 IU), 200 µL of phosphatebuffer (0.02 M, pH = 6.9) and 200 µL of N. sativa extracts and fractions at two different concentrations (0.5 and 1 mg/mL) or acarbose (positive standard drug control; 0.5 and 1 mg/mL).…”

Section: In Vitro Pancreatic α-Amylase Inhibitory Assaymentioning

confidence: 99%

Linking the Phytochemicals and the α-Glucosidase and α-Amylase Enzyme Inhibitory Effects of Nigella sativa Seed Extracts

Tiji

Bouhrim

Addi

et al. 2021

Foods

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Nigella sativa L. (Ranunculaceae), commonly referred to as black seeds or black cumin, is used in popular medicine (herbal) all over the world for the treatment and prevention of several diseases, including diabetes. This study aims to investigate the inhibitory effect of N. sativa extracts and fractions against the activities of intestinal α-glucosidase and pancreatic α-amylase in vitro, and to explain the inhibitory effect of these fractions against these enzymes by identifying their active compounds responsible for this effect and determine their modes of inhibition. To do so, N. sativa hexane and acetone extracts were prepared and analyzed by GC–MS and HPLC–DAD, respectively. The hexane extract was further fractioned into eight different fractions, while the acetone extract generated eleven fractions. The extracts as well as the resulting fractions were characterized and evaluated for their potential in vitro antidiabetic activity using intestinal α-glucosidase and pancreatic α-amylase inhibitory assays in vitro. Hexane extract and fractions were less active than acetone extract and fractions. In the case of intestinal α-glucosidase activity, the acetone fraction SA3 had a high inhibitory effect on intestinal α-glucosidase activity with 72.26 ± 1.42%, comparable to the effect of acarbose (70.90 ± 1.12%). For the pancreatic α-amylase enzymatic inhibitory assay, the acetone fractions showed an inhibitory capacity close to that for acarbose. In particular, the SA2 fraction had an inhibitory effect of 67.70 ± 0.58% and was rich in apigenin and gallic acid. From these fractions, apigenin, (−)-catechin, and gallic acid were further characterized for their inhibitory actions. IC50 and inhibition mode were determined by analyzing enzyme kinetic parameters and by molecular modeling. Interestingly, (−)-catechin showed a possible synergistic effect with acarbose toward α-glucosidase enzyme inhibition, whereas apigenin showed an additive effect with acarbose toward α-amylase enzymatic inhibition. Furthermore, we studied the toxicity of N. sativa hexane and acetone extracts as well as that of acetone fractions. The result of acute toxicity evaluation demonstrated that N. sativa extracts were nontoxic up to a concentration of 10 g/kg, except for fraction SA3. Taken together, these results indicate that N. sativa extracts and/or derived compounds could constitute promising nutraceuticals for the prevention and treatment of type 2 diabetes mellitus.

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Inhibitory effect of roasted/ unroasted Argania spinosa seeds oil on α- glucosidase, α-amylase and intestinal glucose absorption activities

Cited by 36 publications

References 40 publications

Phytochemistry and biological activities of Opuntia seed oils: Opuntia dillenii (Ker Gawl.) Haw. and Opuntia ficus-indica (L.) Mill. A review

Phytochemistry and biological activities of Opuntia seed oils: Opuntia dillenii (Ker Gawl.) Haw. and Opuntia ficus-indica (L.) Mill. A review

Phytochemical Profile, α-Glucosidase, and α-Amylase Inhibition Potential and Toxicity Evaluation of Extracts from Citrus aurantium (L) Peel, a Valuable By-Product from Northeastern Morocco

Linking the Phytochemicals and the α-Glucosidase and α-Amylase Enzyme Inhibitory Effects of Nigella sativa Seed Extracts

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