Inhibitory Effect of Rikkunshi-To, a Traditional Chinese Herbal Prescription, on Tumor Promotion in Two-Stage Carcinogenesis in Mouse Skin.

Abstract

We report the inhibitory effect of topical application of extracts of a traditional Chinese herbal prescription on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice. Methanol and water extracts obtained from 22 traditional Chinese herbal prescriptions were assayed and their inhibition ratios calculated. In general, the methanol extracts produced more effective inhibition than the water extracts. Of the various traditional Chinese herbal prescriptions, the methanol extract of Rikkunshi-to was more effective than other prescriptions as far as inhibition of TPA-induced inflammation was concerned. Hoelen, Glycyrrhizae Radix, Atractylodis Rhizoma, components of Rikkunshi-to markedly inhibited the inflammatory activity involved by TPA in mice. Furthermore, topical application of the methanol extract of Rikkunshi-to markedly inhibited TPA-induced tumor promotion in two-stage carcinogenesis in mouse skin.

Inhibitory Effect of Oral Administration of Monascus Pigment on Tumor Promotion in Two-Stage Carcinogenesis in Mouse Skin

“…Therefore, foods and food additives may prove to be important for the chemoprevention of cancer. Our pre vious studies have demonstrated that oral administration of cepharanthine can inhibit tumor promotion during twostage carcinogenesis in mouse skin [6], In addition, Rikkunshi-to, a traditional Chinese herbal prescription, inhib its tumor promotion in two-stage carcinogenesis in mouse skin [7], Mice were treated with 0.1 and 0.02% Monascus pigment as their sole source of drinking water starting 1 day after initiation by DMBA and continuing throughout the tumor promotion regimen until the end of the study. Oral administration of Monascus pigment inhibits tumor promotion by TPA in DMBA-initiated mice.…”

Inhibitory Effects of Lanostane-Type Triterpene Acids, the Components of <i>Poria cocos</i>, on Tumor Promotion by 12-O-Tetradecanoylphorbol-13-Acetate in Two-Stage Carcinogenesis in Mouse Skin

“…These drugs are used in the treatment of immunosuppression caused by anticancer drugs and poor health after surgical treatment. In addition, Rikkunshi-to and Zyuzen-taiho-to inhibited the tumor promotion in two-stage carcinogenesis in mouse skin [21,22]. P. cocos was found to be an important component in these pre scriptions.…”

Inhibitory Effect of Eucommia Bark on Tumour Formation in a Mouse Model of Two-Stage Skin Carcinogenesis

“…In our study, methanol extract was suitable for extraction of the active components than water extract 9) . The reason that used methanol is because we expected active component to a low molecular compound.…”