Inhibitory effect of riccardin D on growth of human non-small cell lung cancer: In vitro and in vivo studies

Xue, Xindong; Sun, De-Fu; Sun, Cuicui; Liu, Huiping; Yue, Bin; Zhao, Cuirong; Lou, Hongxiang; Qu, Xian‐Jun

doi:10.1016/j.lungcan.2011.12.013

Cited by 28 publications

(12 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cell surface phosphatidylserine in apoptotic cells was quantitatively estimated by using Annexin V/FITC and 7-AAD apoptosis detection kit according to manufacturer's instructions (Roche, USA). Cell apoptosis was analyzed on a FACScan flow cytometry (Becton Dickinson, USA) (Xue et al, 2012). Triplicate experiments with triplicate samples were performed.…”

Section: Annexin V/fitc and 7-aad Staining Analysismentioning

confidence: 99%

Curcumin Induces Apoptosis in SGC-7901 Gastric Adenocarcinoma Cells via Regulation of Mitochondrial Signaling Pathways

Xue¹,

Yu²,

Sun³

et al. 2014

Asian Pacific Journal of Cancer Prevention

Self Cite

View full text Add to dashboard Cite

Curcumin, a polyphenol compound derived from the rhizome of the plant Curcuma longa L. has been verified as an anticancer compound against several types of cancer. However, understanding of the molecular mechanisms by which it induces apoptosis is limited. In this study, the anticancer efficacy of curcumin was investigated in human gastric adenocarcinoma SGC-7901 cells. The results demonstrated that curcumin induced morphological changes and decreased cell viability. Apoptosis triggered by curcumin was visualized using Annexin V-FITC/7-AAD staining. Curcumin-induced apoptosis of SGC-7901 cells was associated with the dissipation of mitochondrial membrane potential (MMP) and the release of cytochrome c into the cytosol. Furthermore, the down-regulation of Bcl-2 and up-regulation of Bax that led to the cleavage of caspase-3 and increased cleaved PARP was observed in SGC-7901 cells treated with curcumin. Therefore, curcumin-induced apoptosis of SGC-7901 cells might be mediated through the mitochondria pathway, which gives the rationale for in vivo studies on the utilization of curcumin as a potential cancer therapeutic compound.

show abstract

Section: Annexin V/fitc and 7-aad Staining Analysismentioning

confidence: 99%

Curcumin Induces Apoptosis in SGC-7901 Gastric Adenocarcinoma Cells via Regulation of Mitochondrial Signaling Pathways

Xue¹,

Yu²,

Sun³

et al. 2014

Asian Pacific Journal of Cancer Prevention

Self Cite

View full text Add to dashboard Cite

show abstract

“…tosana was found to induce apoptosis in MCF-7 cells [ 12 ]. A macrocyclic bisbibenzyl, riccardin D, which was isolated from Dumortiera hirsuta inhibited the proliferation of human non-small-cell lung cancer (NSCLC) both in vitro and in vivo and does not cause toxicity in normal mammalian cells [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort,Lepidozia borneensisStephani from Mount Kinabalu, Sabah, Malaysia

Bakar

Suleiman

et al. 2015

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

The study aimed to investigate the phytochemical contents, antioxidant and antiproliferative activity of 80% methanol extract of Lepidozia borneensis. The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods. Antioxidant properties were evaluated by using FRAP, ABTS, and DPPH assays while the effects of L. borneensis on the proliferation of MCF-7 cell line were evaluated by using MTT assay. The results showed that the total phenolic and flavonoid contents were 12.42 ± 0.47 mg GAE/g and 9.36 ± 1.29 mg CE/g, respectively. The GC-MS analysis revealed the presence of at least 35 compounds. The extract was found to induce cytotoxicity against MCF-7 cell line with IC50 value of 47.33 ± 7.37 µg/mL. Cell cycle analysis showed that the extract induced significant arrest at G0/G1 at 24 hours of treatment. After 72 hours of treatment, the proportion of cells in G0/G1 and G2-M phases had decreased significantly as compared to their control. Apoptosis occurred during the first 24 hours and significantly increased to 30.8% after 72 hours of treatment. No activation of caspase 3 was observed. These findings suggest that L. borneensis extract has the potential as natural antioxidant and anticancer agents.

show abstract

“…Since RD has been found to induce apoptosis in leukemia cells and lung cancer cells [16,18], we initiated our study to determine whether RD also exerted cytotoxic effect on PCa cells by continuously monitoring cell proliferation. As shown in Figure 1A, exposure of PC-3 cells to RD caused markedly suppression of cell proliferation in a dose- and time-dependent manner.…”

Section: Resultsmentioning

confidence: 99%

Riccardin D Exerts Its Antitumor Activity by Inducing DNA Damage in PC-3 Prostate Cancer Cells In Vitro and In Vivo

Kong

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

We recently reported that Riccardin D (RD) was able to induce apoptosis by targeting Topo II. Here, we found that RD induced cell cycle arrest in G2/M phase in PC-3 cells, and caused remarkable DNA damage as evidenced by induction of γH2AX foci, micronuclei, and DNA fragmentation in Comet assay. Time kinetic and dose-dependent studies showed that ATM/Chk2 and ATR/Chk1 signaling pathways were sequentially activated in response to RD. Blockage of ATM/ATR signaling led to the attenuation of RD-induced γH2AX, and to the partial recovery of cell proliferation. Furthermore, RD exposure resulted in the inactivation of BRCA1, suppression of HR and NHEJ repair activity, and downregulation of the expressions and DNA-end binding activities of Ku70/86. Consistent with the observations, microarray data displayed that RD triggered the changes in genes responsible for cell proliferation, cell cycle, DNA damage and repair, and apoptosis. Administration of RD to xenograft mice reduced tumor growth, and coordinately caused alterations in the expression of genes involved in DNA damage and repair, along with cell apoptosis. Thus, this finding identified a novel mechanism by which RD affects DNA repair and acts as a DNA damage agent in prostate cancer.

show abstract

Inhibitory effect of riccardin D on growth of human non-small cell lung cancer: In vitro and in vivo studies

Cited by 28 publications

References 41 publications

Curcumin Induces Apoptosis in SGC-7901 Gastric Adenocarcinoma Cells via Regulation of Mitochondrial Signaling Pathways

Curcumin Induces Apoptosis in SGC-7901 Gastric Adenocarcinoma Cells via Regulation of Mitochondrial Signaling Pathways

Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort,Lepidozia borneensisStephani from Mount Kinabalu, Sabah, Malaysia

Riccardin D Exerts Its Antitumor Activity by Inducing DNA Damage in PC-3 Prostate Cancer Cells In Vitro and In Vivo

Contact Info

Product

Resources

About