Inhibitory effect of quercetin on rat trachea contractility &lt;I&gt;in vitro&lt;/I&gt;

Capasso, Raffaele; Aviello, Gabriella; Romano, Barbara; Atorino, Giuseppina; Pagano, Ester; Borrelli, Francesca

doi:10.1211/jpp/61.01.0016

Cited by 17 publications

(8 citation statements)

References 31 publications

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“…This is in good agreement with other studies where MAPK inhibitors restored altered intestinal transit in burned rats [40] or the beneficial effect of antioxidants in the LPS‐induced motility disorders associated with a reduction in MAPK activation [11] . In fact, antioxidants are being extensively used to restore the altered muscle response although sometimes they have effects per se on contractility [41] . MAPKs seem to be an important focus for the therapy of various diseases involving intestinal motility disorders such as inflammatory bowel disease [18] or postoperative ileus [42] …”

Section: Discussionsupporting

confidence: 90%

Role of TLR4 and MAPK in the local effect of LPS on intestinal contractility†

Barona

Fagundes

Gonzálo

et al. 2011

Journal of Pharmacy and Pharmacology

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Objectives Lipopolysaccharide (LPS) has been shown to alter intestinal contractility. Toll-like receptor 4 (TLR4), K + channels and mitogen-activated protein kinases (MAPKs) have been proposed to be involved in the mechanism of action of LPS. The aim of this study was to determine the role of TLR4, K + channels and MAPKs (p38, JNK and MEK1/2) in the local effect of LPS on the acetylcholine (ACh)-induced contractions in rabbit small intestine in vitro. Methods Segments of rabbit duodenum were suspended in the direction of longitudinal or circular smooth muscle fibres in a thermostatically controlled organ bath. Key findings LPS (0.3 mg/ml) reduced the contractions induced by ACh (100 mm) in the longitudinal and circular smooth muscle of the duodenum after 90 min of incubation. Polymyxin (TLR4 inhibitor), SB203580 (p38 MAPK inhibitor), SP600125 (JNK1/2 inhibitor) and U0126 (MEK1/2 inhibitor) antagonized the effects of the LPS on ACh-induced contractions in duodenal smooth muscle. Incubation with the blockers of K + channels, TEA, apamin, charybdotoxin, iberiotoxin, glibenclamide or quinine, did not reverse the effect of LPS on ACh-induced contractions. Conclusions These results suggest that the effect of LPS on ACh-induced contractions in the rabbit duodenum might be mediated by TLR4 and p38, JNK1/2 and MEK1/2 MAPKs.

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Section: Discussionsupporting

confidence: 90%

Role of TLR4 and MAPK in the local effect of LPS on intestinal contractility†

Barona

Fagundes

Gonzálo

et al. 2011

Journal of Pharmacy and Pharmacology

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“…In 2009, the effect of quercetin on isolated tracheal tissue from male Wistar rats has been evaluated by Capasso et al They showed quercetin (in concentrations of 10 −6 -3 × 10 −4 M) inhibits rat tracheal contractility through a presynaptic and a postsynaptic site of action. They concluded that quercetin could be considered as a possible application in airways diseases such as asthma [24]. Two studies have been investigated the effect of quercetin on MUC5AC in allergic asthma.…”

Section: Effect Of Quercetin On Allergic Asthma In Vitro Studiesmentioning

confidence: 99%

Quercetin with the potential effect on allergic diseases

Jafarinia

Hosseini

Kasiri

et al. 2020

Allergy Asthma Clin Immunol

112

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Quercetin is a naturally occurring polyphenol flavonoid which is rich in antioxidants. It has anti-allergic functions that are known for inhibiting histamine production and pro-inflammatory mediators. Quercetin can regulate the Th1/ Th2 stability, and decrease the antigen-specific IgE antibody releasing by B cells. Quercetin has a main role in antiinflammatory and immunomodulatory function which makes it proper for the management of different diseases. Allergic diseases are a big concern and have high health care costs. In addition, the use of current therapies such as ß2-agonists and corticosteroids has been limited for long term use due to their numerous side effects. Since the effect of quercetin on allergic diseases has been widely studied, in the current article, we review the effect of quercetin on allergic diseases, such as allergic asthma, allergic rhinitis (AR), and atopic dermatitis (AD).

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“…Ko et al [48] studied the broncho-relaxant effect of quercetin, rutin, kaempferol, apigenin, and hesperidin on KCl (30 mM), and carbachol (0.2 µM) induced spastic contraction on isolated guinea pig trachea preparations. Capasso et al [49] studied the bronchodilator effect of quercetin on rat trachea. Djelili et al [50] reported that quercetin and rutin had a bronchodilator effect on isolated human bronchus.…”

Section: Discussionmentioning

confidence: 99%

Cucumis sativus L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway

et al. 2021

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Cucumis sativus L. is globally cultivated as an edible vegetable. Besides its nutritional benefits, it is used in traditional medicines against various ailments. The current study was designed to elucidate the multi-target mechanisms of a C. sativus seeds extract against asthma and diarrhea using network pharmacology along with a molecular docking approach. Furthermore, in-vitro and in-vivo experiments were conducted to verify the mechanistic insight of in silico studies. LC-ESI-MS/MS was performed to identify the bioactive compounds in the extract; later, some compounds were quantified by HPLC. C. sativus seed. EtOH has kaempferol in higher concentration 783.02 µg/g, followed by quercetin (693.83 µg/g) and luteolin (617.17 µg/g). In silico studies showed that bioactive compounds interfered with asthma and diarrhea-associated target genes, which are members of calcium-mediated signaling to exert a calcium channel blocker activity. The seeds extract exerted a concentration-dependent spasmolytic response on isolated jejunum, trachea, and urinary bladder preparations and caused relaxation of spastic contraction of K+ (80 mM) with suppressed calcium concentration-response curves at dose 0.3 and 1 mg/mL. It also showed antiperistalsis, antidiarrheal and antisecretory activity in animal models. Thus, C. sativus seeds have therapeutic effects by regulating the contractile response through a calcium-mediated signaling pathway.

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Inhibitory effect of quercetin on rat trachea contractility <I>in vitro</I>

Abstract: Quercetin inhibits rat tracheal contractility through a presynaptic (involving nitric oxide) and a postsynaptic site of action.

Cited by 17 publications

References 31 publications

Role of TLR4 and MAPK in the local effect of LPS on intestinal contractility†

Role of TLR4 and MAPK in the local effect of LPS on intestinal contractility†

Quercetin with the potential effect on allergic diseases

Cucumis sativus L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway

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