Inhibitory effect of PVDF tubes on biofilm formation in dental unit waterlines

Yabune, Toshiaki; Imazato, Satoshi; Ebisu, Shigeyuki

doi:10.1016/j.dental.2005.01.016

Cited by 25 publications

(33 citation statements)

References 26 publications

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“…A surface coated with PVDF has a low free energy (38), and PVDF inhibits the attachment of organic materials in a nonspecific manner similarly to other fluoridated surfaces (3,31). In the present study, the use of the PVDF-coated tube inhibited the attachment of each bacterium identified from water and biofilm samples in DUWLs, resulting in the interruption of the biofilm-forming processes even when the incubation conditions were optimal to produce a mature biofilm on a conventional tube.…”

Section: Discussionmentioning

confidence: 78%

“…Using clinical tests, we have previously reported that contamination of DUWLs can be effectively reduced by using a PVDF-coated tube instead of a conventional polyurethane tube (38). A surface coated with PVDF has a low free energy (38), and PVDF inhibits the attachment of organic materials in a nonspecific manner similarly to other fluoridated surfaces (3,31).…”

Section: Discussionmentioning

confidence: 99%

“…Increased risks of pyrogenic and septicemic complications have been reported when the level of contamination exceeds 10,000 bacteria/ml in dialysis fluids (11). Considering the total number of bacteria in DUWLs equipped with conventional polyurethane tubes (1,8,15,22,24,34,36,38) and the population determined in this study, the amount of S. paucimobilis discharged can be calculated to be greater than 10,000 CFU/ml. Although no information is available on the definitive concentration of S. paucimobilis that causes symptomatic infection, more attention should be paid to reduce its outflow from DUWLs to eliminate the risk of opportunistic infections.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, an in vitro model can provide a powerful tool for developing fundamental measures to prevent DUWL contamination. Such a model will enable the screening of the effectiveness of various preventive methods in a short period, while the present clinical usage tests are time-consuming and have been shown to give nonreproducible results (8,10,14,15,20,38).…”

mentioning

confidence: 99%

“…In addition, using the established in vitro model, the inhibitory effects of a polyvinylidene fluoride (PVDF)-coated tube, which was previously demonstrated to be effective in reducing bacterial outflow from DUWLs in a clinical study (38), were evaluated.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Assessment of Inhibitory Effects of Fluoride-Coated Tubes on Biofilm Formation by Using the In Vitro Dental Unit Waterline Biofilm Model

Yabune

Imazato

Ebisu

2008

Appl Environ Microbiol

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This study aimed to establish an in vitro model to simulate biofilms formed in dental unit waterlines (DUWLs) and to investigate the ability of polyvinylidene fluoride (PVDF)-coated tubes to inhibit biofilm formation using this model. The water and biofilm samples were obtained from DUWLs which had been clinically used for 2.5 years, and the predominant bacteria were identified. A conventional polyurethane tube was incubated for 24 to 96 h in the mixed flora of isolated bacteria, and the optimal incubation conditions to simulate a clinically formed biofilm were determined by observation with a scanning electron microscope. Biofilm formation on a PVDF-coated tube was observed using this in vitro model, and the adherence of different bacterial species to conventional and PVDF-coated tubes was assessed. Sphingomonas paucimobilis, Acinetobacter haemolytics, and Methylobacterium mesophilicum were predominantly isolated from contaminated DUWLs. Incubation of the polyurethane tube with the mixed flora containing these three species for 96 h resulted in the formation of a mature biofilm similar to the one clinically observed. The PVDF-coated tube was significantly less adhesive to all three bacterial species than the polyurethane tube (P < 0.05 by the MannWhitney U test), and the attachment of small amounts of rods was observed even after incubation with the mixed flora for 96 h. In conclusion, an in vitro biofilm model was obtained by using a mixed flora of bacteria isolated from DUWLs, and the PVDF-coated tube was found to be effective in preventing biofilm formation using this model.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Assessment of Inhibitory Effects of Fluoride-Coated Tubes on Biofilm Formation by Using the In Vitro Dental Unit Waterline Biofilm Model

Yabune

Imazato

Ebisu

2008

Appl Environ Microbiol

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Biofilm‐Related Infections in the Oral Cavity

Shunmugaperumal

2010

Biofilm Eradication and Prevention

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Electrospinning of antibacterial poly(vinylidene fluoride) nanofibers containing silver nanoparticles

Yuan

Geng

Xing

et al. 2009

J of Applied Polymer Sci

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Poly(vinylidene fluoride) (PVDF) nanofibrous mats containing silver nanoparticles were prepared by electrospinning. The diameter of the nanofibers ranged between 100 and 300 nm, as revealed by scanning electron microscopy. The silver nanoparticles were dispersed, but some aggregation was observed with transmission electron microscopy. The content of silver nanoparticles incorporated into the PVDF nanofibrous mats was determined by inductively coupled plasma and X-ray photoelectron spectroscopy. The antibacterial activities of the samples were evaluated with the colony-counting method against Staphylococcus aureus (Grampositive) and Klebsiella pneumoniae (Gram-negative) bacteria. The results indicate that the PVDF nanofibrous mats containing silver nanoparticles showed good antibacterial activity compared to the PVDF nanofiber control.

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Inhibitory effect of PVDF tubes on biofilm formation in dental unit waterlines

Cited by 25 publications

References 26 publications

Assessment of Inhibitory Effects of Fluoride-Coated Tubes on Biofilm Formation by Using the In Vitro Dental Unit Waterline Biofilm Model

Assessment of Inhibitory Effects of Fluoride-Coated Tubes on Biofilm Formation by Using the In Vitro Dental Unit Waterline Biofilm Model

Biofilm‐Related Infections in the Oral Cavity

Electrospinning of antibacterial poly(vinylidene fluoride) nanofibers containing silver nanoparticles

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