Inhibitory Effect of Protease-Inhibitor for Production of Interleukin 8 (IL-8) and Polymorphonuclear Leukocyte Elastase (Pmne)

Effects of Urinary Trypsin Inhibitor on Lipopolysaccharide-Induced Acute Lung Injury in Rabbits

“…However, LPS-induced neutrophils infiltration of lung was attenuated in the Pre-UTI high group. Previous studies reported that UTI inhibits LPS-induced production of IL-8 in vascular endothelial cells [21], neutrophil elastase-induced IL-8 gene expression [22,23], expression of intercellular adhesion molecule-1, endothelial cell adhesion molecule-1 on endothelial cells, and transendothelial migration of neutrophils stimulated by IL-8 [24]. Also, UTI not only inactivated elastase secreted by neutrophils, but also suppressed the production and secretion of elastase from neutrophils which play an important role of diapedesis and extravasation of granulocytes [21,25,26].…”

“…Previous studies reported that UTI inhibits LPS-induced production of IL-8 in vascular endothelial cells [21], neutrophil elastase-induced IL-8 gene expression [22,23], expression of intercellular adhesion molecule-1, endothelial cell adhesion molecule-1 on endothelial cells, and transendothelial migration of neutrophils stimulated by IL-8 [24]. Also, UTI not only inactivated elastase secreted by neutrophils, but also suppressed the production and secretion of elastase from neutrophils which play an important role of diapedesis and extravasation of granulocytes [21,25,26]. Even though we did not prove directly that UTI decreases the production of IL-8 in specific cells such as macrophage or epithelial cell and decreases migration of neutrophils (chemotatic effects) into lung, it is likely that UTI attenuated the histopathologic severity of lung parenchyma through the inhibition of neutrophil migration into the lung parenchyma as shown in our study.…”

Induction of acute-phase reactive substances during open-heart surgery and efficacy of ulinastatin

“…27 It was recently reported that, in vitrous studies, UST inhibited PMNE release by suppressing the production of TNF, IL-6, and IL-8. 6 Furunaga and his colleagues 28 assumed that anticytokine therapy and aggressive administration of UST were useful against postoperative complications in patients with cardiac surgery especially, after longterm CPB, because they were considered to be facing multiple organ failure. The dose of UST and the timing of administration remain controversial.…”

Urinary Trypsin Inhibitor, an Alternative Therapeutic Option for Inflammatory Disorders

“…Beyond its inhibition of inflammatory proteases mentioned above, UTI exhibits antiinflammatory activity and suppresses the infiltration of neutrophils and release of elastase and chemical mediators from them [11,20,21]. Likewise, UTI reportedly inhibits the production of tumor necrosis factor (TNF)- [22,23] and interleukin (IL)-1 [23] in LPSstimulated human monocytes and LPS-or neutrophil elastase-stimulated IL-8 gene expression in HL60 cells [24] or bronchial epithelial cells [25] in vitro.…”