Inhibitory Effect of Powdered &lt;i&gt;Morus australis &lt;/i&gt;Leaves on Postprandial Elevation of Blood Glucose

Kume, Daisuke; Fukami, Arisa; Kuraya, Eisuke; Shimajiri, Yoshinori; Ito, Masaaki

doi:10.3136/nskkk.66.52

Cited by 3 publications

(9 citation statements)

References 7 publications

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“…It contains more 1‐DNJ than Morus sp. In Japanese main island (Kume et al., 2019). Consumption of the M. australis leaf tea suppressed sucrose‐induced increase in blood glucose and insulin levels in human (Kume et al., 2019, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…In Japanese main island (Kume et al, 2019). Consumption of the M. australis leaf tea suppressed sucroseinduced increase in blood glucose and insulin levels in human (Kume et al, 2019(Kume et al, , 2021. We previously examined the inhibitory effects of powdered M. australis leaf extract and its component iminosugars 1-DNJ, fagomine, and 2-O-α-D-galactopyranosyl deoxynojirimycin (GAL-DNJ) (Qiao et al, 2020(Qiao et al, , 2021, showing that 1-DNJ is a major inhibitor of α-glucosidase in the M. australis leaf extract.…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of α‐amylase and α‐glucosidase by Morus australis fruit extract and its components iminosugar, anthocyanin, and glucose

Qiao

Ikeda

Ito

et al. 2022

Journal of Food Science

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The inhibition of α‐amylase and α‐glucosidase are important for the maintenance of blood glucose level. Mammalian α‐glucosidase includes maltase–glucoamylase and sucrase–isomaltase complexes. In this study, we examined the inhibitory effects of Morus australis fruit extract and its components, that is, three iminosugars (1‐deoxynojirimycin [1‐DNJ], fagomine, and 2‐O‐α‐D‐galactopyranosyl deoxynojirimycin), two anthocyanins (cyanidin‐3‐glucoside and cyanidin‐3‐rutinoside), and glucose, against α‐amylase and α‐glucosidase. We also quantified the concentration of each component in M. australis fruit extract. The IC50 values of the fruit extracts of four M. australis subspecies were >10 mg/ml for α‐amylase, 1.1–1.7 mg/ml for maltase, 6.9–8.6 mg/ml for glucoamylase, 0.13–1.0 mg/ml for sucrase, and 0.46–1.4 mg/ml for isomaltase. When the IC50 value of each component and the concentration of each component in the fruit extract were taken into consideration, our results indicated that glucose are involved in the inhibition of α‐amylase, and 1‐DNJ and glucose are involved in the inhibition of α‐glucosidase. This is in contrast to that in M. australis leaf, neither anthocyanin nor glucose are contained, and 1‐DNJ is a main inhibitor. Practical Application It is widely accepted that inhibition of α‐amylase and α‐glucosidase is one of the strategies to treat type‐2 diabetes. Today, acarbose, miglitol, and voglibose are clinically used for this purpose. Our results that 1‐DNJ and anthocyanin are present in Morus australis fruit and are involved in the inhibition of α‐amylase and α‐glucosidase suggest that M. australis fruit is a healthy sweetener.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inhibition of α‐amylase and α‐glucosidase by Morus australis fruit extract and its components iminosugar, anthocyanin, and glucose

Qiao

Ikeda

Ito

et al. 2022

Journal of Food Science

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“…The ingestion of the tea suppressed sucrose-induced elevation of blood glucose and insulin levels, indicating that the tea inhibited postprandial elevation of blood glucose. 11) In the inhibition of the hydrolysis of sucrose by sucrase from rat intestine, boiled water extract of powdered M. australis leaves exhibited the IC50 value of 12 ± 1.3 µg/mL. 11) These results suggest that a high intake of M. australis tea might be effective for the prevention of type-2 diabetes.…”

Section: Introductionmentioning

confidence: 97%

“…11) In the inhibition of the hydrolysis of sucrose by sucrase from rat intestine, boiled water extract of powdered M. australis leaves exhibited the IC50 value of 12 ± 1.3 µg/mL. 11) These results suggest that a high intake of M. australis tea might be effective for the prevention of type-2 diabetes. Recently, various physiological activities of 1-DNJ have been reported, such as suppression of the elevation of postprandial blood glucose, 13) postprandial glycemic control in subjects with impaired glucose metabolism, 14) stimulation of adiponectin and GLUT4 expressions adipocytes, 15) hypoglycemic effect, 16) prevention of diet-induced obesity through increases in adiponectin, 17) and suppression of lipid accumulation through activation of the βoxidation system.…”

Section: Introductionmentioning

confidence: 97%

“…It was reported that the 1-DNJ concentrations in M. australis leaves were 4 to 9-fold higher than those in Morus alba leaves distributed in Japanese mainland. 10,11) Powdered and roasted M. australis leaves were developed as a tea with a function to inhibit α-glucosidase activity. The ingestion of the tea suppressed sucrose-induced elevation of blood glucose and insulin levels, indicating that the tea inhibited postprandial elevation of blood glucose.…”

Section: Introductionmentioning

confidence: 99%

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Kinetic analysis of inhibition of α-glucosidase by leaf powder from Morus australis and its component iminosugars

Qiao

Nakayama

Ikeuchi

et al. 2020

Bioscience, Biotechnology, and Biochemistry

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Mulberry leaves contain iminosugars, such as 1-deoxynojirimycin (1-DNJ), fagomine, and 2-O-α-D-galactopyranosyl deoxynojirimycin (GAL-DNJ) that inhibit α-glucosidase. In this study, we quantified iminosugars in Morus australis leaves and made the kinetic analysis in the hydrolysis of maltose by α-glucosidase. By LC-MS/MS, the concentrations of 1-DNJ, fagomine, and GAL-DNJ in the powdered leaves were 4.0, 0.46, and 2.5 mg/g, respectively, and those in the roasted ones were 1.0, 0.24, and 0.73 mg/g, respectively, suggesting that the roasting process degraded iminosugars. Steady-state kinetic analysis revealed that the powdered and roasted leaves exhibited competitive inhibition. At pH 6.0 at 37ºC, the IC50 values of the extracts from the boiled powdered or roasted leaves were 0.36 and 1.1 mg/mL, respectively. At the same condition, the IC50 values of 1-DNJ, fagomine, and GAL-DNJ were 0.70 μg/mL, 0.18 mg/mL, and 2.9 mg/mL, respectively. These results suggested that in M. australis, 1-DNJ is a major inhibitor of α-glucosidase. Abbreviations 1-DNJ: 1-deoxynojirimycin; GAL-DNJ: 2-O-α-D-galactopyranosyl-DNJ

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Inhibition of α-glucosidase activity by Morus australis fruit food

et al. 2023

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Mulberry fruit is thought as a healthy sweetener because it contains iminosugars, such as 1deoxynojirimycin (1-DNJ), fagomine and 2-O-α-D-galactopyranosyl deoxynojirimycin (GAL-DNJ), that inhibits α-glucosidase. In this study, we quantified iminosugars and glucose concentrations in Morus australis fruit foods and analyzed their inhibitory effects against the activities of αglucosidase-related four enzymes, maltase, glucoamylase, sucrase, and isomaltase. By LC-MS/MS, the concentrations of 1-DNJ, fagomine, and GAL-DNJ of the M. australis fruit wine, juice, vinegar drink, vinegar, and jam were 1.76-32.5, 0.058-0.290, and 30.2-209 μg/mL, respectively. By highperformance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD),the glucose concentrations were 0 mg/mL for wine and 13.9-39.7 mg/mL for juice, vinegar drink, vinegar, and jam. The IC50 values of these five foods were 1.9-4.4 % v/v for maltase, 0.37-5.0 % for glucoamylase, and 0.28-1.2 % for sucrase, and 2.0% for isomaltase. Considering the 1-DNJ and glucose concentrations in these five M. australis fruit foods and the IC50 values of 1-DNJ and glucose for α-glucosidase, it was suggested that 1-DNJ is a main inhibitor for α-glucosidase in these foods, and glucose is involved in the inhibition except for wine.

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Inhibitory Effect of Powdered <i>Morus australis </i>Leaves on Postprandial Elevation of Blood Glucose

Cited by 3 publications

References 7 publications

Inhibition of α‐amylase and α‐glucosidase by Morus australis fruit extract and its components iminosugar, anthocyanin, and glucose

Inhibition of α‐amylase and α‐glucosidase by Morus australis fruit extract and its components iminosugar, anthocyanin, and glucose

Kinetic analysis of inhibition of α-glucosidase by leaf powder from Morus australis and its component iminosugars

Inhibition of α-glucosidase activity by Morus australis fruit food

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