Inhibitory effect of Physalis alkekengi L. var. franchetii extract and its chloroform fraction on LPS or LPS/IFN-γ-stimulated inflammatory response in peritoneal macrophages

Kang, Hee; Kwon, Seung-Ro; Choi, Ho‐Young

doi:10.1016/j.jep.2011.02.028

Cited by 27 publications

(16 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The herbal preparations of CSFP are traditionally used to treat cough, sore throat, abscesses, and urinary problems. Currently, several pharmacological effects are reported such as antimicrobial [5], anti-inflammatory [6], antitumor [7], and so on, which might attribute to those identified compounds such as physalins, flavones, alkaloids, and so on [8]. The decoction of CSFP is believed to be useful as an antihyperlipidemic preparation.…”

Section: Introductionmentioning

confidence: 99%

Bioactivity-guided fractionation of the triglyceride-lowering component and in vivo and in vitro evaluation of hypolipidemic effects of Calyx seu Fructus Physalis

et al. 2012

View full text Add to dashboard Cite

BackgroundIn folklore, some people take the decoction of Calyx seu Fructus Physalis (CSFP) for lowering blood lipids. The present study is designed to evaluate the lipid-lowering activities of CSFP, and search for its pharmacodynamical material.MethodsCSFP was extracted by water and 75% ethanol, respectively. The extracts of CSFP for reducing serum lipid levels were evaluated on mouse model of hyperlipidemia. The optimized extract was subjected to the bioactivity-guided fractionation in which the liquid-liquid extraction, collumn chromatography, the in vivo and in vitro models of hyperlipidemia were utilized. The structure of active component was determined by 13 C-NMR and 1H-NMR.ResultsThe 75% ethanol extract of CSFP decreased the serum total cholesterol (TC) and triglyceride (TG) levels in mouse model of hyperlipidemia. Followed a separation process for the 75% ethanol extract of CSFP, the fraction B was proved to be an active fraction for lowering lipid in vivo and in vitro experiments, which could significantly decrease the serum TC and TG levels in mouse model of hyperlipidemia, and remarkably decrease the increase of TG in primary mouse hepatocytes induced by high glucose and the increase of TG in HepG2 cells induced by oleic acid. The fraction B2, isolated from B on bioactivity-guided fractionation, could significantly decrease TG level in HepG2 cells. One compound with the highest content in B2 was isolated and determined as luteolin-7-O-beta-D-glucopyranoside by NMR spectra. It could significantly reduce the TG level in HepG2 cells, and inhibited the accumulation of lipids by oil red O stain.ConclusionOur results demonstrated that the 75% ethanol extract of CSFP could improve in vitro and in vivo lipid accumulation. Luteolin-7-O-beta-D-glucopyranoside might be a leading pharmacodynamical material of CSFP for lowering lipids.

show abstract

Section: Introductionmentioning

confidence: 99%

Bioactivity-guided fractionation of the triglyceride-lowering component and in vivo and in vitro evaluation of hypolipidemic effects of Calyx seu Fructus Physalis

et al. 2012

View full text Add to dashboard Cite

show abstract

“…PA has been consumed for its many beneficial effects in traditional medicine and reportedly has many beneficial properties. Previous literature has reported the antifertility properties of PA, 9 anti-inflammatory properties, 10,11 and even anticancer properties. 12 Other than that, reports have also revealed the antihyperglycemic activity of PA. 3,13 Some studies have also reported its antidiabetic activities.…”

Section: Discussionmentioning

confidence: 99%

Physalis alkekengi Exhibits Antiobesity Effects in Mice with Potential of Inducing White Adipose Tissue Browning

Lee

Kim

Irfan

et al. 2020

Journal of Medicinal Food

View full text Add to dashboard Cite

The aim of this study was to investigate the efficacy of an ethanol extract of Physalis alkekengi (PA) and its mechanistic pathway of action at the molecular level for its antiobesity properties. Four-week old male Institute of Cancer Research (ICR) mice were acclimatized for a week before starting the high-fat diet (HFD) for 2 weeks to induce obesity, followed by 8 more weeks of oral administration of 10 mg/kg orlistat and 300 mg/kg of PA extract, along with HFD. Body weights of the mice and feed and water intake were recorded weekly. After a total of 12 weeks, mice were euthanized, and blood, liver, and adipose tissues were harvested for further analysis. Administration of PA extract inhibited the progression of obesity by reducing weight gain, weight of adipose tissue, and normalizing serum triglyceride, glucose, total cholesterol, highdensity lipoprotein, low-density lipoprotein, alanine aminotransferase, and aspartate aminotransferase. PA extract prevented the progression of nonalcoholic steatohepatitis induced by HFD and prevented the enlargement of liver. Phosphorylation of adenosine monophosphate-activated protein kinase a increased while phosphorylation of acetyl-CoA carboxylase was reduced. The browning gene uncoupling protein 1 expression was also increased by PA extract treatment. Our findings revealed that the antiobesity properties of PA extract may be mediated by browning of white adipose tissue.

show abstract

“…It is widely used for the treatment and prevention of different diseases, including sore throat, cough, eczema, tonsillitis, pharyngitis, hepatitis, leishmaniasis and tumours [2]. Recently, several studies show that it plays a critical role in anti-tumour, anti-oxidant, anti-bacterial, anti-inflammatory, immunomodulation and cytotoxic functions [3][4][5][6][7][8]. The main chemical conponents of Physalis alkekengi L. are physalins, neophysalins, alkaloids, polysaccharides and flavonoids, among which physalins are known as the most bioactive substances [9].…”

Section: Introductionmentioning

confidence: 99%

Physalis alkekengi var. franchetii extracts exert anti-tumour effects on non-small cell lung cancer and multiple myeloma by inhibiting STAT3 signalli

Zhu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Physalis alkekengi var. franchetii is an herb that possesses various ethnopharmacological applications. Herein, our current study focuses on the anti-tumour effect of the combination of various physalins, which are regarded as the most representative secondary metabolites from calyces of Physalis alkekengi var. franchetii.Methods: We mainly investigated the anti-tumour activity of the physalins extracted from Physalis alkekengi var. franchetii on both solid and haematologic cancers. The main cells used in this study were NCI-H1975 and U266 cells. The major assays used were the CCK8 assay, western blot analyses, an immunofluorescence assay, the Annexin V assay, and xenograft mouse model.Results: The results showed that physalins exhibited strong anti-tumoural effect on both non-small cell lung cancer (NSCLC) and multiple myeloma (MM) cells by suppressing the constitutive STAT3 activity and further inhibiting the downstream target genes expression of STAT3 signalling, resulting in the enhanced apoptosis of tumour cells. Moreover, physalins significantly reduced tumour growth in xenograft models of lung cancer.Conclusions: Collectively, these findings demonstrated that the physalins from Physalis alkekengi var. franchetii may potentially act as chemopreventive or chemotherapeutic agents for NSCLC and MM by inhibiting the STAT3 signalling pathway. It is promising that the present study served as a guide to further explore the precise mechanism of Physalis alkekengi var. franchetii in cancer treatment.

show abstract

Inhibitory effect of Physalis alkekengi L. var. franchetii extract and its chloroform fraction on LPS or LPS/IFN-γ-stimulated inflammatory response in peritoneal macrophages

Cited by 27 publications

References 23 publications

Bioactivity-guided fractionation of the triglyceride-lowering component and in vivo and in vitro evaluation of hypolipidemic effects of Calyx seu Fructus Physalis

Bioactivity-guided fractionation of the triglyceride-lowering component and in vivo and in vitro evaluation of hypolipidemic effects of Calyx seu Fructus Physalis

Physalis alkekengi Exhibits Antiobesity Effects in Mice with Potential of Inducing White Adipose Tissue Browning

Physalis alkekengi var. franchetii extracts exert anti-tumour effects on non-small cell lung cancer and multiple myeloma by inhibiting STAT3 signalli

Contact Info

Product

Resources

About