Inhibitory Effect of Oxalic Acid on Bacterial Spoilage of Raw Chilled Chicken

Anang, D.M.; Rusul, Gulam; Radu, Son; Bakar, Jamilah; Beuchat, Larry R.

doi:10.4315/0362-028x-69.8.1913

Cited by 25 publications

(12 citation statements)

References 32 publications

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“…Nine isolates of K. rhizophila were derived from sample KD, while the other 2 were identified from sample UG. Recently, the species K. rhizophila was identified as adventitious bacteria in chicken meat treated with oxalic acid ( Anang and others ), Korean salted and fermented seafood (Guan and others ), and milk and cheese samples (Alessandria and others ). On the other hand, K. varians was the most frequently isolated species of the Kocuria genus from several types of fermented sausage at a minor level (Papamanoli and others ; Martín and others ; García Fontán and others ) and is even used as a commercial starter culture in dry sausage processing.…”

Section: Resultsmentioning

confidence: 99%

Application of Different Molecular Techniques for Characterization of Catalase‐Positive Cocci Isolated from Sucuk

Kesmen

Yarimcam

Aslan

et al. 2014

Journal of Food Science

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This study was carried out for the characterization and discrimination of the indigenous Gram positive, catalase-positive cocci (GCC) population in sucuk, a traditional Turkish dry-fermented sausage. Sucuk samples, produced by the traditional method without starter culture were collected from 8 local producers in Kayseri/Turkey and a total of 116 GCC isolates were identified by using different molecular techniques. Two different molecular fingerprinting methods; namely, randomly amplified polymorphic DNA-PCR (RAPD-PCR) and repetitive extragenic palindrome-PCR (rep-PCR), were used for the clustering of isolates and identification at species level was carried out by full length sequencing of 16S rDNA. Combining the results obtained from molecular fingerprinting and 16S rDNA sequencing showed that the dominant GCC species isolated from the sucuk samples was Staphylococcus saprophyticus followed by Staphylococcus succinus and Staphylococcus equorum belonging to the Staphylococcus genus. Real-time PCR DNA melting curve analysis and high-resolution melting (HRM) analysis targeting the V1 + V3 regions of 16S rDNA were also applied for the discrimination of isolates belonging to different species. It was observed statistically different Tm values and species-specific HRM profiles for all except 2 species (S. saprophyticus and Staphylococcus xylosus) that have high 16S rDNA sequence similarity. The combination of rep-PCR and/or PCR-RAPD with 16S rRNA gene sequencing was an efficient approach for the characterization and identification of the GCC population in spontaneously fermented sucuk. On the other hand, intercalating dye assays were found to be a simple and very promising technique for the differentiation of the GCC population at species level.

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Section: Resultsmentioning

confidence: 99%

Application of Different Molecular Techniques for Characterization of Catalase‐Positive Cocci Isolated from Sucuk

Kesmen

Yarimcam

Aslan

et al. 2014

Journal of Food Science

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“…The selective cytotoxic activity of oxalic acid has been demonstrated on avian pathogenic Pseudomonas sp. (Anang et al 2006). According to these results, the hydroalcoholic extracts of B. nelumbiifolia could be useful to design industrial products for the biological control of the assayed bacterial species.…”

Section: Antimicrobial Activity Of Leaf Extracts On Phytopathogenic Bmentioning

confidence: 90%

Seasonal variation in phytochemicals and nutraceutical potential of Begonia nelumbiifolia consumed in Puebla, México

Villa‐Ruano¹,

Pacheco‐Hernández

Durán³

et al. 2017

J Food Sci Technol

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is a traditional edible plant consumed and commercialized in the northern highlands of Puebla, México. The present study reports the seasonal variation in proximate analysis as well as organic acids, carotenoids and flavonoids content in both leaves and stalks of this plant. The stalks contained low concentrations of protein (~3%), fiber (~1.5%) and nitrogen free extract (~0.26%) during the time of study. Both organs showed contents of oxalic acid (91-103 mg 100 g FW), ascorbic acid (50-65 mg 100 g FW), lutein (1-2.5 mg 100 g FW), β-carotene (1-3 mg 100 g FW), quercetin (1.3-2.7 mg 100 g DW) and rutin (0.5-1.7 mg 100 g DW). Antioxidant activity against DPPH was observed by the stalk extracts from 30% methanol (IC, 0.21-0.37 mg L), pure methanol (IC, 0.14-0.21 mg L) and hexane: acetone (IC, 0.77-1.21 mg L). In vitro anti-HMG-CoA reductase (IC, 0.07-0.36 mg L) and anti-alpha-glucosidase (IC, 0.28-0.43 mg L) activities were observed in extracts from the edible stalks from pure methanol and 30% methanol. The leaf extracts from 30% methanol inhibited the growth of pv. tabaci TBR2004 (MIC, 254 µg mL), pv. tomato DC3000 (MIC, 423 µg mL), pv. glycinea (MIC, 605 µg mL) and AB299158 (MIC, 162 µg mL). contained valuable phytochemicals associated to nutraceutical and biological properties. However, the consumption of the fresh stalks must be carefully considered because of the high oxalate content.

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“…It has been isolated from viz. cheese (8), chicken meat (9) and also healthy human GIT (1) across the globe, thus suggesting its wide adaption potential. Moreover, it has been important in industrial applications for antimicrobial susceptibility testing as standard quality control strain (1–3).…”

Section: Introductionmentioning

confidence: 99%

Functional and Comparative Genomics of Niche-Specific Adapted ActinomycetesKocuria rhizophilaStrain D2 Isolated from Healthy Human Gut

Ghattargi

Nimonkar

Sape³

et al. 2018

Preprint

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19Incidences of infection and occurrence of Kocuria rhizophila in human gut are prominent but 20 certainly no reports on the species ability to withstand human gastrointestinal dynamics. Kocuria 21 rhizophila strain D2 isolated from healthy human gut was comprehensively characterized. The 22 functional analysis revealed the ability to produce various gastric enzymes and sensitive to major 23 clinical antibiotics. It also exhibited tolerance to acidic pH and bile salts. Strain D2 displayed 24 bile-salt hydrolytic (BSH) activity, strong cell surface traits such as hydrophobicity, auto-25 aggregation capacity and adherence to human HT-29 cell line. Prominently, it showed no 26 hemolytic activity and was susceptible to the human serum. Exploration of the genome led to the 27 discovery of the genes for the above said properties and has ability to produce various essential 28 amino acids and vitamins. Further, comparative genomics have identified core, accessory and 29 unique genetic features. The core genome has given insights into the phylogeny while the 30 accessory and unique genes has led to the identification of niche specific genes. Bacteriophage, 31 virulence factors and biofilm formation genes were absent with this species. Housing CRISPR 32 and antibiotic resistance gene was strain specific. The integrated approach of functional, genomic 33 and comparative analysis denotes the niche specific adaption to gut dynamics of strain D2. 34Moreover the study has comprehensively characterized genome sequence of each strain to know 35 the genetic difference and intern recognize the effects of on phenotype and functionality 36 complexity. The evolutionary relationship among strains along and adaptation strategies has been 37 included in this study. 38Significance: Reports of Kocuria rhizophila isolation from various sources have been reported 40 but the few disease outbreaks in humans and fishes have been prominent, but no supportive 41 3 evidence about the survival ability of Kocuria spp. within human GIT. Here, we report the gut 42 adaption potential of K. rhizophila strain D2 by functional and genomic analysis. Further; 43 comparative genomics reveals this adaption to be strain specific (Gluten degradation). Genetic 44 difference, evolutionary relationship and adaptation strategies have been including in this study. 45 48 positive, non-hemolytic, non-endospore-forming, non-motile and can grow at different oxygen 49 levels (aerobic, facultative and anaerobic) (1, 2). Presence of galactosamine and glucosamine 50 (amino sugars) as the main component of the cell wall, differ them from other members of the 51class Actinobacteria (2). This genus is normally inhabitants of dust, soil, water and food and in 52 humans colonizes skin, mucosa, oropharynx and gastrointestinal tract (GIT) (3-5). 53K. rhizophila was isolated from the rhizoplane of the narrow-leaved cattail (Typha angustifolia). 54 in 1999 (6). The strain ATCC 9341 was reclassified as K. rhizophila from Micrococcus luteus 55 (7). It has been isolated from viz. c...

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Inhibitory Effect of Oxalic Acid on Bacterial Spoilage of Raw Chilled Chicken

Cited by 25 publications

References 32 publications

Application of Different Molecular Techniques for Characterization of Catalase‐Positive Cocci Isolated from Sucuk

Application of Different Molecular Techniques for Characterization of Catalase‐Positive Cocci Isolated from Sucuk

Seasonal variation in phytochemicals and nutraceutical potential of Begonia nelumbiifolia consumed in Puebla, México

Functional and Comparative Genomics of Niche-Specific Adapted ActinomycetesKocuria rhizophilaStrain D2 Isolated from Healthy Human Gut

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