Inhibitory Effect of Nivalenol, a Toxic Metabolite of Fusarium Nivale, on the Growth Cycle and Biopolymer Synthesis of Hela Cells

Mechanism of Inhibition of Eukaryotic Protein Synthesis by Trichothecene Fungal Toxins

“…This conclusion is in agreement with our precursor-incorporation data (Fig. 2) and with the previous work of others (3)(4)(5)11). Since disaggregation of polyribosomes induced by trichothecenes was inhibited by antibiotics such as cycloheximide or anisomycin, which inhibit the elongation of nascent polypeptides (16), we conclude that this process involved "runoff" of ribosomes from mRNA, i.e., the process involved protein synthesis.…”

“…In mammalian cells in culture, trichothecenes first inhibit protein synthesis and then DNA synthesis, whereas synthesis of RNA is not severely affected (3)(4)(5). Similar effects were obserVed in protozoan cultures (6).…”

Hypoglycemia in Mice Administered With Fusarenon-X

“…As in the case of cycloheximide treatment (Nakagawa and Nagai , 1971), gluconeogenesis may be blocked by inhibition of de nova synthesis of the key enzymes that is indispensable to the increase in enzymatic activities . As a noncytotoxic inhibitor of protein synthesis, cycloheximide induces complete disappearanec of mitotic figures in the crypt cells during 1.5-2.0 hr, by blocking G2 phase of the cell cycle (Verbin and Farber, 1967); while FX, by blocking all phases of the cell cycle (Ohtsubo, Yamada and Saito, 1968), inhibits the mitosis completely in 1 hr. X-ray irradiation is very similar to FX treatment in causing mitotic inhibition in the intestinal crypt cells, followed by cell degeneration and necrosis (Bloom and Fawcett, 1968).…”

Cytotoxic Effects of Scirpene Compounds, Fusarenon-X Produced by Fusarium Nivale, Dihydronivalenol and Dihydrofusarenon-X, on Hela Cells

“…In the previous report (Ohtsubo, Yamada and Saito, 1968) we elucidated that nivalenol, a toxic principle isolated from Fusarium nivale (Fn-2)-growing rice (Tatsuno et al, 1968), was a potent inhibitor of protein synthesis as well as DNA of HeLa cells. Subsequently, the chemical structure of nivalenol was identified by Tatsuno andothers (1968) as 3, 4, 7, 15-tetrahydroxyscirp-9-en-8-on (1968) (Fig.…”

“…Rats were about 10 times as susceptible to fusarenon-X as mice (Saito, Enomoto and Tatsuno , 1969). Furthermore, Ueno, Hosoya and Ishikawa (1969 b) Measurements of the incorporation rates of radioactive precursors into biopolymers were performed as previously described (Ohtsubo et al, 1968). 1969 b).…”