Inhibitory effect of natamycin against carrot white mold caused by Sclerotinia sclerotiorum

Abstract

This study was conducted to evaluate inhibitory effect of natamycin against three isolates of Sclerotinia sclerotiorum, the causal agent of carrot white mold in postharvest phase. The results showed that natamycin was able to markedly decrease radial growth and sclerogenesis of three isolates of the pathogen. In addition, natamycin significantly reduced myceliogenic and carpogenic germinations of sclerotia in three isolates. There was a clear positive correlation between natamycin concentration and efficacy against mycelial growth, sclerogenesis, and sclerotia germinations. Application of natamycin on carrot roots 2.5 h before inoculation with the pathogen markedly reduced white mold incidence. The treatment with natamycin enhanced relative conductivity of S. sclerotiorum mycelia over time compared with control. The concentration 2 mg L −1 increased the cell membrane permeability of mycelia more than the concentration 1 mg L −1 over 160 min. Moreover, an increase of carbohydrate leakage was observed in the pathogen mycelia incubated with natamycin. The content of methane dicarboxylic aldehyde (MDA) markedly increased at 5 h and the highest MDA content was observed after 20 h. Due to exposure to natamycin, a decrease percentage of catalase activity was observed in pathogen mycelia.

Natamycin as a safe food additive to control postharvest green mould and sour rot in citrus

“…The sensitivity of fruit fungal pathogens to natamycin has previously been shown in recent years. For instance, a concentration of 20 mg L −1 natamycin could inhibit mycelial growth of A. alternata and Fusarium semitectum (Cong et al, 2007); 2.5 mg L −1 natamycin completely inhibited mycelial growth and sclerogenesis of S. sclerotiorum (Ojaghian et al, 2020); and the minimum inhibitory concentrations of natamycin were 5.0 to 10.0 mg L −1 for mycelial growth and 0.7 to 1.0 mg L −1 for spore germination of Botrytis cinerea (Saito et al, 2020). The above results implicated that natamycin has strong antifungal action in vitro.…”

“…Moreover, natamycin‐resistant filamentous fungi have not been found yet, and fungi of resistance to other fungicides could still be restrained by natamycin (Saito et al, 2020). Previous studies revealed that natamycin displayed an inhibitory effect against Alternaria alternata , Colletotrichum acutatum and Sclerotinia sclerotiorum (Haack et al, 2018; Ojaghian et al, 2020; Wang et al, 2021). And natamycin also showed a suppression effect towards several citrus pathogens (Chen et al, 2021; Saito et al, 2020).…”

Field Application of Wuyiencin Against Sclerotinia Stem Rot in Soybean

“…However, due to longterm extensive application of chemical fungicides, pathogens have gradually evolved resistance, and pesticide residues can have negative impacts on human health and the environment (Huzar-Novakowiski et al, 2017). Therefore, biocontrol is an alternative to chemical control strategies, including the use of antagonistic bacteria, fungi, actinomycetes and virions, as well as their secondary metabolites and volatile organic compounds (Chacon-Orozco et al, 2020;Ojaghian et al, 2020b). Biological control agents (BCAs) are considered promising alternative management approaches that can address fungicide resistance while minimizing environmental pollution (Abdullah et al, 2008).…”