Inhibitory Effect of Melanoma Differentiation Associated Gene-7/Interleukin-24 on Invasion In Vitro of Human Melanoma Cancer Cells

Lin, Biwen; Jiao, Ze-long; Fan, Jianfeng; Peng, Liang; Li, Lei; Zhao, Zhiyun; Ding, Xiangyu; Li, Hengjin

doi:10.3346/jkms.2013.28.6.833

Cited by 10 publications

(11 citation statements)

References 25 publications

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“…Furthermore, activated PI3K/AKT signaling are involved tumor cell invasion and oncogenesis and this signaling also involved in melanoma cells . More interesting is that the inhibition of the PI3k/Akt pathway led to decrease the invasion of melanoma cells . Results from Figure B also showed that chrysin suppressed the expression of PI3K, p‐AKT(Thr308), p‐AKT(Ser473), and PKC in A375.S2 cells.…”

Section: Discussionmentioning

confidence: 85%

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Chrysin inhibit human melanoma A375.S2 cell migration and invasion via affecting MAPK signaling and NF‐κB signaling pathway in vitro

Chen

Jiang

Kuo

et al. 2018

Environmental Toxicology

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Numerous evidences have shown that chrysin induced cytotoxic effects via induced cell cycle arrest and induction of cell apoptosis in human cancer cell lines, however, no information showed that chrysin inhibited skin cancer cell migration and invasion. In this study, we investigated anti-metastasis mechanisms of chrysin in human melanoma cancer A375.S2 cells in vitro.Under sub-lethal concentrations of chrysin (0, 5, 10, and 15 μM) which inhibits cell mobility, migration and invasion of A375.S2 cells that were assayed by wound healing and Transwell filter. That chrysin inhibited MMP-2 activity in A375.S2 cells was investigated by gelatin zymography assay. Western blotting was used to examine protein expression and results indicated that chrysin inhibited the expression of GRB2, SOS-1, PKC, p-AKT (Thr308), NF-κBp65, and NF-κBp50 at 24 and 48 hours treatment, but only at 10-15 μM of chrysin decreased Ras, PI3K, p-c-Jun, and Snail only at 48 hours treatment and only decrease p-AKT(Ser473) at 24 hours treatment. Furthermore, chrysin (5-15 μM) decreased the expression of uPA, N-cadherin and MMP-1 at 24 and 48 hours treatment but only decreased MMP-2 and VEGF at 48 hours treatment at 10-15 μM and 5-15 μM of chrysin, respectively, however, increased E-cadherin at 5-15 μM treatment. Results of confocal laser microscopy systems indicated that chrysin inhibited expression of NF-κBp65 in A375.S2 cells. Based on these observations, we suggest that chrysin can be used in anti-metastasis of human melanoma cells in the future. K E Y W O R D SA375.S2 cells, chrysin, invasion, metastasis, migration

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Section: Discussionmentioning

confidence: 85%

“…PI3K/Akt is one of the major pathway for tumor invasion, plays a role in MMPs for u‐PA gene regulation, cell survival and cell invasion . AKT activation induce cancer cells invasion and metastasis by stimulates secretions of MMPs …”

Section: Discussionmentioning

confidence: 99%

Chrysin inhibit human melanoma A375.S2 cell migration and invasion via affecting MAPK signaling and NF‐κB signaling pathway in vitro

Chen

Jiang

Kuo

et al. 2018

Environmental Toxicology

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“…Figure A indicated that Ras, p‐AKT (Thr308) and p‐AKT (Ser473) were reduced in TET treatment at 24 and 48 hours in GBM 8401 cells. The down‐regulation of the PI3k/Akt pathway were associated with the reducing the invasion of melanoma cells . AKT, downstream of PI3K have been reported to be suppressed by the transcription of the E‐cadherin gene .…”

Section: Discussionmentioning

confidence: 99%

Tetrandrine inhibits human brain glioblastoma multiforme GBM 8401 cancer cell migration and invasion in vitro

Jiang

Cheng

Kuo

et al. 2018

Environmental Toxicology

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Tetrandrine (TET) has been reported to induce anti-cancer activity in many human cancer cells and also to inhibit cancer cell migration and invasion. However, there are no reports to show TET inhibits cell migration and invasion in human brain glioblastoma multiforme GBM 8401 cells. In this study, we investigated the anti-metastasis effects of TET on GBM 8401 cells in vitro. Under sub-lethal concentrations (from 1, 5 up to 10 μM), TET significantly inhibited cell mobility, migration and invasion of GBM 8401 cells that were assayed by wound healing and Transwell assays. Gelatin zymography assay showed that TET inhibited MMP-2 activity in GBM 8401 cells. Western blotting results indicated that TET inhibited several key metastasis-related proteins, such as p-EGFR (Tyr1068) , SOS-1, GRB2, Ras, p-AKT (Ser473) and p-AKT (Thr308) , NF-κB-p65, Snail, E-cadherin, N-cadherin, NF-κB, MMP-2 and MMP-9 that were significant reduction at 24 and 48 hours treatment by TET. TET reduced MAPK signaling associated proteins such as p-JNK1/2 and p-c-Jun in GBM 8401 cells. The electrophoretic mobility shift (EMSA) assay was used to investigate NF-κB and DNA binding was reduced by TET in a dose-dependently. Based on these findings, we suggested that TET could be used in anti-metastasis of human brain glioblastoma multiforme GBM 8401 cells in the future. K E Y W O R D S GBM 8401 cells, migration and invasion, MMP-2, NF-κB, Tetrandrine (TET) 1 | INTRODUCTION Malignant glioblastoma multiforme (GBM) are the most common and aggressive malignant primary brain tumor in humans and can be a fatal tumor because of difficulties in treating the related metastasis due to the high proliferation rate and invasiveness. 1-5 Currently, the treatment of GBM is the local irradiation, and conventional chemotherapy with temozolomide (TMZ) or surgical resection in combination with radiotherapy and chemotherapy but the median survival rate is only 8-15 months. 4-6 About 20% of patients after treated with TMZ have shown the clinical toxicity. 7 Due to the side effects of TMZ chemotherapy that leading to the limited efficiency. Thus, bases on the Yun-Lian Lin and Jing-Gung Chung contributed equally to this study.

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“…The MAPK pathway regulated various cellular activities such as proliferation, invasion, metastasis, and death (52). It was suggested that agents to inhibit the MAPK pathway might lead to prevent cancer angiogenesis, proliferation, invasion, and metastasis including melanoma (49,53). Results from western blotting indicated that PEITC and BITC treatment at 24 and 48 h significantly reduced the expression of p-ERK1/2, p-p38 and p-JNK1/2 when compared to control (Fig.…”

Section: Discussionmentioning

confidence: 90%

Benzyl isothiocyanate and phenethyl isothiocyanate inhibit murine melanoma B16F10 cell migration and invasion in vitro

Lai

Hsiao

Yang

et al. 2017

International Journal of Oncology

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Benzyl isothiocyanate (BITC), and phenethyl isothiocyanate (PEITC) have been demonstrated to induce anticancer function in many human cancer cells and also inhibit cancer cell migration and invasion. However, there are no studies that show BITC and PEITC to inhibit cell migration and invasion in mouse melanoma B16F10 cells. In this study, we investigated anti-metastasis effects of BITC and PEITC in melanoma cancer cells in vitro. Under sub-lethal concentrations (from 1, 2.5 up to 5 µM), BITC and PEITC significantly inhibited cell mobility, migration and invasion nature of B16F10 cells. Gelatin zymography assay also showed that BITC and PEITC inhibited matrix metalloproteinase-2 (MMP-2) activity in B16F10 cells. PEITC reduced MAPK signaling associated proteins such as p-ERK1/2, p-p38 and p-JNK1/2 but BITC increased those MAPK signaling associated proteins. BITC and PEITC both suppressed the expression of RhoA, Ras, and SOS-1, however, PEITC increased FAK and GRB2 but BITC increased FAK at 48 h. Furthermore, PEITC decreased the expression of MMP-2 and tissue inhibitors of matrix metalloproteinases (TIMP) but BITC increased them. PEITC inhibited NF-κB protein levels and DNA binding which was confirmed by electrophoretic mobility shift (EMSA) assay. Based on these observations, we suggest that BITC and PEITC can be used in anti-metastasis of melanoma cells in the future.

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Inhibitory Effect of Melanoma Differentiation Associated Gene-7/Interleukin-24 on Invasion In Vitro of Human Melanoma Cancer Cells

Cited by 10 publications

References 25 publications

Chrysin inhibit human melanoma A375.S2 cell migration and invasion via affecting MAPK signaling and NF‐κB signaling pathway in vitro

Chrysin inhibit human melanoma A375.S2 cell migration and invasion via affecting MAPK signaling and NF‐κB signaling pathway in vitro

Tetrandrine inhibits human brain glioblastoma multiforme GBM 8401 cancer cell migration and invasion in vitro

Benzyl isothiocyanate and phenethyl isothiocyanate inhibit murine melanoma B16F10 cell migration and invasion in vitro

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