Inhibitory Effect of Magnolol and Honokiol from<i>Magnolia obovata</i>on Human Fibrosarcoma HT-1080 Invasiveness<i>in vitro</i>

Nagase, Hisamitsu; Ikeda, Kazuhiko; Sakai, Yoshimichi

doi:10.1055/s-2001-18345

Cited by 73 publications

(53 citation statements)

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“…The results demonstrated that honokiol had a similar effect to adriamycin in prolongation of lifespan of tumor-bearing nude mice. Previous studies suggest that growth inhibition by honokiol resulted from the induction of apoptosis in several cell lines [7][8][9][10][11] . A further study reported that in human squamous lung cancer cells, honokiol induced apoptosis by down-regulating Bcl-X L and sequentially activating Caspase cascade [10] .…”

Section: Prolongation Of Life-span In Nude Mice Bearing Rko Solid Tumormentioning

confidence: 99%

“…With its major active constituent extracted from the bark of Houpu, honokiol has been found having a variety of pharmacological effects, such as anti-inflammatory [2] , antithrombotic [3] , anti-arrhythmic [4] , antioxidative [5] and anxiolytic effects [6] . Recently, honokiol has been reported to exhibit a potent cytotoxicity by inducing cell apoptosis in rat and human leukemia cells [7,8] , human fibrosarcoma cells [9] , human squamous lung cancer CH27 cells [10] and human SVR angiosarcoma cells [11] , yet there has been no report on honokiol in the treatment of human colorectal carcinoma.…”

Section: Introductionmentioning

confidence: 99%

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Honokiol: A potent chemotherapy candidate for human colorectal carcinoma

Chen

Wang²,

Wu³

et al. 2004

WJG

112

100

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AIM:To investigate the anticancer activity of Honokiol on RKO, a human colorectal carcinoma cell line in vitro and in vivo, and to evaluate its possible use in clinic. METHODS:In vitro anticancer activity of honokiol was demonstrated by its induction of apoptosis in tumor cells. We analyzed cell proliferation with MTT assay, cell cycle with flow cytosmeter, DNA fragment with electrophoresis on agarose gels. To test the mechanism of honokiol-induced apoptosis, Western blotting was used to investigate the factors involved in this process. The pharmacokinetics study of honokiol was tested by high phase liquid chromatography. In in vivo study, Balb/c nude mice were incubated with RKO cells. Honokiol was injected intraperitoneally every other day into tumor bearing Balb/c nude mice. RESULTS:Our results showed that honokiol induced apoptosis of RKO cells in a time-and dose-dependent manner. At 5-10 ug/mL for 48 h, honokiol induced apoptosis through activating Caspase cascades. Pharmacokinetics study demonstrated that, honokiol could be absorbed quickly by intraperitoneal injection, and maintained in plasma for more than 10 h. In nude mice bearing RKO-incubated tumor, honokiol displayed anticancer activity by inhibiting tumor growth and prolonging the lifespan of tumor bearing mice. CONCLUSION:With its few toxicity to normal cells and potent anticancer activity in vitro and in vivo, honokiol might be a potential chemotherapy candidate in treating human colorectal carcinoma.Chen F, Wang T, Wu YF, Gu Y, Xu XL, Zheng S, Hu X. Honokiol: A potent chemotherapy candidate for human colorectal carcinoma.

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Section: Prolongation Of Life-span In Nude Mice Bearing Rko Solid Tumormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Honokiol: A potent chemotherapy candidate for human colorectal carcinoma

Chen

Wang²,

Wu³

et al. 2004

WJG

112

100

View full text Add to dashboard Cite

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“…Recent studies have provided experimental evidence to document anticancer effects of honokiol (5 -14). For example, the ethanol extract of Magnolia obovata and honokiol inhibited the migration of HT-1080 human fibrosarcoma cells (6). Honokiol treatment caused apoptotic cell death in CH27 human squamous lung cancer cell line in association with the up-regulation of Bad and down-regulation of Bcl-xL protein expression, release of cytochrome c from the mitochondria to the cytosol, and activation of caspases (7).…”

Section: Introductionmentioning

confidence: 99%

Honokiol causes G0-G1 phase cell cycle arrest in human prostate cancer cells in association with suppression of retinoblastoma protein level/phosphorylation and inhibition of E2F1 transcriptional activity

Hahm

Singh

2007

Molecular Cancer Therapeutics

104

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The present study was undertaken to gain insights into the mechanism of cell cycle arrest caused by honokiol, a constituent of oriental herb Magnolia officinalis. The honokiol treatment decreased the viability of PC-3 and LNCaP human prostate cancer cells in a concentrationand time-dependent manner, which correlated with G 0 -G 1 phase cell cycle arrest. The honokiol-mediated cell cycle arrest was associated with a decrease in protein levels of cyclin D1, cyclin-dependent kinase 4 (Cdk4), Cdk6, and/or cyclin E and suppression of complex formation between cyclin D1 and Cdk4 as revealed by immunoprecipitation using anti -cyclin D1 antibody followed by immunoblotting for Cdk4 protein. The honokiol-treated PC-3 and LNCaP cells exhibited a marked decrease in the levels of total and phosphorylated retinoblastoma protein (Rb), which correlated with the suppression of transcriptional activity of E2F1. Exposure of PC-3 and LNCaP cells to honokiol resulted in the induction of p21 (PC-3 and LNCaP) and p53 protein expression (LNCaP). However, small interfering RNA (siRNA) -mediated knockdown of either p21 (PC-3 and LNCaP) or p53 (LNCaP) protein failed to confer any protection against honokiol-induced cell cycle arrest. The honokiol treatment caused the generation of reactive oxygen species (ROS), and the cell cycle arrest caused by honokiol was partially but significantly attenuated in the presence of antioxidant N-acetylcysteine. In conclusion, the present study reveals that the honokiol-mediated G 0 -G 1 phase cell cycle arrest in human prostate cancer cells is associated with the suppression of protein level/phosphorylation of Rb leading to inhibition of transcriptional activity of E2F1. [Mol Cancer Ther 2007; 6(10):2686 -95]

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“…Previous studies suggest that growth inhibition by HNK resulted from the induction of apoptosis in several cell lines [9][10][27][28][29]. A further study reported that HNK induces a necrotic cell death through the mitochondrial permeability transition pore [30].…”

Section: Discussionmentioning

confidence: 99%