Dietary cis,trans-18 : 2 isomers impair desaturation and elongation of linoleic acid (D9cis,12cis-18 : 2), but little is known of their proportional partitioning between accumulation and oxidation. The present study was therefore designed to assess the accumulation and apparent oxidation of cis,trans-18 : 2 isomers compared with that of trans-18 : 1 isomers and D9cis,12cis-18 : 2 in rats. Accumulation is defined as whole-body increase in a fatty acid during a given period (i.e. final body content2initial body content). The apparent oxidation (disappearance) is defined as whole-body utilization of a fatty acid relative to its intake for a given period (intake2excretion2accumulation2longer-chain products)/intakeÂ100). The animals were fed on a diet containing 15 % (w/w) partially hydrogenated rapeseed oil with 1 : 72 % energy as cis,trans-18 : 2 isomers and varying amounts of D9cis,12cis-18 : 2. The apparent oxidation of total cis,trans-18 : 2 isomers (72 -76 % dietary intake) was greater than that of D9cis,12cis-18 : 2 (38 -51 % dietary intake) but it was similar to that of total trans-18 : 1 isomers (78 -82 % dietary intake). Among the four isomers, the apparent oxidation of D9trans,12-trans-18 : 2 was greater than that of the other isomers including D9trans,12cis-18 : 2, D9cis,12trans-18 : 2 and D9cis,13trans-18 : 2. Accumulation of D5cis,8cis,11cis,15trans-20 : 4 and D5cis,8cis,11cis,14trans-20 : 4 derived from chain-elongation and desaturation of D9cis,13trans-18 : 2 and D9cis,12trans-18 : 2 was decreased when the dietary D9cis,12cis-18 : 2 supply was increased.