Inhibitory effect of Kangjia Pill (抗甲丸) on thyrocyte proliferation in rat goiter model

Han, Yong; Zhou, Jing; Yu, Shanshan; Cui, Bin; Zhang, Haiqing; Gao, Ling; Zhao, Jiajun

doi:10.1007/s11655-009-0284-8

Cited by 1 publication

(2 citation statements)

References 28 publications

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“…To confirm the inhibition of GD by diosgenin in vivo , we constructed a rat model of GD goiter. Based on previous research ( Han et al, 2009 ; Cai et al, 2014 ) and following the best practice in pharmacological research ( Heinrich et al, 2020 ), the safe, effective and non-toxic concentration of MMI and diosgenin were selected. The relative thyroid weight of the MMI group was significantly higher than that of the Norm group.…”

Section: Resultsmentioning

confidence: 99%

“…One week later, rats were treated with 20 mg kg −1 d −1 or 80 mg kg −1 d −1 diosgenin by intraperitoneal injection. Correspondingly, the Norm and MMI groups were injected with the same amount of solvent (4% Tween-80) for 3 weeks ( Han et al, 2009 ). All animals were anesthetized, and the thyroid glands were collected and fixed in 4% paraformaldehyde for hematoxylin-eosin (H&E) and immunohistochemistry staining.…”

Section: Methodsmentioning

confidence: 99%

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Diosgenin From Dioscorea Nipponica Rhizoma Against Graves’ Disease—On Network Pharmacology and Experimental Evaluation

Xin

Cheng

et al. 2022

Front. Pharmacol.

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Dioscorea nipponica rhizoma (DNR) is commonly used for the cure of hyperthyroidism resulting from Graves’ disease (GD) or thyroid nodules. However, its therapeutic mechanism remains unclear. This study aimed to utilize network pharmacology integrated molecular docking and experimental verification to reveal the potential pharmacological mechanism of DNR against GD. First, the active componds of DNR were collected from the HERB database and a literature search was conducted. Then, according to multisource database, the predicted genes of DNR and GD were collected to generate networks. The analysis of protein–protein interaction and GO enrichment and KEGG pathway were employed to discover main mechanisms associated with therapeutic targets. Moreover, molecular docking simulation was applied in order to verify the interactions between the drug and target. Finally, our experiments validated the ameliorated effects of diosgenin, the main component of DNR, in terms of phosphorylation deactivation in IGF-1R, which in turn inhibited the phosphorylation and activation of PI3K-AKT and Rap1-MEK signaling pathways, promoting cell apoptosis and GD remission. Our present study provided a foundation for further investigation of the in-depth mechanisms of diosgenin in GD and will provide new scientific evidence for clinical application.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Diosgenin From Dioscorea Nipponica Rhizoma Against Graves’ Disease—On Network Pharmacology and Experimental Evaluation

Xin

Cheng

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

Inhibitory effect of Kangjia Pill (抗甲丸) on thyrocyte proliferation in rat goiter model

Abstract: KJP played a therapeutic role via inhibiting cell proliferation in the rat goitrous glands.

Cited by 1 publication

References 28 publications

Diosgenin From Dioscorea Nipponica Rhizoma Against Graves’ Disease—On Network Pharmacology and Experimental Evaluation

Diosgenin From Dioscorea Nipponica Rhizoma Against Graves’ Disease—On Network Pharmacology and Experimental Evaluation

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