Inhibitory effect of K+ and Ca2+ concentrations, pH, and osmolality of activation solution on motility of shabut (Barbus grypus Heckel 1843) spermatozoa

Öğretmen, Fatih; Gölbaşı, Selami; İnanan, Burak Evren

doi:10.3906/vet-1308-9

Cited by 13 publications

(6 citation statements)

References 24 publications

(36 reference statements)

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“…In freshwater fish species, a 6‐mM potassium (K + ) solution was found to have an inhibitory action on sperm motility and above 12 mM caused a complete cessation of sperm movement while Na + solution inhibited sperm motility of European burbot, Lota lota L. at concentrations over 240 mM (Dziewulska & Pilarska, 2018) and the highest percentage of sperm motility of shabut, Barbus grypus , was observed in solution containing 5 mM K + without the addition of Ca 2+ ion (Ogretmen et al., 2014). In marine fish species, the effects of ionic strength on motility activation of sperm have received much less attention.…”

Section: Discussionmentioning

confidence: 99%

“…at concentrations over 240 mM (Dziewulska & Pilarska, 2018) and the highest percentage of sperm motility of shabut, Barbus grypus, was observed in solution containing 5 mM K + without the addition of Ca 2+ ion (Ogretmen et al, 2014). In marine fish species, the effects of ionic strength on motility activation of sperm have received much less attention.…”

Section: Effects Of Ionic and Non-ionic Concentrations On Sperm Activationmentioning

confidence: 99%

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Chilled storage of Asian seabass,Lates calcariferBloch semen: Effects of ions, extenders and storage periods on sperm quality and fertilization ability

2021

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Asian seabass (Lates calcarifer, Bloch), a euryhaline fish reproduced in seawater is one of the most important aquaculture species throughout Southeast Asia and Australia due to acceptable flavor and high market demand. Annual production of L. calcarifer in Thailand was 2,503 tons in 1994 and increased to 20,454 tons, equivalent a market value of nearly 90 million US$ in 2017 (Fisheries Statistics of Thailand, 2019). This indicates an increase in a market need of L. calcarifer seed stocks for domestic and international trades. One of the major challenges to manage the breeding program of L. calcarifer held in hatcheries is the uncertainty from year to year in the number of functional male broodstocks due to the natural sex inversion from male to female. Because of a cohort reproductive behavior, several L. calcarifer males are mated with one or two females (at a ratio of 4:1, a common practice in the hatcheries of Thailand), which consequently generates a back-up supply of highly desired male broodstocks. Furthermore, stress derived from the handling was deteriorative to the quality of expressible semen of male broodstocks (Palmer et al., 1993).Chilled storage of fish semen is a simple protocol that extends sperm life from a few hours to several weeks with maintaining

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Section: Discussionmentioning

confidence: 99%

Section: Effects Of Ionic and Non-ionic Concentrations On Sperm Activationmentioning

confidence: 99%

Chilled storage of Asian seabass,Lates calcariferBloch semen: Effects of ions, extenders and storage periods on sperm quality and fertilization ability

2021

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“…The percentage of sperm motility was estimated as the cell performing progressive forward movement while the duration of motility was determined as the time until forward movement stops. Determining the percentage of sperm motility was assessed using an arbitrary scale with 10% interval increments in which non motile represents 0% [14,[37][38][39][40].…”

Section: Methodsmentioning

confidence: 99%

In Vitro Effect of Zinc: Evaluation of the Sperm Quality of Endangered Trout Salmo Coruhensis and Rainbow Trout Oncorhynchus Mykiss and Fertilizing Capacity

Kutluyer¹

2017

Int J Aquac Fish Sci

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This study was intended to reveal the usefulness of Zinc in endangered trout Salmo coruhensis and rainbow trout Oncorhynchus mykiss sperm. Spermatozoa were activated in sperm motility-activation solutions (NaCl, 0.3%; NaHCO 3 , 1%) containing the Zinc [Control (0), 0.5, 1, 2, 3, 4 and 5 mM]. The percentage and duration of motility, fertility and hatching rate were determined in sperm samples. Our results indicated that the percentage and duration of motility, fertility and hatching rate increased when activation solution containing NaHCO 3 was supplemented with 1 mM Zinc in rainbow trout (O. mykiss). On the contrary, motility rate in endangered trout (S. coruhensis) was increased by Zinc compared to control group. The percentage and duration of motility, fertility and hatching rate were affected by increasing concentrations of Zinc in endangered trout (S. coruhensis) and rainbow trout (O. mykiss) (p<0.05). In conclusion, sperm quality was affected by quantitative changes different concentrations of Zinc and the best results were obtained from a concentration of 1 mM for rainbow trout.

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“…Digested solutions were diluted with deionized water until adjusted to 10 mL. After dilution, they were analyzed for Na, K, Ca, Mg, Al, B, Cu, Li, and Zn (Massanyi et al, 2008;Özgür et al, 2015;Slivkova et al, 2009). Metal concentrations were determined by inductively coupled plasma optical emission spectrometry (ICP-OES VARIAN 725-ES, with CCD detector).…”

Section: The Analysis Of Semen Seminal Plasma Ionsmentioning

confidence: 99%

The relationship between semen seminal plasma ions and sperm cell velocities of wild-caught longspine scraper, <i>Capoeta trutta</i>

2019

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In this study, semen seminal plasma contents and the motility of sperm cells were determined in Capoeta trutta via a computer-assisted sperm analysis system. In addition, we evaluated the relationship between semen seminal plasma ions and the velocities of sperm cells. Although the predominant ions were K (206.84±20.61 mg L-normal1) and Na (128.06±23.82 mg L-normal1) in the semen seminal plasma, Ca (14.05±4.13 mg L-normal1) and Mg (3.35±0.44 mg L-normal1) were not predominate according to our results. However, partially strong relationships between the curvilinear velocity value (VCL) and K (Rnormal2=0.67; p<0.05) were found, while it was moderate with Mg (Rnormal2=0.48; p<0.05). There was a weak relationship with Na (Rnormal2=0.17; p<0.05) and Ca (Rnormal2=0.34; p<0.05). In our results, while the trace metals were determined as Zn > Al > B > Li > Cu in semen seminal plasma, they are not correlated with sperm cell velocities. Finally, we hope that the present information on the motility parameters of Capoeta trutta in this paper will eventually help artificial insemination in reproduction practices.

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Inhibitory effect of K+ and Ca2+ concentrations, pH, and osmolality of activation solution on motility of shabut (Barbus grypus Heckel 1843) spermatozoa

Cited by 13 publications

References 24 publications

Chilled storage of Asian seabass,Lates calcariferBloch semen: Effects of ions, extenders and storage periods on sperm quality and fertilization ability

Chilled storage of Asian seabass,Lates calcariferBloch semen: Effects of ions, extenders and storage periods on sperm quality and fertilization ability

In Vitro Effect of Zinc: Evaluation of the Sperm Quality of Endangered Trout Salmo Coruhensis and Rainbow Trout Oncorhynchus Mykiss and Fertilizing Capacity

The relationship between semen seminal plasma ions and sperm cell velocities of wild-caught longspine scraper, <i>Capoeta trutta</i>

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Inhibitory effect of K+ and Ca2+ concentrations, pH, and osmolality of activation solution on motility of shabut (Barbus grypus Heckel 1843) spermatozoa

Cited by 13 publications

References 24 publications

Chilled storage of Asian seabass,Lates calcariferBloch semen: Effects of ions, extenders and storage periods on sperm quality and fertilization ability

Chilled storage of Asian seabass,Lates calcariferBloch semen: Effects of ions, extenders and storage periods on sperm quality and fertilization ability

In Vitro Effect of Zinc: Evaluation of the Sperm Quality of Endangered Trout Salmo Coruhensis and Rainbow Trout Oncorhynchus Mykiss and Fertilizing Capacity

The relationship between semen seminal plasma ions and sperm cell velocities of wild-caught longspine scraper, &lt;i&gt;Capoeta trutta&lt;/i&gt;

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The relationship between semen seminal plasma ions and sperm cell velocities of wild-caught longspine scraper, <i>Capoeta trutta</i>