Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrest

Abstract

Although various parts of J. curcas (Jatropha curcas L., Euphorbiaceae) have long been used as traditional folk medicines for their antiviral, analgesic, and/or antidotal efficacies, we are the first to investigate the role of anti-carcinogenicity of isoamericanol A (IAA) from the seed extract. Our results showed that IAA is capable of inhibiting cell proliferation in a dose-dependent manner on the human cancer cell lines of MCF-7, MDA-MB231, HuH-7, and HeLa. Flow cytometry analysis showed IAA significantly induces cell cycle arrest at G2/M on MCF-7 cells. At both protein and mRNA levels examined by western blot and real-time PCR, the results revealed increased expression of BTG2 (B-cell translocation gene 2), p21 (p21WAF1/CIPI), and GADD45A (growth arrest and DNA-damage-inducible, alpha) after IAA treatment, but inversed expression in CDK1 (cyclin-dependent kinase 1) and cyclins B1 and B2. All these effects contribute to G2/M cell cycle arrest. Furthermore, these results coincide with the changes in molecular expressions determined by DNA-microarray analysis. Our findings indicate that IAA has an inhibitory effect on cell proliferation of MCF-7 through cell cycle arrest, giving it great potential as a future therapeutic reagent for cancers.

Composition and Anti-inflammatory Activity of Essential Oil of Jatropha Curcas

“…J. curcas shell is rich in phenolic compounds with high antioxidant activity [10] and fatty acids [11]. The constituents of J. curcas include curcin, with hemagglutinating activity [12], phorbol esters [13], lathyranlactone, jatrocurcasenones A-E and jatrophodiones B-E with cytotoxic activity [14], curcusones A-E, jatrogrossidione and 2-epi-jatrogrossidione, reported for their cytotoxity [15], isoamericanol A, which has an inhibitory effect on cell proliferation of MCF-7 [16], curcacycline A, which displays a moderate inhibition of proliferation of human T-cells [17], and curcacycline B, known to enhance the rotamase activity of human cyclophilin B [18]. Phytol (47.42%) in the leaf, 1butoxyperethyl-homotetrasilsesquioxane (36.11%) in the petiole, 7benzyl-8-(methylthio)theophyllin (12.53%) in the stem, as well as -gurjunene (13.99 %), β-elemol (13.45%) and guaiene (13.13%) in the root, were major components present in the essential oil [19].…”

Chemical analysis of callus extracts from toxic and non-toxic varieties of Jatropha curcas L.

“…Jatropha curcas L. (Euphorbiaceae) is a multipurpose plant native to Mesoamerica, and it is important because of its usefulness as raw material in biofuels production ( Salvador-Figueroa et al, 2015 ) as well as in veterinary and human traditional medicine ( Zhang et al, 2017 ). Several compounds with different biological activities have been isolated from different species of Jatropha ( Ferreira-Rodrigues et al., 2016 ; Katagi et al, 2016 ). The identification of biologically active compounds extracted from different organs of this plant has been reported ( Prasad, Izam & Khan, 2012 ; Sharma, Dhamija & Parashar, 2012 ).…”

A catechol-type lignan and neolignans are specifically present in the seed coat of tung trees

“…The lignans, which may have been generated from the oxidative coupling of caffeyl alcohol or caffeic acid, have shown interesting physiological activities. Isoamericanol A has been reported to show neurotrophic activity on rat cells [7], antimelanogenic activity on Melan-a cells [8], anticancer activity on human breast cancer cell lines [9][10][11], as well as plant growth inhibitory activity [12]. Furthermore, isoamericanin A was isolated as a prostaglandin I 2 inducer [13], and (+)-3,3′-bisdemethylpinoresinol has been reported as a metabolite of (+)-sesamin in rat liver homogenate [14,15].…”