Inhibitory Effect of Rosae Multiflorae Fructus Extracts on the Receptor Activator of NF-κB Ligand-Induced Osteoclastogenesis through Modulation of P38- and Ca2+-Mediated Nuclear Factor of Activated T-Cells Cytoplasmic 1 Expression

Park, Keun Ha; Gu, Dong Ryun; Kim, Min Seuk; Lee, Seoung Hoon

doi:10.11005/jbm.2020.27.1.53

Cited by 4 publications

(5 citation statements)

References 32 publications

(47 reference statements)

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“…Differences in the cell culture model, tested flavonoids, levels, and exposure time, evaluated parameters, and, importantly, the nature of the functionalized biomaterial, hinder the establishment of patterns. Nevertheless, reported information strongly supports the positive role of flavonoids in bone formation [ 68 ]. However, in the present work, with the used experimental protocol, the relative contribution of the particles physicochemical profile or the presence of the RH extract is not known.…”

Section: Discussionmentioning

confidence: 71%

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Rosehip Extract-Functionalized Magnesium Hydroxide Nanoparticles and Its Effect on Osteoblastic and Osteoclastic Cells

et al. 2021

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Considering the role of magnesium in bone metabolism and the increasing relevance of plant-mediated green-synthesis, this work compares the bone cytocompatibility of magnesium hydroxide nanoparticles (NPs) produced by using pure water, Mg(OH)2, or a rosehip (RH) aqueous extract, Mg(OH)2RH. The NPs were evaluated for dose- and time-dependent effects on human osteoblastic and osteoclastic response, due to the direct involvement of the two cell types in bone metabolism. Mg(OH)2 NPs presented nanoplatelet-like morphology (mean diameter ~90 nm) and a crystalline structure (XRD analysis); the RH-mediated synthesis yielded smaller rounded particles (mean diameter <10 nm) with decreased crystallinity. On the ATR–FTIR spectra, both NPs presented the characteristic Mg-OH peaks; Mg(OH)2RH exhibited additional vibration bands associated with the presence of phytochemicals. On osteoblastic cells, NPs did not affect cell growth and morphology but significantly increased alkaline phosphatase (ALP) activity; on osteoclastic cells, particles had little effect in protein content, tartrate-resistant acid phosphatase (TRAP) activity, percentage of multinucleated cells, and cell area. However, compared with Mg(OH)2, Mg(OH)2RH increased osteoblastic differentiation by inducing ALP activity and promoting the expression of Runx2, SP7, Col1a1, and ALP, and had a negative effect on the expression of the osteoclastic genes NFATC1, CA2, and CTSK. These observations suggest the potential usefulness of Mg(OH)2RH NPs in bone regeneration.

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Section: Discussionmentioning

confidence: 71%

“…RH contains high levels of flavonoids, a major subclass of polyphenols that have been shown to promote osteoblast differentiation, which ultimately results in bone formation [ 67 ]. Some of these compounds appear to inhibit osteoclastogenesis [ 68 ]. Still, there are also studies reporting different effects in these processes.…”

Section: Discussionmentioning

confidence: 99%

Rosehip Extract-Functionalized Magnesium Hydroxide Nanoparticles and Its Effect on Osteoblastic and Osteoclastic Cells

et al. 2021

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“…Rosa multiflora is divided into RMF and Rosa multiflora Thunb (RM). Several recent studies have shown that RM effectively improves sleep quality and acts as an antioxidant and anti-inflammatory agent [ 17 , 18 ]. In addition, RMF exhibits anti-atopic, anti-allergic, and anti-inflammatory effects similar to those of Donguibogam in Korean Oriental medicine [ 19 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, studies have reported that natural products, such as herbs, botanical extracts, and dietary supplements, have been studied for their potential effects on obesity-related factors [ 13 ]. In this study, we attempted to analyze Rosae multiflorae fructus (RMF), a representative natural product for treating circulatory diseases, as specified in Donguibogam, an important book of Korean medicine [ 14 ]. However, the role of RMF in liver disease remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Rosae multiflorae fructus regulates the lipogenesis in high-fat diet-induced NAFLD mice model

Kim,

Jang,

Baek

et al. 2023

Phys Act Nutr

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[Purpose] Exercise helps modify the lipid profile in the body, partly through its impact on sterol regulatory element binding protein-1 (SREBP-1) and peroxisome proliferator-activated receptor-γ (PPAR-γ). Individual differences in response to exercise and genetic variations may influence the response to PA. Therefore, this study explored Rosae multiflorae fructus (RMF) as a supplement candidate that improves exercise capacity and controls non-alcoholic fatty liver disease (NAFLD) by suppressing lipogenesis and controlling lipid peroxidation.[Methods] RMF is a natural herbal medicine used in Dongui Bogam. RMF has antioxidant, anti-inflammatory, and anti-allergic effects. However, the effects of RMF on NAFLD have not yet been investigated. In this study, we examined the effects of RMF in a mouse model of high-fat diet-induced NAFLD. Mouse livers were isolated and analyzed using H&E staining and immunohistochemistry.[Results] RMF downregulated lipid peroxidation markers, such as CYP2E1, in the livers of mice with high-fat diet-induced NAFLD. Additionally, the RMF significantly reduced the lipid accumulation-related protein expression of CD36, SREBP-1, and PPAR-γ.[Conclusion] RMF exerts anti-lipid peroxidation and anti-lipogenic effects in a high-fat diet-induced NAFLD mouse model.

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“…Osteoclast differentiation culture was performed with mouse BMMs as previously described. [ 15 , 16 ] In brief, bone marrow cells were flushed from the tibiae and femurs of 5-week-old female ICR mice and cultured in α-minimal essential medium (α-MEM) containing 10% fetal bovine serum (FBS) overnight on culture dishes. Adherent cells were discarded, and nonadherent cells were further incubated with 30 ng/mL M-CSF in Petri dishes for 3 days.…”

Section: Methodsmentioning

confidence: 99%

Effects of the Lysine Methyltransferase Inhibitor AZ505 on Bone Metabolism

et al. 2021

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Background: Protein methylation has important role in regulating diverse cellular responses, including differentiation, by affecting protein activity, stability, and interactions. AZ505 is an inhibitor of the SET and MYND domain-containing protein 2 lysine methylase. In this study, we investigated the effect of AZ505 on osteoblast and osteoclast differentiation in vitro and evaluated the effect of AZ505 in vivo on the long bones in mice.Methods: Osteoblast differentiation was assessed by alkaline phosphatase (ALP) and Alizarin red staining after culturing calvarial preosteoblasts in an osteogenic medium. Osteoclast differentiation was analyzed by tartrate-resistant acid phosphatase (TRAP) staining in bone marrow-derived macrophages cultured with macrophage-colony stimulating factor and receptor activator of nuclear factor-κB ligand (RANKL). For in vivo experiments, mice were intraperitoneally injected with AZ505 and femurs were examined by micro-computed tomography.Results: AZ505 increased ALP and Alizarin red staining in cultured osteoblasts and the expression of osteoblast marker genes, including Runx2 and osteocalcin. AZ505 resulted in decreased TRAP-staining of osteoclasts and expression of c-Fos and nuclear factor of activated T cells transcription factors and osteoclast marker genes, including cathepsin K and dendritic cell-specific transmembrane protein. Unexpectedly, in vivo administration of AZ505 markedly decreased the trabecular bone mass of femurs. In support of this catabolic result, AZ505 strongly upregulated RANKL expression in osteoblasts.Conclusions: The results indicate that AZ505 has a catabolic effect on bone metabolism in vivo despite its anabolic effect in bone cell cultures. The findings indicate that cell culture data should be extrapolated cautiously to in vivo outcomes for studying bone metabolism.

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Inhibitory Effect of Rosae Multiflorae Fructus Extracts on the Receptor Activator of NF-κB Ligand-Induced Osteoclastogenesis through Modulation of P38- and Ca²⁺-Mediated Nuclear Factor of Activated T-Cells Cytoplasmic 1 Expression

Cited by 4 publications

References 32 publications

Rosehip Extract-Functionalized Magnesium Hydroxide Nanoparticles and Its Effect on Osteoblastic and Osteoclastic Cells

Rosehip Extract-Functionalized Magnesium Hydroxide Nanoparticles and Its Effect on Osteoblastic and Osteoclastic Cells

Rosae multiflorae fructus regulates the lipogenesis in high-fat diet-induced NAFLD mice model

Effects of the Lysine Methyltransferase Inhibitor AZ505 on Bone Metabolism

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