Inhibitory effect of Capsicum chinense and Piper nigrum fruits, capsaicin and piperine on aflatoxins production in Aspergillus parasiticus by downregulating the expression of aflD, aflM, aflR, and aflS genes of aflatoxins biosynthetic pathway

Buitimea-Cantúa, Génesis V.; Buitimea‐Cantúa, Nydia E.; Rocha-Pizaña, María del Refugio; Morales, Alejandro; Magaña-Barajas, Elisa; Molina‐Torres, Jorge

doi:10.1080/03601234.2020.1787758

Cited by 18 publications

(8 citation statements)

References 32 publications

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“…Besides a strain’s toxigenicity, the substrate composition (NaCl, capsaicinoids, capsinoids, etc.) and ecophysiological factors (lower a w level, temperature) can play a key role in the up/downregulation of mycotoxin biosynthesis [ 54 , 55 ]. Concerning Merkén, despite being a pepper-based product, the high NaCl concentration (10% w / w ) makes this substrate an unlikely ecological habitat for the berry fruits of C. annuum L. cv.…”

Section: Resultsmentioning

confidence: 99%

“…Ochratoxin A has been previously detected in food substrates rich in salt such as cheese, dry-cured sausages, and, according to the results from this survey, in Merkén. In addition, the antifungal compounds (e.g., capsaicin) present in pepper powder and also Capsicum berry fruits can inhibit fungal growth including aflatoxigenic strains by downregulating AF biosynthesis [ 54 ]. The role of NaCl and capsaicinoids on fungal community dynamics and mycotoxin biosynthesis in C. annuum berry fruits and Merkén is still unclear.…”

Section: Resultsmentioning

confidence: 99%

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Occurrence of Aflatoxins and Ochratoxin A during Merkén Pepper Powder Production in Chile

Costa

Santos

Soares

et al. 2022

Foods

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Berry fruits of Capsicum annuum L. cv. “Cacho de Cabra” are used for the manufacture of a traditional pepper powder known as Merkén. In the present study, aflatoxins (AFs) and ochratoxin A (OTA) contamination in berry fruits of C. annuum was determined at harvest, drying, and smoking stages of Merkén production, in cumin and coriander seeds used as Merkén ingredients, and in the final packaged Merkén produced by local farmers. Additionally, Merkén samples from local markets in the region of La Araucanía (Chile) were also evaluated. Chromatographic analysis was based on a qualitative method. AFs and OTA were not detected on pepper pods and seeds. There was no detection of AFs and OTA on cultured Aspergillus and Penicillium strains isolated from pepper pods, cumin and coriander seeds and Merkén. The lack of AFs/OTA-producers among the isolated fungal species can explain and support the absence of contamination in pepper pods. In contrast, the AFB1 was detected in 75% of Merkén obtained from farmers and 46% of Merkén samples purchased from local markets; while OTA was detected in 100% of Merkén samples obtained from farmers and local markets. In the Merkén production chain, the harvest and post-harvest are key stages for fungal growth while the commercialization stage is highly susceptible to AFs and OTA contamination.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Occurrence of Aflatoxins and Ochratoxin A during Merkén Pepper Powder Production in Chile

Costa

Santos

Soares

et al. 2022

Foods

View full text Add to dashboard Cite

show abstract

“…flavus is lacking, and more importantly the function of lysine 2‐hydroxyisobutyrylation of this key enzyme in aflatoxin biosynthesis is still unclear and needs to be elucidated. Additionally, recent studies also indicate that AflM has specific catalytic activity and may serve as a promising biocontrol target in agriculture (Conradt et al ., 2015; Buitimea‐Cantúa et al ., 2020; Raruang et al ., 2020). Based on the above results, AflM was selected for further verification.…”

Section: Discussionmentioning

confidence: 99%

“…K hib at K49, K179 and K180 of AflM negatively regulates aflatoxin biosynthesis. Buitimea-Cantúa et al, 2020;Raruang et al, 2020). Based on the above results, AflM was selected for further verification.…”

Section: Discussionmentioning

confidence: 99%

Lysine 2‐hydroxyisobutyrylation orchestrates cell development and aflatoxin biosynthesis in Aspergillus flavus

Wang

Yang

et al. 2022

Environmental Microbiology

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Summary Lysine 2‐hydroxyisobutyrylation (Khib) is a recently identified post‐translational modification (PTM) that regulates numerous cellular metabolic processes. In pathogenic microorganism, although glycolysis and fungal virulence are regulated by Khib, its potential roles in fungi remain to be elusive. Our preliminary results showed that levels of Khib fluctuate over time in Aspergillus flavus, which frequently contaminates crops and produces carcinogenic aflatoxins. However, the perception of Khib function in A. flavus is limited, especially in mycotoxin‐producing strains. Here, we performed a comprehensive analysis of Khib in A. flavus, and 7156 Khib sites were identified in 1473 proteins. Notably, we demonstrated that Khib of AflM, a key enzyme in aflatoxin biosynthesis, affected conidia production and sclerotia formation. Furthermore, aflM deletion impaired aflatoxin biosynthesis, and more importantly, strains in which Khib was mimicked by K to T mutation at K49, K179 and K180 sites showed reduced aflatoxin production compared with wild type and ΔaflM complementation strains. These results indicate that Khib at these sites of AflM negatively regulates aflatoxin biosynthesis in A. flavus. In summary, our study revealed the potential roles of Khib in A. flavus, and particularly shed light on a new way to regulate aflatoxin production via Khib.

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“…Disruption or deletion of the a D (nor-1) gene leads to the accumulation of norsolorinic acid and blocks the synthesis of all a atoxins and their intermediates beyond norsolorinic acid. Ren et al (2020) and Buitimea-Cantua et al (2020) suggest inhibits AFs biosynthesis by A. avus via enhancing fungal oxidative stress response. Additionally, inactivating some enzymes in biosynthesis of AFs, in addition to confusion in the expression of a D, a M, a R, and a S genes of AFs biosynthetic pathway.…”

Section: Evaluation Of Aecp On Growth Toxigenic Aspergillus Fungimentioning

confidence: 99%

Protect peanut kernels from Aspergillus spp and their mycotoxins during storage by aqueous extract of carob pulp

El-Desouky

2022

Preprint

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Recently, the trend of many studies to replace synthetic antifungals commonly used during storage with natural antifungals; Plants extracts are one of the promising strategies for this purpose. Therefore, this study aimed to evaluate the aqueous extract of carob pulp (AECP) at 1.5, 2.5, and 5mg/ml on the growth of some toxigenic Aspergillus species, and its effectiveness as antimycotoxigenic (aflatoxins and ochratoxin A) during storage red peanut kernels (RPKs) for 90 days. AECP at 5 mg/ml affected inhibition growth, as Aspergillus ochraceus was the most affected, then A. aflatoxiformans recorded (15.7 ± 0.4) and (13.4 ± 0.5), respectively. Whereas study influence of AECP on the accumulation of total AFs and OTA produced by A. parasiticus and A. ochraceus during the storage of RPKs for 90 days. The results reflected that the active compounds in the AECP can protect RPKs for 45 days without detecting any amounts of total AFs and OTA at 5mg/ml. While after 90 days, the percentages of inhibition for the four types (AFB1, AFG1, AFB2, AFG2) and OTA after storage for 90 days compared with positive control samples (85.1, 76.5, 86.5,84.2, and 87.5%), respectively. Finally, AFB1 recorded the lowest inhibition percentages with all treatments; on the contrary, it was OTA.

show abstract

Inhibitory effect of Capsicum chinense and Piper nigrum fruits, capsaicin and piperine on aflatoxins production in Aspergillus parasiticus by downregulating the expression of aflD, aflM, aflR, and aflS genes of aflatoxins biosynthetic pathway

Cited by 18 publications

References 32 publications

Occurrence of Aflatoxins and Ochratoxin A during Merkén Pepper Powder Production in Chile

Occurrence of Aflatoxins and Ochratoxin A during Merkén Pepper Powder Production in Chile

Lysine 2‐hydroxyisobutyrylation orchestrates cell development and aflatoxin biosynthesis in Aspergillus flavus

Protect peanut kernels from Aspergillus spp and their mycotoxins during storage by aqueous extract of carob pulp

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