Inhibitory effect of Ephedrae herba, an oriental traditional medicine, on the growth of influenza A/PR/8 virus in MDCK cells

Mantani, Naoki; Andoh, Tsugunobu; Kawamata, Hiroshi; Terasawa, Katsutoshi; Ochiai, Hiroshi

doi:10.1016/s0166-3542(99)00067-4

Cited by 80 publications

(69 citation statements)

References 14 publications

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“…The exposure of the enveloped viruses such as HSV-1, HSV-2 and dengue viruses to the antiviral components prior to cell infection resulted in prevention of cell infection [3]. On the other hand, in a few studies, the plant antiviral ingredients showed to be effective against the intracellular steps of enveloped viruses such as HSV-1, bovine herpesvirus type 2, human immunodeficiency virus type 1, influenza A virus, influenza B virus, and human respiratory syncytial virus [11,12,16,19].…”

Section: Discussionmentioning

confidence: 99%

Antiviral activity of the oseltamivir and Melissa officinalis L. essential oil against avian influenza A virus (H9N2)

et al. 2016

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Lemon balm derivatives are going to acquire a novelty as natural and potent remedy for treatment of viral infections since the influenza viruses are developing resistance to the current antivirals widely. Oseltamivir, Melissa officinalis essential oil (MOEO) and their synergistic efficacy against avian influenza virus (AIV) subtype H9N2 were evaluated in vitro in MDCK cells at different time exposure by using TCID50, HA, Real Time PCR and HI assay. The results showed that MOEO could inhibit replication of AVI through the different virus replication phase (P B 0.05). Also the highest antiviral activity of MOEO was seen when AIV incubated with MOEO before cell infection. The TCID50/ml was reduced 1.3-2.1, 2.3-2.8, 3.7-4.5 log 10 than control group (5.6 log 10), HAU/50 ll was decreased 85-94, 71.4-94, 71.4-94 % and viral genome copy number/ll was brought down 68-95, 90-100, 89.6-99.9 % at pre-infection, post-infection and simultaneous stage, respectively. Hemagglutination inhibition result showed the MOEO was not able to inhibit agglutination of the chicken red blood cell (cRBC). Replication of the AVI was suppressed by the different concentration of oseltamivir completely or near 100 %. Also oseltamivir showed a synergistic activity with MOEO especially when oseltamivir concentration reduced under 0.005 mg/ml. The chemical composition was examined by GC-MS analysis and Its main constituents were identified as monoterpenaldehydes citral a, citral b. In conclusion, the findings of the study showed that lemon balm essential oil could inhibit influenza virus replication through different replication cycle steps especially throughout the direct interaction with the virus particles.

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Section: Discussionmentioning

confidence: 99%

Antiviral activity of the oseltamivir and Melissa officinalis L. essential oil against avian influenza A virus (H9N2)

et al. 2016

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“…Tanabe et al reported that genistein, the principal ingredient of JP Pueraria Root (Kakkon) in Kakkonto, suppresses the leucocyte-derived neutrophil chemotactic factor-1 and -2 in an air pouch-type allergic inflammation model in rats (11). Ephedra Herb (Mao) is known to have an inhibitory effect against the growth of influenza A/PR/8/34 (H1N1) virus in Madin-Darby canine kidney cells (12). Glycyrrhiza species, an active component of Kanzo, has also been shown to exhibit antiviral effects in several animal studies.…”

Section: Discussionmentioning

confidence: 99%

Non-superiority of Kakkonto, a Japanese Herbal Medicine, to a Representative Multiple Cold Medicine with Respect to Anti-aggravation Effects on the Common Cold: A Randomized Controlled Trial

et al. 2014

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Objective Kakkonto, a Japanese herbal medicine, is frequently used to treat the common cold not only with a physician's prescription, but also in self-medication situations. This study aimed to examine whether Kakkonto prevents the aggravation of cold symptoms if taken at an early stage of illness compared with a well-selected Western-style multiple cold medicine. Methods This study was a multicenter, active drug-controlled, randomized trial. Adults 18 to 65 years of age who felt a touch of cold symptoms and visited 15 outpatient healthcare facilities within 48 hours of symptoms onset were enrolled. The participants were randomly assigned to two groups: one treated with Kakkonto (Kakkonto Extract-A, 6 g/day) (n=209) and one treated with a Western-style multiple cold medicine (Pabron Gold-A, 3.6 g/day) (n=198) for at most four days. The primary outcome of this study was the aggravation of cold, nasal, throat or bronchial symptoms, scored as moderate or severe and lasting for at least two days within five days after entry into the study. Results Among the 410 enrollees, 340 (168 in the Kakkonto group and 172 in the Pabron group) were included in the analyses. The proportion of participants whose colds were aggravated was 22.6% in the Kakkonto group and 25.0% in the Pabron group (p=0.66). The overall severity of the cold symptoms was not significantly different between the groups. No harmful adverse events occurred in either group. Conclusion Kakkonto did not significantly prevent the progression of cold symptoms, even when prescribed at an early stage of the disease.

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“…Moreover, the results of in vitro studies on the components of Maoto have suggested that Maoto has direct antiviral effects. It has been reported that Ephedrae herba inhibits the growth of influenza viruses upon direct addition to cultures of infected cells owing to the suppression of acidified cellular compartments essential for the uncoating process of the influenza virus [21]. It has also been reported that trans-cinnamaldehyde, one of the principal constituents of essential oil derived from Cinnamomi cortex, induces a complete or partial reduction in influenza virus growth by the inhibition of viral protein synthesis [22].…”

Section: Discussionmentioning

confidence: 99%

Utility of Maoto in an Influenza Season Where Reduced Effectiveness of Oseltamivir Was Observed a Clinical, Non-Randomized Study in Children

Toriumi

Kamei²,

Murata³

et al. 2012

Forsch Komplementmed

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Background: In an influenza season where reduced effectiveness of oseltamivir was observed, we investigated the effectiveness of Maoto for influenza infection in children. Methods: Patients diagnosed with influenza by rapid diagnostic kit underwent treatment in one of the following groups: Maoto-treated group (group 1 (M)); oseltamivir-treated group (group 2 (O)); Maoto+oseltamivir-treated group (group 3 (M+O)); zanamivir-treated group (group 4 (Z)); and Maoto+zanamivir-treated group (group 5 (M+Z)). Results: In influenza A patients who completed the study (n = 150), the mean duration of fever after administration (DFA) was significantly shorter in group 3 (M+O) (31.1 h, p < 0.01) and in group 4 (Z) (35.2 h, p < 0.05), as compared to group 2 (O) (56.0 h). Among these, in patients aged ≤5 years (n = 54), DFA was significantly shorter in group 1 (M) (33.2 h, p < 0.05) and in group 3 (M+O) (34.6 h, p < 0.05), as compared to group 2 (O) (61.4 h). In influenza B patients who completed the study (n = 70), no significant differences in DFA were observed among the groups. Conclusion: Maoto may be useful, particularly in cases of influenza with low sensitivity to oseltamivir and in patients aged ≤5 years for whom the use of zanamivir is difficult.

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Inhibitory effect of Ephedrae herba, an oriental traditional medicine, on the growth of influenza A/PR/8 virus in MDCK cells

Cited by 80 publications

References 14 publications

Antiviral activity of the oseltamivir and Melissa officinalis L. essential oil against avian influenza A virus (H9N2)

Antiviral activity of the oseltamivir and Melissa officinalis L. essential oil against avian influenza A virus (H9N2)

Non-superiority of Kakkonto, a Japanese Herbal Medicine, to a Representative Multiple Cold Medicine with Respect to Anti-aggravation Effects on the Common Cold: A Randomized Controlled Trial

Utility of Maoto in an Influenza Season Where Reduced Effectiveness of Oseltamivir Was Observed a Clinical, Non-Randomized Study in Children

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Inhibitory effect of Ephedrae herba, an oriental traditional medicine, on the growth of influenza A/PR/8 virus in MDCK cells

Cited by 80 publications

References 14 publications

Antiviral activity of the oseltamivir and Melissa officinalis L. essential oil against avian influenza A virus (H9N2)

Antiviral activity of the oseltamivir and Melissa officinalis L. essential oil against avian influenza A virus (H9N2)

Non-superiority of Kakkonto, a Japanese Herbal Medicine, to a Representative Multiple Cold Medicine with Respect to Anti-aggravation Effects on the Common Cold: A Randomized Controlled Trial

Utility of Maoto in an Influenza Season Where Reduced Effectiveness of Oseltamivir Was Observed  a Clinical, Non-Randomized Study in Children

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Utility of Maoto in an Influenza Season Where Reduced Effectiveness of Oseltamivir Was Observed a Clinical, Non-Randomized Study in Children