Abstract:BackgroundCurcumin (diferuloylmethane) has been associated with the inhibition of angiogenesis, as well as the prevention of cancers and inflammatory processes. The aim of this study was to assess the efficacy of curcumin in suppressing angiogenesis in the cultured endothelial cells of rat aortic rings.MethodsEight-week-old male Wistar rats were randomized into five groups each with a different treatment and cell culturing paradigm: controls cultured in the absence of VEGF (vascular endothelial growth factor) … Show more
“… 14 Stimulation of angiogenesis is an important aspect for normal wound healing but hyperglycemic condition in diabetic wound disrupts angiogenesis and hypoxia inducible factor (HIF) -α trans-activation 15 , 16 . The vascular endothelial growth factor (VEGF) plays an important role in signaling and balancing of neo-vascularization 17 , 18 . The VEGF signaling cascade initiated by binding with transmembrane tyrosine kinase receptors – VEGFR-I and VEGFR-II.…”
Diabetic patients are frequently afflicted with impaired wound healing where linear progression of molecular and cellular events compromised. Despite of meaningful progress in diabetic treatment, management of diabetic chronic wounds is still challenging. Jamun (
Syzygium cumini
) honey may be a promising candidate for diabetic wound healing and need to explore in detail.
So present study was designed to evaluate the efficacy of Jamun honey (JH) for diabetic wound healing in
in vitro
wound (primary fibroblasts) model and in
in vivo
of diabetic mice (Streptozotocin induced) model. The fibroblast cell model was studied for migratory behaviour and myofibrolasts infiltration under honey interventions
via
scratch/migration assay, immuno-cytochemistry and western blot. We applied FDA approved Manuka honey (MH) as positive control and JH as test honey to evaluate wound re-epithelialization, sub-epithelial connective tissue modification and angiogenesis
via
histo-pathological and immuno-histochemical analysis. JH (0.1% v/v) dilution has notably improved wound closure, migration with concomitant α-SMA expressions
in vitro
. Topical application of JH in diabetic mice model showed significant (*p ≤ 0.05) wound closure, reepithelialization, collagen deposition (I/III) and balanced the myofibroblasts formation. It also modulated vital angiogenic markers (
viz
HIF-1α, VEGF, VEGF R–II) significantly (*p ≤ 0.05). All these observations depicted that JH promotes sequential stages of wound healing in diabetic mice model. The results of the present study established Jamun honey as good as Manuka honey considering wound closure, re-epithelialization, collagen deposition and pro-angiogenic potential.
“… 14 Stimulation of angiogenesis is an important aspect for normal wound healing but hyperglycemic condition in diabetic wound disrupts angiogenesis and hypoxia inducible factor (HIF) -α trans-activation 15 , 16 . The vascular endothelial growth factor (VEGF) plays an important role in signaling and balancing of neo-vascularization 17 , 18 . The VEGF signaling cascade initiated by binding with transmembrane tyrosine kinase receptors – VEGFR-I and VEGFR-II.…”
Diabetic patients are frequently afflicted with impaired wound healing where linear progression of molecular and cellular events compromised. Despite of meaningful progress in diabetic treatment, management of diabetic chronic wounds is still challenging. Jamun (
Syzygium cumini
) honey may be a promising candidate for diabetic wound healing and need to explore in detail.
So present study was designed to evaluate the efficacy of Jamun honey (JH) for diabetic wound healing in
in vitro
wound (primary fibroblasts) model and in
in vivo
of diabetic mice (Streptozotocin induced) model. The fibroblast cell model was studied for migratory behaviour and myofibrolasts infiltration under honey interventions
via
scratch/migration assay, immuno-cytochemistry and western blot. We applied FDA approved Manuka honey (MH) as positive control and JH as test honey to evaluate wound re-epithelialization, sub-epithelial connective tissue modification and angiogenesis
via
histo-pathological and immuno-histochemical analysis. JH (0.1% v/v) dilution has notably improved wound closure, migration with concomitant α-SMA expressions
in vitro
. Topical application of JH in diabetic mice model showed significant (*p ≤ 0.05) wound closure, reepithelialization, collagen deposition (I/III) and balanced the myofibroblasts formation. It also modulated vital angiogenic markers (
viz
HIF-1α, VEGF, VEGF R–II) significantly (*p ≤ 0.05). All these observations depicted that JH promotes sequential stages of wound healing in diabetic mice model. The results of the present study established Jamun honey as good as Manuka honey considering wound closure, re-epithelialization, collagen deposition and pro-angiogenic potential.
“…It has been also found that curcumin attenuates high glucose-induced neonatal rat cardiomyocyte apoptosis by inhibiting NADPH oxidasemediated oxidative stress and this protective effect is most likely mediated by PI3K/Akt-related signaling pathway [61]. A recent study showed that curcumin inhibits angiogenesis in a STZ-induced diabetic rat model with an aortic ring assay [62]. Curcumin also offers a protective effect against various organs and tissue damage that could occur either directly or indirectly as a result of the persistence of hyperglycemia.…”
Diabetes is a prevalent systemic disease affecting a significant proportion of the population worldwide. There is growing scientific evidence in connecting oxidative stress with the pathogenesis and development of diabetes and its secondary complications. Therefore, it seems reasonable that molecules with antioxidant activities can play an important role in the improvement of diabetes. In recent years, research showed that plant-derived polyphenols, due to their various biological properties, could be effective for the treatment of diabetes and its associated complications. In this review, we discuss the role of oxidative stress in diabetes and examine the impact of some plant-derived polyphenols with antioxidant properties, in relation to the development and progression of the disease. Several in vitro and animal studies showed that dietary plant polyphenols could modulate carbohydrate and lipid metabolism, attenuate hyperglycemia, dyslipidemia, and insulin resistance, improve adipose tissue metabolism, and alleviate oxidative stress and stress-sensitive signaling pathways and inflammatory processes. Polyphenolic compounds can also prevent the development of long-term diabetes complications including cardiovascular disease, neuropathy, nephropathy, and retinopathy. However, further investigations via human clinical studies are needed before these polyphenolic compounds can be used as therapeutic agents for reducing oxidative stress-associated diabetic complications.
“…Irregularities in the aortic segments have been identified as one of the drawbacks of this assay (Staton et al, ). To evaluate the effects of curcumin on angiogenesis, the aortic ring assay has been included in a study carried out by Dehghan et al (). Ben‐Mabrouk et al () have also used the aortic ring assay to report in vitro anti‐angiogenic effects of CC5 and CC8, disintegrins isolated from the venom of Cerastes cerastes viper.…”
Section: Assays and Techniques Used To Monitor Angiogenesismentioning
Development of a cancer is a multistep process and six major hallmarks of cancer that are known to control malignant transformation have been described. Anticancer drug development is a tedious process, requiring a number of in vitro, in vivo and clinical studies. In vitro assays provide an initial platform for cancer drug discovery approaches. A wide range of in vitro assays/techniques have been developed to evaluate each hallmark feature of cancer and selection of a particular in vitro assay or technique mainly depends on the specific research question (s) to be examined. In the present review, we have described some commonly utilized in vitro assays and techniques used to examine cell viability/proliferation, apoptosis, cellular senescence, invasion and migration, oxidative stress and antioxidant effects, gene and protein expression, angiogenesis and genomic alterations in cancer drug discovery. Additionally, uses of modern techniques such as high throughput screening, high content screening and reporter gene assays in cancer drug discovery have also been described.
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