Inhibitory Effect of Crocin Against Gastric Carcinoma via Regulating TPM4 Gene

Luo, Ying; Yu, Peng; Zhao, Jingbo; Guo, Qinghua; Fan, Baohua; Diao, Yinzhuo; Jin, Yulong; Wu, Jing; Zhang, Chengwu

doi:10.2147/ott.s254167

Cited by 15 publications

(12 citation statements)

References 31 publications

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“…The data showed the mRNA ( Figure 11A ) and protein expression ( Figure 11B ) levels of TPM4. In our previous study, after shRNA lentivirus infection, the knockdown efficiency of shTPM4 was 71.1%, and the knockdown of TPM4 inhibited GC cell proliferation ( 15 ) ( Supplementary Figure 2 ). In this research, wound healing and transwell assays were used to determine the migration potential of GC cells.…”

Section: Resultsmentioning

confidence: 90%

“…In our previous study, TPM4 was found to function as an oncogene that stimulates the proliferation of cells and prevents the death of cells by apoptosis both in vitro and in vivo . TPM4 was found to be expressed at higher levels in GC tissues than in paracancerous tissues ( 15 ). However, comprehensive studies on the relevance of TPM4 expression in tumor immune cell infiltration, ICP gene expression, TMB, MSI, NEO, ceRNA, drug sensitivity, and the pan-cancer mechanism of action of TPM4, especially in GC, have seldom been conducted.…”

Section: Introductionmentioning

confidence: 99%

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Integrated pan-cancer analysis and experimental verification of the roles of tropomyosin 4 in gastric cancer

Guo

Zhao

et al. 2023

Front. Immunol.

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ObjectiveTo investigate the function of tropomyosin 4 (TPM4) using pan-cancer data, especially in gastric cancer (GC), using comprehensive bioinformatics analysis and molecular experiments.MethodsWe used UCSC Xena, The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression Project (GTEx), TIMER2.0, GEPIA, cBioPortal, Xiantao tool, and UALCAN websites and databases for the extraction of pan-cancer data on TPM4. TPM4 expression was investigated with respect to prognosis, genetic alterations, epigenetic alterations, and immune infiltration. RNA22, miRWalk, miRDB, Starbase 2.0, and Cytoscape were used for identifying and constructing the regulatory networks of lncRNAs, miRNAs, and TPM4 in GC. Data from GSCALite, drug bank databases, and Connectivity Map (CMap) were used to analyze the sensitivity of drugs dependent on TPM4 expression. Gene Ontology (GO), enrichment analyses of the Kyoto Encyclopedia of Genes and Genomes (KEGG), wound healing assays, and (Matrigel) transwell experiments were used to investigate the biological functions of TPM4 in GC.ResultThe findings of the comprehensive pan-cancer analysis revealed that TPM4 has a certain diagnostic and prognosis value in most cancers. Alterations in the expression of TPM4, including duplications and deep mutations, and epigenetic alterations revealed that TPM4 expression is related to the expression of DNA methylation inhibitors and RNA methylation regulators at high concentrations. Besides, TPM4 expression was found to correlate with immune cell infiltration, immune checkpoint (ICP) gene expression, the tumor mutational burden (TMB), and microsatellite instability (MSI). Neoantigens (NEO) were also found to influence its response to immunotherapy. A lncRNA-miRNA -TPM4 network was found to regulate GC development and progression. TPM4 expression was related to docetaxel,5-fluorouracil, and eight small molecular targeted drugs sensitivity. Gene function enrichment analyses revealed that genes that were co-expressed with TPM4 were enriched within the extracellular matrix (ECM)-related pathways. Wound-healing and (Matrigel) transwell assays revealed that TPM4 promotes cell migration and invasion. TPM4, as an oncogene, plays a biological role, perhaps via ECM remodeling in GC.ConclusionsTPM4 is a prospective marker for the diagnosis, treatment outcome, immunology, chemotherapy, and small molecular drugs targeted for pan-cancer treatment, including GC treatment. The lncRNA-miRNA-TPM4network regulates the mechanism underlying GC progression. TPM4 may facilitate the invasion and migration of GC cells, possibly through ECM remodeling.

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Section: Resultsmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Integrated pan-cancer analysis and experimental verification of the roles of tropomyosin 4 in gastric cancer

Guo

Zhao

et al. 2023

Front. Immunol.

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“…A latest research by Xia et al [23] , demonstrated that overexpression of SLC7A2 suppressed the invasion and metastasis of hepatocellular carcinoma by regulating myeloid-derived suppressors cell recruitment. However, increased ELMO2 [24] and TPM4 [25] were related to the proliferation and metastasis of different cancers respectively, suggesting that they might contributed little to the regulation of miR-451 on RF/6A cell proliferation. RIC8A, was involved in the regulation of cell adhesion and migration.…”

Section: Discussionmentioning

confidence: 97%

miRNA-451 regulates rhesus choroid-retinal endothelial cell function and proteome profile

Wu¹,

Chen²,

Zhang³

et al. 2022

Int J Ophthalmol

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AIM: To evaluate the effect of miRNA-451 on rhesus macaque choroid-retinal endothelial (RF/6A) cell function and proteome profile. METHODS: The RF/6A cells were transfected with miRNA-451 mimic and inhibitor. The role of miRNA-451 on proliferation ability was evaluated by CCK-8 assay. Furthermore, iTRAQ quantitative proteomic analysis was applied to comprehensively illuminate the change of cellular proteins and biological function between different groups. RESULTS: In miRNA-451 overexpression group, cell proliferation of RF/6A decreased both at 24h and 48h; while in miRNA-451 inhibition group, on the contrary, RF/6A cell proliferation was increased at 48h. Based on iTRAQ quantitative proteomic analysis, 23 differentially expressed proteins (DEPs) were detected in the comparison of miRNA-451 mimic and mimic control-transfected RF/6A cells, and 30 DEPs were identified in the comparison of RF/6A cells transfected with miRNA-451 inhibitor and inhibitor control. DEPs such as GORASP2, KRT1, SLC7A2, RIC8A, DDX42, CAP1, PCBP2 might be closely related to the inhibitory effect of miRNA-451 on RF/6A cell proliferation, while PCYT1A, MGAT1, TUBB, MCU, SIL1, BID, MSH6 might account for the positive effect of miRNA-451 inhibitor on RF/6A cell growth. PTPN1, as the only protein exhibiting an opposite trend between miRNA-451 mimic and inhibitor-transfected cells, was most likely accountable for the inhibition of miRNA-451 mimic on RF/6A cell growth, and the promotion of miRNA-451 inhibitor on RF/6A cell proliferation. CONCLUSION: miRNA-451 overexpression can suppress the growth of RF/6A cells while knockdown of miRNA-451 can promote RF/6A cell viability. Among all DEPs, increased PTPN1 is most likely to account for the negative regulation of miRNA-451 on RF/6A proliferation. miRNA-451 can be a protective factor for neovascular disease of fundus via regulating choroid retinal endothelial cell function.

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“…Proteins encoded by CFL1, S100A6, ACTB, VIM, PFN1, ANXA2, RAN , and TPM4 have been found to be involved in the transport of actin–cofilin complex from the cytoplasm to nucleus, cell cycle process and differentiation, cell structure integrity and intercellular signal transfer, cytoskeleton stabilization and cytoplasmic integrity, regulation of cell growth, control of DNA synthesis, and regulation of microtubule polymerization during mitosis. All of these factors are critical in cell proliferation and differentiation (Filipek & Leśniak, 2020; Gibbs et al, 2019; Guo et al, 2013; Huang et al, 2020; Luo et al, 2021; Song et al, 2020; Wachsmannova et al, 2019; Z. L. Yang et al, 2013; C. Zhang et al, 2020). At the same time, the KEGG signal route was shown to be engaged in the Rap1 signal pathway, the Hippo signal pathway, focal adhesion, and other molecular signal pathways, all of which are significant in the activity of ADSCs stem cells.…”

Section: Discussionmentioning

confidence: 99%

“…At the same time, pseudo-time may be used to examine cell subsets or key genes of important differentiation nodes in the process of cell differentiation or development. This approach is now widely used to recreate the developmental pathway and differentiation (Filipek & Leśniak, 2020;Gibbs et al, 2019;Guo et al, 2013;Huang et al, 2020;Luo et al, 2021;Song et al, 2020;Wachsmannova et al, 2019;Z. L. Yang et al, 2013;C.…”

Section: Gene Expression Features Of Stemness In Distinct Adscs Clustersmentioning

confidence: 99%

Investigate the stemness of adult adipose‐derived stromal cells based on single‐cell RNA‐sequencing

Liu

Zhang

Yuan

et al. 2022

Cell Biology International

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The cellular heterogeneity and genetic features of stemness of adipose-derived stromal cells (ADSCs) remain unclear. Using single-cell RNA sequencing (scRNA-seq), we investigated the genomic features of the stemness gene in ADSCs with genetic variability. We cultured the ADSCs isolated from the fat waste of a healthy adult volunteers undergoing cosmetic plastic surgery to the third generation, used the BD Rhapsody platform to perform scRNA-seq, then used Monocle2 to analyze the growth and development trajectory of ADSCs, Cellular Trajectory Reconstruction Analysis Using Gene Counts and Expression (CytoTRACE) to evaluate the stemness gene characteristics in ADSCs clusters, and Beam to analyze the expression change characteristics of the main stemness related genes of ADSCs. According to the scRNA-seq data of 5325 ADSCs, they could be classified into nine cell clusters. According to CytoTRACE analysis, Cluster 3 of ADSCs had the highest stemness, whereas Cluster 8 had the lowest stemness.Pseudotime analysis revealed that Cluster 3 of ADSCs was primarily dispersed in the middle part of the growth and development trajectory, whereas Cluster 8 was primarily distributed at the end. We summarized the stemness of Cluster 3 in ADSCs with high expression of TPM1 and CCND1 genes in the metaphase of growth and development is the strongest, whereas the stemness of Cluster 8 with high expression of FICD, CREBRF, SDF2L1, HERPUD1, and HYOU1 genes in the telophase of growth and development is the weakest, providing a theoretical basis for screening and improving the therapeutic effect of ADSCs in cell transplantation research.

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Inhibitory Effect of Crocin Against Gastric Carcinoma via Regulating TPM4 Gene

Cited by 15 publications

References 31 publications

Integrated pan-cancer analysis and experimental verification of the roles of tropomyosin 4 in gastric cancer

Integrated pan-cancer analysis and experimental verification of the roles of tropomyosin 4 in gastric cancer

miRNA-451 regulates rhesus choroid-retinal endothelial cell function and proteome profile

Investigate the stemness of adult adipose‐derived stromal cells based on single‐cell RNA‐sequencing

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