Inhibitory effect of creosote and its main components on production of verotoxin of enterohaemorrhagic
            <i>Escherichia coli</i>
            O157:H7

Sakagami, Yoshikazu; Ichise, R.; Kajimura, K.; Yokoyama, H.

doi:10.1046/j.1365-2672.1999.00496.x

Cited by 5 publications

(4 citation statements)

References 2 publications

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“…However, softwoods are usually treated with copper–chromium–arsenic (CCA) or creosote on farms. Sakagami et al. (1999) found that creosote and its main phenolic components inhibited E. coli O157 verotoxin production.…”

Section: Discussionmentioning

confidence: 98%

“…However, softwoods are usually treated with copper-chromium-arsenic (CCA) or creosote on farms. Sakagami et al (1999) found that creosote and its main phenolic components inhibited E. coli O157 verotoxin production. It must be noted that the use of both CCA and creosote are being phased out because of environmental and health concerns and little information exists on how new preservatives may affect E. coli O157 survival.…”

Section: Discussionmentioning

confidence: 98%

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Persistence of Escherichia coli O157 on farm surfaces under different environmental conditions

et al. 2005

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Aims:To compare the persistence of Escherichia coli O157 on a variety of common faecally contaminated farmyard material surfaces (wood and steel) under different moisture and temperature regimes. Methods and Results: Samples of field-conditioned farmyard materials (galvanized steel and wood) were cut into pieces and contaminated with fresh cattle faeces inoculated with nontoxigenic E. coli O157 (strain 3704). Thereafter, they were stored at four different environmental conditions; with temperature (5 and 20°C) and moisture (moist or dry) as variables. Transfer of the pathogen to hands from the surfaces was also evaluated. Escherichia coli O157 numbers declined over time on all surfaces albeit at different rates according to the sample material and environmental conditions. Persistence was greatest on moist wood samples under cooler temperatures with large population numbers remaining after 28 days. Desiccation of surfaces resulted in a more rapid decline in E. coli O157 populations under both temperature regimes. Substantial numbers of colonies may also potentially be transferred to human hands from the surfaces during brief contact. Conclusions: When environmental conditions are favourable, E. coli O157 may persist for considerable times on a range of surfaces. However, when exposed to higher temperatures and dehydration, survival is notably decreased. Overall, bacterial persistence was significantly greater on wood samples relative to steel. Significance and Impact of the Study: Escherichia coli O157 is a prevalent pathogen, common in ruminant faeces. Contact with contaminated faeces may lead to human infection, resulting in possible severe illness. Although our study used only one strain of bacteria, our findings indicates that E. coli O157 has the potential to persist for long periods of time on gates, stiles and other farmyard surfaces under a range of environmental conditions. These farmyard surfaces therefore pose a potential infection pathway particularly where there is a high risk of direct human contact (e.g. child petting zoos, open farms).

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 98%

Persistence of Escherichia coli O157 on farm surfaces under different environmental conditions

et al. 2005

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“…It has been shown that several antibiotics inhibit the growth of E. coli O157:H7 and its production of verotoxin. In addition, certain extracts derived from clove (Syzygium aromaticum), creosote (Larrea tridentata), Limonium californicum, Cupressus lustianica, Salvia urica, and Jusiaea peruviana effectively inhibit or reduce verotoxin production (29,30). This is the first study to report that H. brasiletto extract affects verotoxin production in E. coli O157:H7.…”

Section: Discussionmentioning

confidence: 90%

Extracts of Haematoxylon brasiletto Inhibit Growth, Verotoxin Production, and Adhesion of Enterohemorrhagic Escherichia coli O157:H7 to HeLa Cells

Heredia

Escobar

Rodríguez‐Padilla

et al. 2005

Journal of Food Protection

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The extracts of 33 plants were evaluated for their effects on growth of Escherichia coli O157:H7 (EHEC). The extract of Haematoxylon brasiletto was the only one that effectively inhibited bacterial growth. The effects of ethanolic extracts of this plant on growth, verotoxin production, and adhesion of E. coli O157:H7 to HeLa cells were determined. The MBC for growth was 4 mg/ml. No verotoxin formation was detected at 1, 2, or 3 mg/ml. Preexposing bacteria and HeLa cells to various concentrations of extracts affected the adhesion between non-EHEC and HeLa cells. Partial purification of the active fraction suggested that polyphenols might play a role in the antimicrobial activity exhibited by H. brasiletto extracts.

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“…In the previous paper, 7) we examined the inhibitory effect of the creosote, a mixture of phenolic compounds, on the production of verotoxin (VT1 and VT2) by EHEC, and found that it inhibited verotoxin production. Furthermore, we also reported the inhibitory effect of clove, a famous crude drug on verotoxin.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effect of Plant Extracts on Production of Verotoxin by Enterohemorrhagic Escherichia coli O157 : H7.

Sakagami

Murata

Nakanishi

et al. 2001

JOURNAL OF HEALTH SCIENCE

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The inhibitory effect of several plant extracts on the production of verotoxin by enterohemorrhagic Escherichia coli O157 : H7 (EHEC) was investigated. The extracts from four plant species, Limonium californicum (Boiss.) A. Heller, Cupressus lustianica Miller, Salvia urica Epling and Jusiaea peruviana L., were effective on the inhibition for verotoxin production (31.3-125 µg/ml). The inhibition against verotoxin production was observed at a concentration lower than the minimal inhibitory concentration (MIC) of each extract of test plants (1000 µg/ml), indicating that these plant extracts would preferentially prevent the production of verotoxin rather than bactericidal effect on EHEC. These findings suggest that the administration of any appropriate plant extract might prevent the production of verotoxin on EHEC in the human intestines.

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Inhibitory effect of creosote and its main components on production of verotoxin of enterohaemorrhagic Escherichia coli O157:H7

Cited by 5 publications

References 2 publications

Persistence of Escherichia coli O157 on farm surfaces under different environmental conditions

Persistence of Escherichia coli O157 on farm surfaces under different environmental conditions

Extracts of Haematoxylon brasiletto Inhibit Growth, Verotoxin Production, and Adhesion of Enterohemorrhagic Escherichia coli O157:H7 to HeLa Cells

Inhibitory Effect of Plant Extracts on Production of Verotoxin by Enterohemorrhagic Escherichia coli O157 : H7.

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