Inhibitory effect of cranberry polyphenol on biofilm formation and cysteine proteases of <i>Porphyromonas gingivalis</i>

Yamanaka, Akihiro; Kouchi, T.; Kasai, Kouichi; Kato, Tsuyoshi; Ishihara, Kazuyuki; Okuda, K

doi:10.1111/j.1600-0765.2007.00982.x

Cited by 66 publications

(54 citation statements)

References 34 publications

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“…This result is of interest because AC-PACs did not seem to have antibiotic properties, even at the highest concentration tested, which would limit the development of resistance to them and would not disturb the oral ecology. The inhibition of biofilm formation by an uncharacterized polyphenol extract prepared from cranberry has been previously reported for Streptococcus mutans and P. gingivalis (5,11,31,45).…”

Section: Discussionmentioning

confidence: 99%

Anti- Porphyromonas gingivalis and Anti-Inflammatory Activities of A-Type Cranberry Proanthocyanidins

Howell

Grenier

2010

Antimicrob Agents Chemother

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A-type cranberry proanthocyanidins (AC-PACs) have recently been reported to be beneficial for human health, especially urinary tract health. The effect of these proanthocyanidins on periodontitis, a destructive disease of tooth-supporting tissues, needs to be investigated. The purpose of this study was to investigate the effects of AC-PACs on various virulence determinants of Porphyromonas gingivalis as well as on the inflammatory response of oral epithelial cells stimulated by this periodontopathogen. We examined the effects of AC-PACs on P. gingivalis growth and biofilm formation, adherence to human oral epithelial cells and proteincoated surfaces, collagenase activity, and invasiveness. We also tested the ability of AC-PACs to modulate the P. gingivalis-induced inflammatory response by human oral epithelial cells. Our results showed that while AC-PACs neutralized all the virulence properties of P. gingivalis in a dose-dependent fashion, they did not interfere with growth. They also inhibited the secretion of interleukin-8 (IL-8) and chemokine (C-C motif) ligand 5 (CCL5) but did not affect the secretion of IL-6 by epithelial cells stimulated with P. gingivalis. This anti-inflammatory effect was associated with reduced activation of the nuclear factor-B (NF-B) p65 pathway. AC-PACs may be potentially valuable bioactive molecules for the development of new strategies to treat and prevent P. gingivalis-associated periodontal diseases.

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Section: Discussionmentioning

confidence: 99%

Anti- Porphyromonas gingivalis and Anti-Inflammatory Activities of A-Type Cranberry Proanthocyanidins

Howell

Grenier

2010

Antimicrob Agents Chemother

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“…Meanwhile, researches have explored many biological activities of plants extracts for treatment of various diseases (13,16,22). For inhibition of oral pathogens, many plant extracts have been reported to have the potential on this purpose, for example, ethyl acetate extract of Camellia sinensis leaves was reported to decrease the incidence of dental caries (23) whereas water extract of Vaccinium macrocarpon fruits showed an inhibitory effect against protease enzyme of Porphyromonas gingivalis (24). Moreover, the extract from Salvadora persica stem can inhibit many oral pathogenic bacteria, such as S. mutans, Lactobacillus acidophilus, Aggregatibacter actinomycetemcomitans, and P. gingivalis (25).…”

Section: Discussionmentioning

confidence: 99%

Potential of <i>Piper betle</i> extracts on inhibition of oral pathogens

Phumat

Khongkhunthian

Wanachantararak

et al. 2017

DD&T

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“…These biological activities have mainly been attributed to the polyphenol fraction. Polyphenols are naturally present in a number of foods and drinks, such as tea (12), coffee (5), wine and grape juice (3,33), beer and hops (32), cranberry juice (11,34,36,37), cocoa (23), apple juice (38), and many others (28). In a previous study, we have also shown that people who regularly consume foods and drinks containing polyphenols have lower levels of culturable bacteria in their saliva and dental plaque (27).…”

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confidence: 99%

Drinking Habits Are Associated with Changes in the Dental Plaque Microbial Community

et al. 2010

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Caries and gingivitis are the most prevalent oral infectious diseases of humans and are due to the accumulation of dental plaque (a microbial biofilm) on the tooth surface and at the gingival margin, respectively. Several in vitro and in vivo studies have shown that many natural components of foods and beverages inhibit the adhesion of and/or exert activity against oral bacteria. These biological activities have mainly been attributed to the polyphenol fraction. In order to explore the possibility that diet can alter the dental plaque community, in this study we evaluated the composition of the microbiota of supra-and subgingival plaque samples collected from 75 adult subjects with different drinking habits (drinkers of coffee, red wine, or water for at least 2 years) by analyzing the microbial population through the separation of PCR-amplified fragments using the denaturing gradient gel electrophoresis (DGGE) technique. The mean numbers of bands of the DGGE profiles from all three categories were evaluated. There were no significant differences between the two kinds of plaque collected from the control group (water drinkers), and this group showed the highest number of bands (supragingival plaque, 18.98 ؎ 3.16 bands; subgingival plaque, 18.7 ؎ 3.23 bands). The coffee and wine drinker groups generated the lowest numbers of bands for both supragingival plaque (coffee drinkers, 8.25 ؎ 3.53 bands; wine drinkers, 7.93 ؎ 2.55 bands) and subgingival plaque (coffee drinkers, 8.3 ؎ 3.03 bands; wine drinkers, 7.65 ؎ 1.68 bands). The differences between coffee drinkers or wine drinkers and the control group (water drinkers) were statistically significant. A total of 34 microorganisms were identified, and the frequency of their distribution in the three subject categories was analyzed. A greater percentage of subjects were positive for facultative aerobes when supragingival plaque was analyzed, while anaerobes were more frequent in subgingival plaque samples. It is noteworthy that the frequency of identification of anaerobes was significantly reduced when the frequencies for coffee and wine drinkers were compared with the frequencies for subjects in the control group. The DGGE profiles of the organisms in both plaque samples from all groups were generated and were used to construct dendrograms. A number of distinct clusters of organisms from water, coffee, and wine drinkers were formed. The clustering of some of the DGGE results into cohort-specific clusters implies similarities in the microbiotas within these groups and relevant differences in the microbiotas between cohorts. This supports the notion that the drinking habits of the subjects may influence the microbiota at both the supragingival and the subgingival levels.

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Inhibitory effect of cranberry polyphenol on biofilm formation and cysteine proteases of Porphyromonas gingivalis

Abstract: These findings indicate that the polyphenol fraction inhibits biofilm formation and the Arg-gingipain and Lys-gingipain activities of P. gingivalis.

Cited by 66 publications

References 34 publications

Anti- Porphyromonas gingivalis and Anti-Inflammatory Activities of A-Type Cranberry Proanthocyanidins

Anti- Porphyromonas gingivalis and Anti-Inflammatory Activities of A-Type Cranberry Proanthocyanidins

Potential of <i>Piper betle</i> extracts on inhibition of oral pathogens

Drinking Habits Are Associated with Changes in the Dental Plaque Microbial Community

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