Inhibitory Effect of Cranberry Polyphenol on Cariogenic Bacteria

Yamanaka-Okada, Ayumi; Sato, Emiko; Kouchi, T.; Kimizuka, Ryuta; Kato, Tsuyoshi; Okuda, K

doi:10.2209/tdcpublication.49.107

Cited by 45 publications

(36 citation statements)

References 16 publications

(4 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Patients with high dental caries scores demonstrated a higher prevalence of hydrophobic bacteria than low caries patients. 66 In addition, compounds that diminish the surface hydrophobicity of S. mutans are now being reported to inhibit bacterial adherence in vitro 67–69 and cariogenicity in an animal model. 67 …”

Section: Resultsmentioning

confidence: 99%

Binding Forces of Streptococcus mutans P1 Adhesin

Sullan

Crowley

et al. 2015

ACS Nano

View full text Add to dashboard Cite

Streptococcus mutans is a Gram-positive oral bacterium that is a primary etiological agent associated with human dental caries. In the oral cavity, S. mutans adheres to immobilized salivary agglutinin (SAG) contained within the salivary pellicle on the tooth surface. Binding to SAG is mediated by cell surface P1, a multifunctional adhesin that is also capable of interacting with extracellular matrix proteins. This may be of particular importance outside of the oral cavity as S. mutans has been associated with infective endocarditis and detected in atherosclerotic plaque. Despite the biomedical importance of P1, its binding mechanisms are not completely understood. In this work, we use atomic force microscopy-based single-molecule and single-cell force spectroscopy to quantify the nanoscale forces driving P1-mediated adhesion. Single-molecule experiments show that full-length P1, as well as fragments containing only the P1 globular head or C-terminal region, binds to SAG with relatively weak forces (~50 pN). In contrast, single-cell analyses reveal that adhesion of a single S. mutans cell to SAG is mediated by strong (~500 pN) and long-range (up to 6000 nm) forces. This is likely due to the binding of multiple P1 adhesins to self-associated gp340 glycoproteins. Such a cooperative, long-range character of the S. mutans–SAG interaction would therefore dramatically increase the strength and duration of cell adhesion. We also demonstrate, at single-molecule and single-cell levels, the interaction of P1 with fibronectin and collagen, as well as with hydrophobic, but not hydrophilic, substrates. The binding mechanism (strong forces, cooperativity, broad specificity) of P1 provides a molecular basis for its multifunctional adhesion properties. Our methodology represents a valuable approach to probe the binding forces of bacterial adhesins and offers a tractable methodology to assess anti-adhesion therapy.

show abstract

Section: Resultsmentioning

confidence: 99%

Binding Forces of Streptococcus mutans P1 Adhesin

Sullan

Crowley

et al. 2015

ACS Nano

View full text Add to dashboard Cite

show abstract

“…Earlier studies demonstrated a high-molecular-weight mass, nondialyzable material (NDM) prepared from cranberries to contain polyphenolic compounds that inhibited the secretion of proteolytic enzymes by periodontopathogens [9], adhesion of a number of bacterial species [10, 11], and were also shown to interfere with coaggregation of oral bacterial species and biofilm formation by Streptococcus mutans [3, 12]. The polyphenol fraction of cranberry was reported to decrease the hydrophobicity of streptococcal species [12, 13]. …”

Section: Introductionmentioning

confidence: 99%

Inhibition ofStreptococcus gordoniiMetabolic Activity in Biofilm by Cranberry Juice High-Molecular-Weight Component

Babu

Blair

Shiloah

et al. 2012

Journal of Biomedicine and Biotechnology

View full text Add to dashboard Cite

Previous studies demonstrated that a cranberry high-molecular-mass, nondialyzable material (NDM) can inhibit adhesion of numerous species of bacteria and prevents bacterial coaggregation of bacterial pairs. Bacterial coaggregation leads to plaque formation leading to biofilm development on surfaces of oral cavity. In the present study, we evaluated the effect of low concentrations of NDM onStreptococcus gordoniimetabolic activity and biofilm formation on restorative dental surfaces. We found that the NDM selectively inhibited metabolic activity ofS. gordonii, without affecting bacterial viability. Inhibiting the metabolic activity of bacteria in biofilm may benefit the health of the oral cavity.

show abstract

“…[1819] Weiss and colleagues in 2004 incorporated the NDM fraction of cranberry in a mouth rinse and subjects who used it for 6 weeks reported fewer cariogenic Streptococcus mutans . [20] In yet another study, cranberry fraction demonstrated the deactivation of enzymes glucosyltranferase and fructosyltranferase.…”

Section: Introductionmentioning

confidence: 99%

“…Besides, it also prevented the adhesion of P. gingivalis to various proteins including type I collagen thus reducing bacterial coaggregation in periodontal diseases. [1924]…”

Section: Introductionmentioning

confidence: 99%

Exploring the role of cranberry polyphenols in periodontits: A brief review

Mukherjee

Bandyopadhyay

Kundu

2014

J Indian Soc Periodontol

View full text Add to dashboard Cite

Cranberry juice polyphenols have gained importance over the past decade due to their promising health benefits. The bioactive component, proanthocyanidins is mainly responsible for its protective effect. A lot has been said about its role in urinary tract infection and other systemic diseases, but little is known about its oral benefits. An extensive search was carried out in the PubMed database using the terms “cranberry polyphenols” and “periodontitis” together. The institute library was also thoroughly scrutinized for all relevant information. Thus, a paper was formulated, the aim of which was to review the role of high molecular weight cranberry fraction on oral tissues and periodontal diseases.

show abstract

Inhibitory Effect of Cranberry Polyphenol on Cariogenic Bacteria

Cited by 45 publications

References 16 publications

Binding Forces of Streptococcus mutans P1 Adhesin

Binding Forces of Streptococcus mutans P1 Adhesin

Inhibition ofStreptococcus gordoniiMetabolic Activity in Biofilm by Cranberry Juice High-Molecular-Weight Component

Exploring the role of cranberry polyphenols in periodontits: A brief review

Contact Info

Product

Resources

About