Inhibitory effect of Coptidis Rhizoma and berberine on the proliferation of human esophageal cancer cell lines

Iizuka, Norio; Miyamoto, Koji; Okita, Kiwamu; Tangoku, Akira; Hayashi, Hiroto; Yosino, Shigehumi; Abe, Toshihiro; Morioka, T; Hazama, Shoichi; Oka, M.

doi:10.1016/s0304-3835(99)00264-5

Cited by 154 publications

(114 citation statements)

References 20 publications

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“…In a previous study, we identified Coptidis rhizoma as an agent possessing potent antitumor activity. 8,9 We also showed that C. rhizoma significantly attenuated weight loss in nude mice bearing IL-6 -producing esophageal cancer cells at a dose that does not alter tumor growth. 10 However, we were unable to clarify the relation between IL-6 and the anticachectic effect of C. rhizoma in this model.…”

mentioning

confidence: 78%

Anticachectic effects of the natural herb Coptidis rhizoma and berberine on mice bearing colon 26/clone 20 adenocarcinoma

Iizuka

Hazama

Yoshimura

et al. 2002

Intl Journal of Cancer

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We previously showed that the natural herb Coptidis rhizoma has an anticachectic effect in nude mice bearing human esophageal cancer cells. We further investigated this phenomenon by examining the anticachectic effect of C. rhizoma in syngeneic mice bearing colon 26/clone 20 carcinoma cells, which cause IL-6 -related cachexia after cell injection. We evaluated nutritional parameters such as serum glucose level and wasting of adipose tissue and muscle in tumor-bearing and non-tumor-bearing mice treated with C. rhizoma (CR) supplement or a normal diet. IL-6 levels in those mice were quantified by ELISA and real-time RT-PCR. CR supplementation significantly attenuated weight loss in tumor-bearing mice without changing food intake or tumor growth. Furthermore, these mice maintained good nutritional status. IL-6 mRNA levels in tumors and spleens and IL-6 protein levels in tumors and sera were significantly lower in tumorbearing mice treated with CR supplement than in those treated with a normal diet. CR supplementation did not affect food intake, body weight, nutritional parameters and IL-6 levels in non-tumor-bearing mice. An in vitro study showed that C. rhizoma and its major component, berberine, inhibited IL-1-induced IL-6 mRNA expression in a dose-dependent manner in colon 26/clone 20 cells. Our results showed that C. rhizoma exerts an anticachectic effect on colon 26/clone 20 -transplanted mice and that its effect is associated with tumor IL-6 production. We also suggest that its effect might be due to berberine.

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mentioning

confidence: 78%

Anticachectic effects of the natural herb Coptidis rhizoma and berberine on mice bearing colon 26/clone 20 adenocarcinoma

Iizuka

Hazama

Yoshimura

et al. 2002

Intl Journal of Cancer

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“…It has been employed in Chinese medicine for the treatment of diabetes mellitus. A great deal of evidence suggests that it may also prove effective against inflammation (Iizuka et al, 2000a), cancer cell proliferation (Iizuka et al, 2000b(Iizuka et al, , 2002, and the induction of novel growth-related genes, most notably rcl (Chan et al, 2002). We recently reported that the pretreatment of RIN cells with Coptidis rhizoma extract (CRE) prevented S-nitroso-N-acetylpenicillamine-induced apoptotic cell death (Kwon et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Coptidis rhizoma extract protects against cytokine-induced death of pancreatic β-cells through suppression of NF-κ B activation

Kim

Kwon²,

Han³

et al. 2007

Exp Mol Med

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We demonstrated previously that Coptidis rhizoma extract (CRE) prevented S-nitroso-N-acetylpenicillamine-induced apoptotic cell death via the inhibition of mitochondrial membrane potential disruption and cytochrome c release in RINm5F (RIN) rat insulinoma cells. In this study, the preventive effects of CRE against cytokine-induced β-cell death was assessed. Cytokines generated by immune cells infiltrating pancreatic islets are crucial mediators of β-cell destruction in insulin-dependent diabetes mellitus. The treatment of RIN cells with IL-1β and IFN-γ resulted in a reduction of cell viability. CRE completely protected IL-1β and IFN-γ-mediated cell death in a concentration-dependent manner. Incubation with CRE induced a significant suppression of IL-1β and IFN-γ-induced nitric oxide (NO) production, a finding which correlated well with reduced levels of the iNOS mRNA and protein. The molecular mechanism by which CRE inhibited iNOS gene expression appeared to involve the inhibition of NF-κB activation. The IL-1β and IFN-γ-stimulated RIN cells showed increases in NF-κB binding activity and p65 subunit levels in nucleus, and IκBα degradation in cytosol compared to unstimulated cells. Furthermore, the protective effects of CRE were verified via the observation of reduced NO generation and iNOS expression, and normal insulin-secretion responses to glucose in IL-1β and IFN-γ-treated islets.

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“…The potential effectiveness of berberine is indicated by its use in the Indian Ayurvedic (10), Unani, and Chinese systems of medicine since time immemorial. Berberine has been shown to possess antiinflammatory activities in vivo (11) and preliminary studies have been conducted to determine its anticarcinogenic activity in skin (12). It has been shown to inhibit activator protein 1, a key transcription factor in inflammation and carcinogenesis, in human cell lines (13) and has been shown to possess antitumor properties and effectively inhibit cyclooxygenase-2 transcriptional activity in human colon cancer cells (14,15).…”

Section: Introductionmentioning

confidence: 99%

Berberine, a natural product, induces G1-phase cell cycle arrest and caspase-3-dependent apoptosis in human prostate carcinoma cells

Mantena¹,

Sharma²,

Katiyar

2006

Molecular Cancer Therapeutics

314

216

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Berberine, a naturally occurring isoquinoline alkaloid, has been shown to possess anti-inflammatory and antitumor properties in some in vitro systems. Here, we report that in vitro treatment of androgen-insensitive (DU145 and PC-3) and androgen-sensitive (LNCaP) prostate cancer cells with berberine inhibited cell proliferation and induced cell death in a dose-dependent (10 -100 Mmol/L) and timedependent (24 -72 hours) manner. Treatment of nonneoplastic human prostate epithelial cells (PWR-1E) with berberine under identical conditions did not significantly affect their viability. The berberine-induced inhibition of proliferation of DU145, PC-3, and LNCaP cells was associated with G 1 -phase arrest, which in DU145 cells was associated with inhibition of expression of cyclins D1, D2, and E and cyclin-dependent kinase (Cdk) 2, Cdk4, and Cdk6 proteins, increased expression of the Cdk inhibitory proteins (Cip1/p21 and Kip1/p27), and enhanced binding of Cdk inhibitors to Cdk. Berberine also significantly (P < 0.05 -0.001) enhanced apoptosis of DU145 and LNCaP cells with induction of a higher ratio of Bax/Bcl-2 proteins, disruption of mitochondrial membrane potential, and activation of caspase-9, caspase-3, and poly(ADP-ribose) polymerase. Pretreatment with the pan-caspase inhibitor z-VAD-fmk partially, but significantly, blocked the berberineinduced apoptosis, as also confirmed by the comet assay analysis of DNA fragmentation, suggesting that berberineinduced apoptosis of human prostate cancer cells is mediated primarily through the caspase-dependent pathway. The effectiveness of berberine in checking the growth of androgen-insensitive, as well as androgensensitive, prostate cancer cells without affecting the growth of normal prostate epithelial cells indicates that it may be a promising candidate for prostate cancer therapy.

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Inhibitory effect of Coptidis Rhizoma and berberine on the proliferation of human esophageal cancer cell lines

Cited by 154 publications

References 20 publications

Anticachectic effects of the natural herb Coptidis rhizoma and berberine on mice bearing colon 26/clone 20 adenocarcinoma

Anticachectic effects of the natural herb Coptidis rhizoma and berberine on mice bearing colon 26/clone 20 adenocarcinoma

Coptidis rhizoma extract protects against cytokine-induced death of pancreatic β-cells through suppression of NF-κ B activation

Berberine, a natural product, induces G1-phase cell cycle arrest and caspase-3-dependent apoptosis in human prostate carcinoma cells

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