Inhibitory effect of conjugated dienoic derivatives of linoleic acid and β-carotene on the in vitro growth of human cancer cells

Shultz, Terry D.; Chew, Boon P.; Seaman, Wanda; Luedecke, L.O.

doi:10.1016/0304-3835(92)90062-z

Cited by 204 publications

(141 citation statements)

References 18 publications

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“…From the point of view of the experimental procedure, the levels of rumenic acid added to the medium in the present study (55 µM) were similar to those used in different systems such as human cancer cells [31] and isolated rat hepatocytes [32], and were lower than those used in monolayer cultures of bovine hepatocytes [33]. In all cases, no cytotoxic effects occurred as assessed by cell viability measurement [32].…”

Section: Discussionsupporting

confidence: 55%

In vitro metabolism of rumenic acid in bovine liver slices

et al. 2005

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-Ruminant products are the major source of CLA for humans. However, during periods of fat mobilization, the liver might play an important role in CLA metabolism which would limit the availability of the latter for muscles and milk. In this context, rumenic acid (cis-9, trans-11 CLA) metabolism in the bovine liver (n = 5) was compared to that of oleic acid (n = 3) by using the in vitro liver slice method. Liver slices were incubated for 17 h in a medium containing 0.75 mM of FA mixture and 55 µM of either [1-14 C] rumenic acid or [1-14 C] oleic acid at 37 °C under an atmosphere of 95% O 2 -5% CO 2 . Rumenic acid uptake by liver slices was twice (P = 0.009) that of oleic acid. Hepatic oxidation of both FA (> 50% of incorporated FA) led essentially to the production of acidsoluble products and to a lower extent to CO 2 production. Rumenic acid was partly converted (> 12% of incorporated rumenic acid) into conjugated C18:3. CLA and its conjugated derivatives were mainly esterified into polar lipids (71.7%), whereas oleic acid was preferentially esterified into neutral lipids (59.8%). Rumenic acid secretion as part of VLDL particles was very low and was onefourth lower than that of oleic acid. In conclusion, rumenic acid was highly metabolized by bovine hepatocytes, especially by the oxidation pathway and by its conversion into conjugated C18:3 for which the biological properties need to be elucidated. rumenic acid / oleic acid / metabolism / liver / bovine Abbreviations: ASP, acid-soluble products; BSA, bovine serum albumin; CLA, conjugated linoleic acid; FA, fatty acids; NL, neutral lipids; PL, polar lipids; VLDL, very-low density lipoproteins.

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Section: Discussionsupporting

confidence: 55%

In vitro metabolism of rumenic acid in bovine liver slices

et al. 2005

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“…Conjugated linoleic acid is also involved in the inhibition of mouse forestomach and rat mammary tumorigenesis (Ha et al 1990;Ip et al 1991). Shultz et al (1992) found that physiological concentrations of CLA are cytostatic and cytotoxic to human malignant melanoma, colorectal and breast cancer cells in vitro. More recently, CLA was reported to be effective in preventing the catabolic effects of immune stimulation (Cook et al 1993;Miller et al 1994).…”

Section: Introductionmentioning

confidence: 98%

Production of conjugated linoleic acid by dairy starter cultures

Jiang

Björck

Fondén

1998

Journal of Applied Microbiology

259

218

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J. JIANG, L. BJÖ RCK AND R. FONDÉ N. 1998. Nineteen different strains of lactobacilli, lactococci, streptococci and propionibacteria commonly used as dairy starter cultures were tested for their ability to produce conjugated linoleic acid (CLA) from free linoleic acid in vitro. Two strains of Propionibacterium freudenreichii ssp. freudenreichii and one strain of P. freudenreichii ssp. sheramnii were found to be capable of converting free linoleic acid to extracellular CLA. The highest level of CLA formed in the media was 265 mg ml −1 . Of the different isomers, cis-and trans-9,11-octadecadienoic acid represented more than 70% of the total CLA formed. The inhibitory effect of linoleic acid on the growth of the bacteria and its conversion to CLA in different media by propionibacteria are discussed.

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“…Moreover, CLA was also effective in reducing the size and metastasis of transplanted human breast cancer cells and prostate cancer cells in SCID mice [32,33] . Several studies [34][35][36][37][38][39][40][41][42][43] suggested that CLA was cytostatic and cytotoxic to a variety of human cancer cells in vitro, including hepatoma, malignant melanoma, colorectal cancer, breast carcinoma, and gastric cancer. One of our previous studies showed that c9,t11-CLA could inhibit the invasion of mouse melanoma cells (B16-MB) through reducing their adhesion ability to extracellular matrix [44] .…”

Section: Introductionmentioning

confidence: 99%

Effects ofc9,t11-conjugated linoleic acid on adhesion of human gastric carcinoma cell line SGC-7901

Chen

Yang²,

Wang³

et al. 2004

WJG

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AIM:To investigate the effect of c9,t11-conjugated linoleic acid (c9,t11-CLA) on the adhesion of human gastric carcinoma cell line (SGC-7901). METHODS:SGC-7901 cells were at first treated with different concentrations (25, 50, 100, 200 µmol/L) of c9, t11-CLA and 1 mL/L ethanol (as a negative control) for 24 h. Using adhesion assay and Western blot, we investigated the ability of SGC-7901 cells to adhere to intracellular matrix and examined the expression of E-cadherin (ECD), α-catenin, intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in these cells. RESULTS:

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Inhibitory effect of conjugated dienoic derivatives of linoleic acid and β-carotene on the in vitro growth of human cancer cells

Cited by 204 publications

References 18 publications

In vitro metabolism of rumenic acid in bovine liver slices

In vitro metabolism of rumenic acid in bovine liver slices

Production of conjugated linoleic acid by dairy starter cultures

Effects ofc9,t11-conjugated linoleic acid on adhesion of human gastric carcinoma cell line SGC-7901

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