Inhibitory effect of Asparagus cochinchinensis on tumor necrosis factor-alpha secretion from astrocytes

Kim, Hyung-Min; Lee, Eun-Hee; Lim, T. K.; Jung, Jin-Ah; Lyu, Yeoung-Su

doi:10.1016/s0192-0561(98)00022-8

Cited by 43 publications

(27 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Astrocytes were prepared from brains of newborn rats as described elsewhere (13). After 3 days in subculture, more than 95% of these first-passage cells were positive for glial fibrillary acidic protein (GFAP) as indicated in immunofluorescent studies that made use of anti-GFAP antiserum.…”

Section: Astrocyte Culturesmentioning

confidence: 99%

INHIBITORY EFFECT OF TUMOR NECROSIS FACTOR-α SECRETION FROM RAT ASTROCYTES BY CHILBOKEUM

Kim

Shin

2001

Immunopharmacology and Immunotoxicology

View full text Add to dashboard Cite

Substance P (SP) can stimulate secretion of tumor necrosis factor-alpha (TNF-alpha) from astrocytes stimulated with lipopolysaccharide (LPS). In this study, we have examined whether an aqueous extract of Chilbokeum inhibits secretion of TNF-alpha from primary cultures of rat astrocytes. Chilbokeum (10 microg/ml) significantly inhibited the TNF-alpha secretion by astrocytes stimulated with LPS and SP. Interleukin-1 (IL-1) has been shown to elevate TNF-alpha secretion from LPS-stimulated astrocytes while having no effect on astrocytes in the absence of LPS. We therefore examined whether IL-1 mediated inhibition of TNF-alpha secretion from primary astrocytes by Chilbokeum. Treatment of Chilbokeum (10 and 100 microg/ml) to astrocytes stimulated with both LPS and SP decreased IL-1 secretion significantly. Moreover, the secretion of TNF-alpha by LPS and SP in astrocytes was progressively inhibited with increasing amount of IL-1 neutralizing antibody. Our results suggest that Chilbokeum may inhibit TNF-alpha secretion by inhibiting IL-1 secretion and that Chilbokeum has an antiinflammatory activity in the central nervous system.

show abstract

Section: Astrocyte Culturesmentioning

confidence: 99%

INHIBITORY EFFECT OF TUMOR NECROSIS FACTOR-α SECRETION FROM RAT ASTROCYTES BY CHILBOKEUM

Kim

Shin

2001

Immunopharmacology and Immunotoxicology

View full text Add to dashboard Cite

show abstract

“…험모델에서 강한 radical scavenging 능력으로 항산화효과를 가지며 [15], TNF-α 분비를 억제시켜 중추신경계에서 항염증 효과가 있다고 발표되었다 [13,14]. 최근에는 천문동의 메탄올 추출물이 세포와 동물 실험을 통해 신경보호효과가 있다고 알려져 있다 [12].…”

Section: 천문동(Asparagus Cochinchinensis)은 중국 일본 한국 등에 서 자라는 다육생의 식물로unclassified

Ethanol Extract from Asparagus Cochinchinensis Attenuates Glutamate-Induced Oxidative Toxicity in HT22 Hippocampal Cells

Pak¹,

Choi²

2016

Journal of Life Science

View full text Add to dashboard Cite

We investigated the neuroprotective effect of an ethanol extract from Asparagus cochinchinensis (AC) against glutamate-induced toxicity in the HT22 hippocampal cell, which is an ideal in vitro model for oxidative stress. The neuroprotective effects of AC in HT22 cells were evaluated by analyzing cell viability, lactate dehydrogenase (LDH), flow cytometry for cell death types, reactive oxygen species (ROS), mitochondria membrane potential (MMP), and Western blot assays. In the cell death analysis, AC treatment resulted in significantly attenuated glutamate-induced loss of cell viability with a decrease in LDH release. AC treatment also reduced glutamate-induced apoptotic cell death. In the ROS and MMP analysis, AC treatment inhibited the elevation of intracellular ROS induced by glutamate exposure and the disruption of MMP. In oxidative stress-related proteins analysis, AC treatment inhibited the expression of poly ADP ribose polymerase and heme oxygenase-1 by glutamate. These results indicate that AC exerts a significant neuroprotective effect against glutamate-induced hippocampal damage by decreasing ROS production and stabilizing MMP. Thus, AC potentially provides a new strategy for the treatment of oxidative stress-related diseases.

show abstract

“…Dispersed cells were then plated in 24-well tissue culture plates at a concentration of 2 Â 10 5 cells/well. The adherent cells were identified as murine microglial cells and astrocytes using immunofluorescent staining with monoclonal anti-Mac-1 antibody (Hassan et al, 1991) and anti-GFAP antibody (Kim et al, 1998a). These cultures were >99% Mac-1 positive and GFAP negative as determined by immunohistochemistry indicating that they were composed of microglial cells.…”

Section: Isolation Of Murine Microglial Cellsmentioning

confidence: 99%

SunghyangJungki‐San Ga Pogongyoung inhibits IL‐1 mediated tumour necrosis factor‐α secretion in astrocytes

Kim

Hong

Lee

et al. 2002

Phytotherapy Research

View full text Add to dashboard Cite

Astrocytes play an important role in initiating and modulating inflammatory responses within the central nervous system (CNS). Extensive studies in rodents have shown that substance P induces inflammatory cytokine production in astrocytes. In this study we have examined whether an aqueous extract of SunghyangJungki-San Ga Pogongyoung (SSGP) inhibits the secretion of TNF-alpha from primary cultures of rat astrocytes. SSGP (10-1,000 microg/mL) significantly inhibited the TNF-alpha secretion by astrocytes stimulated with lipopolysaccharide (LPS) and substance P (SP). Interleukin-1 (IL-1) has been shown to elevate TNF-alpha secretion from LPS-stimulated astrocytes while having no effect on astrocytes in the absence of LPS. We therefore examined whether IL-1 mediated inhibition of TNF-alpha secretion from primary astrocytes by SSGP. Treatment with SSGP (10-1,000 microg/mL) to astrocytes stimulated with both LPS and SP decreased IL-1 secretion significantly. Moreover, the secretion of TNF-alpha by LPS and SP in astrocytes was progressively inhibited with an increasing amount of IL-1 neutralizing antibody. Our results suggest that SSGP may inhibit TNF-alpha secretion by inhibiting IL-1 secretion and that SSGP has an antiinflammatory activity in the CNS.

show abstract

Inhibitory effect of Asparagus cochinchinensis on tumor necrosis factor-alpha secretion from astrocytes

Cited by 43 publications

References 26 publications

INHIBITORY EFFECT OF TUMOR NECROSIS FACTOR-α SECRETION FROM RAT ASTROCYTES BY CHILBOKEUM

INHIBITORY EFFECT OF TUMOR NECROSIS FACTOR-α SECRETION FROM RAT ASTROCYTES BY CHILBOKEUM

Ethanol Extract from Asparagus Cochinchinensis Attenuates Glutamate-Induced Oxidative Toxicity in HT22 Hippocampal Cells

SunghyangJungki‐San Ga Pogongyoung inhibits IL‐1 mediated tumour necrosis factor‐α secretion in astrocytes

Contact Info

Product

Resources

About