Inhibitory effect of aqueous extract of Cuminum cyminum L. seed on degranulation of RBL-2H3 cells and passive cutaneous anaphylaxis reaction in mice

Abstract

Cuminum cyminum L. (cumin) seed is used as a spice in various countries. Although several functions of the components in cumin seed have been reported, the anti-allergic effect of the water-soluble component in cumin seed has not been reported yet. In this study, we focused on the suppressive effect of cumin seed aqueous extract on degranulation in order to reveal the anti-allergic effect of cumin. Cumin seed aqueous extract significantly suppressed the antigeninduced degranulation of rat basophilic leukemia cell line RBL-2H3 cells in a dose-dependent manner without cytotoxicity. The extract also inhibited the elevation of the intracellular calcium ion concentration induced by antigen. Immunoblot analysis revealed that the extract suppresses phosphorylation of phosphatidylinositol 3-kinase, Bruton's tyrosine kinase, phospholipase C-c1/2, and Akt in the signaling pathways activated by antigen induction via FceRI. Furthermore, the extract suppressed microtubule formation induced by antigen. In addition, oral administration of cumin seed aqueous extract significantly suppressed the passive cutaneous anaphylaxis reaction in BALB/c mice. Our findings suggest that cumin seed contains water-soluble components with the antiallergic effect. Therefore, cumin seed has potential as anti-allergic functional food.

Evaluation of the Effects of Cuminum cyminum on Cellular Viability, Osteogenic Differentiation and Mineralization of Human Bone Marrow-Derived Stem Cells

“…The dosage may affect the effects of cumin on various situations [ 33 ]. In a previous study, 100 to 10,000 μg/mL of aqueous extract of ultra-filtrated cumin seed was applied for the evaluation of degranulation and cell viability of rat basophilic leukemia cells [ 34 ]. The obtained minimum inhibitory concentration for cumin was 2–4 µL/mL (mean: 3.1 µL/mL) [ 8 ].…”

“…Various extraction methods have been used for cumin including extraction methods using water, methanol, ethanol and ethyl acetate [ 34 , 36 , 37 , 38 , 39 ]. An aqueous extract of cumin was applied for the evaluation of the inflammatory response in immune cells and antiallergic effects [ 34 , 40 ]. A methanol extract of cumin was used for the evaluation of the protective role of cumin against biomolecular damage and neuropharmacological activities [ 36 , 39 ].…”

Onycholysis is not a negligible malaise especially in diabetics and people suffering from psoriasis: a dignified way to treat the concern with cuminum and cardamom

“…Cuminum cyminum extract (extremely rich in p-cymene) (0,4%, that corresponds to the maximum percentage admitted by Annex III) [7].…”

Anti-allergic actions of a Chinese patent medicine, huoxiangzhengqi oral liquid, in RBL-2H3 cells and in mice

“…To determine the solid content of HXZQ-OL, 10 mL sample was placed in a 25 mL evaporating dish, followed by evaporating with water bath, and then was dried at 105 C for 3 h. The solid content of HXZQ-OL was 87.9 mg/mL. PCA PCA was conducted following the previous method with modification (Hada et al 2019). HXZQ-OL at dosages of 263.8, 527.6 and 1055 mg/kg that was equivalent to 1/2, 1, 2 times the adult clinical dosage were orally administrated for seven consecutive days.…”