Inhibitory effect of adenovirus-uteroglobin transduction on the growth of lung cancer cell lines

Abstract

Uteroglobin is a secretory protein synthesized by most epithelia, including the respiratory tract. It has strong anti-inflammatory properties that appear to be related to the inhibition of phospholipase A2. Recent experimental evidence indicates that uteroglobin has an inhibitory effect on the proliferation and invasion of cancer cells. We investigated the effects of the adenovirusuteroglobin (ad-UG) transduction on the growth of lung cancer cell lines, which did not express the uteroglobin gene. Upon transduction of ad-UG, the rate of cell growth and the ability to produce colonies in soft agar were evaluated. Cell cycle analysis, Western blot for cell cycle-related proteins and annexin V staining for apoptosis were carried out to see if they were associated with the changes in cell growth. All the tested lung cancer cell lines did not express the uteroglobin gene. The growth rates, and colonyforming ability of transformed cells, were significantly inhibited by the induction of uteroglobin gene expression. The DNA histogram showed that the cell fraction of the G2/M phase was increased, and this G2/M phase arrest was related to a decrease of cdk1 and cyclin A. However, a fraction of apoptotic cells were same as the control. From these results, uteroglobin is thought to have an inhibitory effect on the growth of lung cancer cells. This suggests a potential role for uteroglobin in gene therapy for lung cancer.

Differentially expressed genes from the glioblastoma cell line SHG-44 treated with all-trans retinoic acid in vitro

“…23 Using adenovirus-uteroglobin (ad-UG) transducted lung cancer cell lines, which did not express the UGB gene, it has been shown that growth rates and colony-forming ability of the transformed cells were significantly reduced by the induction of UGB gene expression. 24 To our knowledge, there have been no previous reports regarding UGB expression changes in GBM. Mammaglobin, another member of the UGB family, attracted wide attention because of its potential as a clinical marker for breast metastases.…”

Linguistic recognition system for identification of some possible genes mediating the development of lung adenocarcinoma

“…It has been found that some of these genes were already found to be responsible for lung adenocarcinoma. For example, genes IGFBP3 [5,23], SFTPA1 [18,41], SFTPA2 [41], TNFAIP3 [12], TNFSF6 [4], TNFSF10 [43], UGB [54,24,22], HBB [31] were already found by some earlier investigations. We now describe, in brief, a few genes that are found to be responsible by the two methods.…”

The immune modulation of Clara cell-10 in human peripheral monocytes and dendritic cells

“…The adenoviral construct (Ad-CC-10) is an E1 deleted, replication-deficient adenoviral type 5 vector (Ad5) encoding human CC-10 cDNA. The recombinant Ad-CC-10 was constructed as described previously (26). Briefly, the cDNA of human CC-10 was cloned into the adenoviral shuttle vector, pACCMVpLpA.…”

“…Transduction was accomplished using the standard method (2 h incubation at 37˚C), as described previously (26). The production of the CC-10 protein was assessed by ELISA specific for human CC-10, and a higher concentration was demonstrated with increasing MOI, showing a 10-fold increase in CC-10 protein secretion with 50 MOI and a 15-fold increase with 100 MOI compared with the negative control (Fig.…”

“…In addition, the incidence of cancer in CC-10 knockout mice is extremely high. Induced expression of CC-10 in some cancer cells appears to cause the loss of their transformed phenotype and the suppression of metastasis (26). Overall, the loss of the CC-10 gene expression may be a common characteristic of cancer cells, and CC-10 may act as a tumor suppressor.…”