Inhibitory effect of a Brazilian marine brown alga Spatoglossum schröederi on biological activities of Lachesis muta snake venom

Domingos, Thaisa Francielle Souza; Ortiz-Ramírez, Fredy A.; Villaça, Roberto Campos; Cavalcanti, Diana Negrão; Sanchez, Eládio F.; Teixeira, Valéria Laneuville; Fuly, André Lopes

doi:10.1590/s0102-695x2012005000049

Cited by 5 publications

(5 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Metalloproteases and serine proteases are responsible for such harmful effects. Data from the literature state that plants or their products, mainly those enriched in phenolic compounds [ 29 ], inhibit hemorrhage by chelating metal ions that are essential for the action of metalloproteases and serine proteases [ 9 , 30 , 31 ]. Other plants constituents, as flavonoids, xanthenes, and terpenoids, may also bind to the catalytic site of enzymes and, in turn, inhibit their enzymatic activity [ 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…Polar and nonpolar secondary metabolites have been described as responsible for the inhibition of PLA 2 in snake venom [ 39 ]. Besides plants, other natural products are inhibitors of PLA 2 enzymes, such as diterpenes from the marine brown algae Spatoglossum schröederi [ 31 ] or Canistrocarpus cervicornis [ 40 ]. In addition, these algae also inhibited clotting and hemorrhagic activities induced by L. muta venom [ 31 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…Besides plants, other natural products are inhibitors of PLA 2 enzymes, such as diterpenes from the marine brown algae Spatoglossum schröederi [ 31 ] or Canistrocarpus cervicornis [ 40 ]. In addition, these algae also inhibited clotting and hemorrhagic activities induced by L. muta venom [ 31 , 40 ]. Proteolysis, edema, and hemorrhage contribute to and/or are responsible for the local effects observed in victims after snakebite, which lead to amputation or morbidity in victims.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Inhibitory Effect of PlantManilkara subsericeaagainst Biological Activities ofLachesis mutaSnake Venom

Oliveira

Fernandes

Sanchez

et al. 2014

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Snake venom is composed of a mixture of substances that caused in victims a variety of pathophysiological effects. Besides antivenom, literature has described plants able to inhibit injuries and lethal activities induced by snake venoms. This work describes the inhibitory potential of ethanol, hexane, ethyl acetate, or dichloromethane extracts and fractions from stem and leaves of Manilkara subsericea against in vivo (hemorrhagic and edema) and in vitro (clotting, hemolysis, and proteolysis) activities caused by Lachesis muta venom. All the tested activities were totally or at least partially reduced by M. subsericea. However, when L. muta venom was injected into mice 15 min first or after the materials, hemorrhage and edema were not inhibited. Thus, M. subsericea could be used as antivenom in snakebites of L. muta. And, this work also highlights Brazilian flora as a rich source of molecules with antivenom properties.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Inhibitory Effect of PlantManilkara subsericeaagainst Biological Activities ofLachesis mutaSnake Venom

Oliveira

Fernandes

Sanchez

et al. 2014

BioMed Research International

Self Cite

View full text Add to dashboard Cite

show abstract

“…In contrast, the research on marine algae as inhibitors of toxic effects of snake venoms is limited. Previous results from our group have shown the inhibitory activity of plants, marine sponges and seaweed against snake venoms, but little is known about antivenom properties of algae such as P. brasiliense [ 28 , 43 - 45 ]. An initial study was conducted by Claudino et al [ 34 ] analyzing the inhibitory action of P. brasiliense extract against the toxic effects of L. muta snake venom.…”

Section: Discussionmentioning

confidence: 99%

The red seaweed Plocamium brasiliense shows anti-snake venom toxic effects

Silva¹,

Domingos²,

Fonseca³

et al. 2015

J Venom Anim Toxins incl Trop Dis

View full text Add to dashboard Cite

BackgroundSnakebite is considered a neglected tropical disease by the World Health Organization. In Brazil, about 70% of the envenomation cases are caused by Bothrops snakes. Its venom may provoke hemorrhage, pain, necrosis, hemolysis, renal or cardiac failure and even death in victims. Since commercial antivenom does not efficiently neutralize the local toxic effects of venoms, natural products have been tested in order to provide alternative or complementary treatment to serum therapy. Therefore, the present study aimed to evaluate the ability of the seaweed Plocamium brasiliense and its active derivatives to neutralize hemorrhagic, edematogenic, hemolytic, coagulant and proteolytic activities of B. jararaca venom.MethodsSpecimens of P. brasiliense were collected in Rio de Janeiro state, Brazil, dried and submitted to oil extraction using four solvents of increasing polarities, n-hexane (HEX), dichloromethane (DCM), ethyl acetate (ETA) and hydroalcoholic solution (HYD). The solvents were evaporated, yielding HEX, DCM, ETA and HYD extracts. Further, all extracts were dissolved in dimethylsulfoxide. In addition, two monoterpenes (8-bromo-3,4,7-trichloro-3,7-dimethyl-1E, 5E-octadiene and 1,8-dibromo-3,4,7-trichloro-3,7-dimethyl-1E, 5E-octadiene) and a cholesterol fraction were isolated from the extract of P. brasiliense prepared in hexane. Algal samples were incubated for 30 minutes with B. jararaca venom, and then tested for lethality; hemorrhagic, edematogenic, hemolytic, coagulant and proteolytic effects.ResultsMost of the algal extracts inhibited the toxic effects with different potencies. The DCM extract was the most effective, since it inhibited all types of toxic activity. On the other hand, the HYD extract failed to inhibit any effect. Moreover, the isolated products inhibited proteolysis and protected mice from hemorrhage in 30% of the cases, whereas 8-bromo-3,4,7-trichloro-3,7-dimethyl-1E, 5E-octadiene inhibited 100% and 20% of the hemorrhagic and proteolytic activities, respectively. None of the algal products were toxic to mice.ConclusionSeaweeds may be a promising source of inhibitors against toxic effects caused by B. jararaca envenomation, which may contribute to antivenom treatment.

show abstract

“…Extracts from plants and other natural sources (as those from marine organisms) have been tested for their ability to neutralize a variety of biological and toxic effects of snake venoms. Various pharmacologically active molecules have been identified, and many effects have already been listed for them [9–12], including their antivenom ability [13, 14]. Nowadays, many new bioprospecting approaches are being investigated.…”

Section: Introductionmentioning

confidence: 99%

Antivenom Effects of 1,2,3-Triazoles againstBothrops jararacaandLachesis mutaSnakes

Domingos

Moura

Campos

et al. 2013

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Snake venoms are complex mixtures of proteins of both enzymes and nonenzymes, which are responsible for producing several biological effects. Human envenomation by snake bites particularly those of the viperid family induces a complex pathophysiological picture characterized by spectacular changes in hemostasis and frequently hemorrhage is also seen. The present work reports the ability of six of a series of 1,2,3-triazole derivatives to inhibit some pharmacological effects caused by the venoms of Bothrops jararaca and Lachesis muta. In vitro assays showed that these compounds were impaired in a concentration-dependent manner, the fibrinogen or plasma clotting, hemolysis, and proteolysis produced by both venoms. Moreover, these compounds inhibited biological effects in vivo as well. Mice treated with these compounds were fully protected from hemorrhagic lesions caused by such venoms. But, only the B. jararaca edema-inducing activity was neutralized by the triazoles. So the inhibitory effect of triazoles derivatives against some in vitro and in vivo biological assays of snake venoms points to promising aspects that may indicate them as molecular models to improve the production of effective antivenom or to complement antivenom neutralization, especially the local pathological effects, which are partially neutralized by antivenoms.

show abstract

Inhibitory effect of a Brazilian marine brown alga Spatoglossum schröederi on biological activities of Lachesis muta snake venom

Cited by 5 publications

References 34 publications

Inhibitory Effect of PlantManilkara subsericeaagainst Biological Activities ofLachesis mutaSnake Venom

Inhibitory Effect of PlantManilkara subsericeaagainst Biological Activities ofLachesis mutaSnake Venom

The red seaweed Plocamium brasiliense shows anti-snake venom toxic effects

Antivenom Effects of 1,2,3-Triazoles againstBothrops jararacaandLachesis mutaSnakes

Contact Info

Product

Resources

About