Inhibitory and excitatory synaptic neuroadaptations in the diazepam tolerant brain

Lorenz-Guertin, Joshua M.; Povysheva, Nadezhda V.; Chapman, Caitlyn A.; MacDonald, Matthew L.; Fazzari, Marco; Nigam, Aparna; Nuwer, Jessica L.; Das, Sabyasachi; Brady, Megan L.; Vajn, Katarina; Bambino, Matthew J.; Weintraub, Susan T.; Johnson, Jon W.; Jacob, Tija C.

doi:10.1016/j.nbd.2023.106248

Cited by 3 publications

(1 citation statement)

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“…In particular, the 5-HT1 subfamily is emerging as a significant target for therapeutic drugs in conditions such as insomnia [ 37 ]. GABA is an inhibitory neurotransmitter associated with CNS, whereas GABAAR is closely associated with symptoms of insomnia [ 38 ]. Expression levels of BDNF, a brain-derived neurotrophic factor, correlate with the trophic status of brain neurons and can be used to evaluate insomnia, memory impairment, and other CNS-related diseases [ 15 ].…”

Section: Resultsmentioning

confidence: 99%

Comparative Analysis of the Sedative and Hypnotic Effects among Various Parts of Zizyphus spinosus Hu and Their Chemical Analysis

Li,

Yang,

Song

et al. 2024

Pharmaceuticals

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Zizyphus spinosus Hu (ZS), as a “medicinal and food-homologous” plant, has been used for a long history. The study was to assess the sedative and hypnotic effects among various parts of ZS. The model, diazepam (DZP), ZS kernel (ZSS), ZS flesh (ZSF), and ZS husk (ZSKS) group occurred subsequent to the successful establishment of the para-chlorophenylalanine induced insomnia model via intraperitoneal injection. The latency and duration of sleep in mice in each group were recorded. The substance basis of various parts of ZS was analyzed by the UPLC-QTOF-MS technique. The results showed that relative to the model group, DZP, ZSS, ZSF, and ZSKS groups demonstrated shortened sleep latency (p < 0.05) and extended sleep duration (p < 0.01). The GABA, 5-HT, and BDNF levels were significantly upregulated in the brain tissues of the mice in the DZP, ZSF, and ZSS groups (p < 0.01). However, the improvement in ZSKS was non-significant. Additionally, the mRNA and protein expression levels of 5-HT1AR, GABAARα1, and BDNF in mice in the DZP, ZSS, and ZSF groups were significantly enhanced (p < 0.01). However, the improvement in the ZSKS group was insignificant (p < 0.05). The examination of the substance composition across different parts revealed that the shared chemical basis contributing to the sedative and hypnotic potency of different parts of ZS may involve the presence of compounds such as (1) magnoflorine, (8) betulinic acid, (9) ceanothic acid, and (10) alphitolic acid. It provides a basis for further elucidation of the substance basis responsible for the functional and medicinal effects of ZS.

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Section: Resultsmentioning

confidence: 99%