Inhibitory and cytotoxic activities of chrysin on human breast adenocarcinoma cells by induction of apoptosis

Abstract

Objectives:Chrysin, an active natural bioflavonoid found in honey and many plant extracts, was first known for its antioxidant and anti-inflammatory effects. The fact that antioxidants have several inhibitory effects against different diseases, such as cancer, led to search for food rich in antioxidants. In this study, we investigated the antiproliferative and apoptotic effects of chrysin on the cultured human breast cancer cells (MCF-7).Materials and Methods:Cells were cultured in Roswell Park Memorial Institute medium and treated with different chrysin concentrations for three consecutive days. Cell viability was quantitated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The percentage of apoptotic cells was determined by flow cytometry using Annexin V-fluorescein isothiocyanate.Results:The MTT assay showed that chrysin had an antiproliferative effect on MCF-7 cells in a dose- and time-dependent manner. The 50% cell growth inhibition values for chrysin against MCF-7 cells were 19.5 and 9.2 μM after 48 and 72 h, respectively. Chrysin induced apoptosis in MCF-7 cells as determined by flow cytometry. Chrysin inhibits the growth of the breast cancer cells by inducing cancer cell apoptosis which may, in part, explain its anticancer activity.Conclusion:This study shows that chrysin could also be considered as a promising chemotherapeutic agent and anticancer activity in treatment of the breast cancer cells in future.SUMMARY Chrysin had an antiproliferative effect on human breast cancer cells (MCF-7) cells in a dose- and time-dependent mannerChrysin induced apoptosis in MCF-7 cells, as determined by flow cytometryChrysin inhibits the growth of the breast cancer cells by inducing cancer cell apoptosisChrysin may have anticancer activity. Abbreviations used: Human breast cancer cells (MCF-7), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), phosphate-buffered saline (PBS), normal fibroblast mouse (L929).

The Neuroprotective Effects of Thymoquinone: A Review

“…51 Activated NF-κB induces the pro-inflammatory cytokines, 52 - 54 such as iNOS, 55 COX-2, and microsomal prostaglandin E synthase-1. 56 In addition, inflammation increases cellular reactive oxygen species production by releasing various NF-κB-mediated pro-inflammatory mediators. 57 Therefore, inhibition of microglial activation may be effective for neuronal cell survival.…”

Organophosphorus Compounds and MAPK Signaling Pathways

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Antineoplastic Activity of Chrysin against Human Hepatocellular Carcinoma: New Insight on GPC3/SULF2 Axis and lncRNA-AF085935 Expression

“…One of these natural flavonoids is chrysin, with the chemical structure of 5,7-dihydroxyflavone, which is present in propolis and honey [ 14 ]. The chrysin antitumor activity was documented in various types of human cancer cell lines, including gastric [ 15 ], breast [ 16 ], colorectal [ 17 ], and prostate [ 18 ] cancer cells due to its ability to induce cell apoptosis and cell cycle arrest [ 19 ]. Interestingly, previous studies showed the antitumor potential of chrysin against HCC through p53/Bcl-2/caspase-9 pathway activation [ 20 ] and targeting hexokinase-2 [ 12 ].…”