Inhibitory Activity of Yokukansankachimpihange against Nerve Growth Factor-Induced Neurite Growth in Cultured Rat Dorsal Root Ganglion Neurons

Murayama, Chiaki; Watanabe, Shimpei; Nakamura, Motokazu; Norimoto, Hisayoshi

doi:10.3390/molecules200814959

Cited by 8 publications

(7 citation statements)

References 22 publications

(31 reference statements)

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“…The Uncaria alkaloids rhynchophylline and isorhynchophylline did not alter neurite outgrowth (Figure A and D). Rhynchophylline is even known to protect cells against NGF‐induced outgrowth in Dawley rat neurons …”

Section: Resultsmentioning

confidence: 83%

Enantioselective Organocatalytic Synthesis of a Secoyohimbane‐Inspired Compound Collection with Neuritogenic Activity

et al. 2017

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Natural products provide evolutionary validated core structures to inspire the synthesis of new compound collections endowed with neurite growth-promoting activity. Rhynchophylline is the major component of Uncaria species, and has been used to treat neurological diseases in Chinese traditional medicine. Based on the structure of this spirocyclic secoyohimbane alkaloid, we developed a highly enantioselective and efficient organocatalyzed synthesis method to provide a tetracyclic secoyohimbane scaffold incorporating a quaternary and three tertiary stereogenic centers, in a one-pot multistep reaction sequence. A compound collection of derived secoyohimbanes was synthesized and expanded by decorating the periphery of the basic scaffold with additional substituents to increase the diversity. Evaluation of the different subcollections of secoyohimbanes for modulation of neurite outgrowth in the SH-SY5Y human cell line led to the discovery of new compounds that promote neurite outgrowth.

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Section: Resultsmentioning

confidence: 83%

Enantioselective Organocatalytic Synthesis of a Secoyohimbane‐Inspired Compound Collection with Neuritogenic Activity

et al. 2017

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“…We previously reported that YKSCH inhibited NGF-induced reductions in neurite growth in cultured rat DRG neurons [8]. However, increases were observed in NGF concentrations after the repeated application of SDS [18].…”

Section: Discussionmentioning

confidence: 92%

“…YKSCH and some of its ingredients were found to exert inhibitory effects against NGF-induced neurite growth in cultured rat dorsal ganglion (DRG) neurons [8]. A previous study demonstrated that local NGF concentrations were lower in normal skin than in the lesional skin of AD patients; therefore, it may be one of the main mediators of the density of cutaneous innervation [9].…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects and Potential Mechanism of Yokukansankachimpihange on SDS-induced Itch-associated Responses in Mice

Zhang¹,

Imamura²,

Yoshida³

et al. 2021

JDDMC

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Yokukansankachimpihange (YKSCH) is a Chinese formula proven in Japan to be an effective treatment for pruritus in patients with atopic dermatitis or chronic urticaria and dry eczema with psychoneurotic symptoms, such as insomnia. Sodium dodecyl sulfate (SDS) may cause skin irritation in both humans and animals. Adverse effects, such as skin dryness, barrier destruction, dermatitis, and pruritus, developed following the repeated application of 10% SDS to a local site. Therefore, the present study investigated the antipruritic effects of YKSCH on skin irritation induced by a repeated SDS stimulation in mice and attempted to elucidate the underlying mechanism of action. ICR mice were stimulated with 10% SDS and co-treated with oral YKSCH extract for four days. Only oral YKSCH extract was administered on the fifth day. Scratching behavior was observed 24 h after the last application of SDS. Intraepidermal nerve growth was investigated by an immunofluorescence analysis. NGF concentrations in the epidermis were measured using an enzyme-linked immunosorbent assay. The expression of amphiregulin (Areg) and semaphorin 3A (Sema3A) was assessed by quantitative real-time PCR. The oral administration of YKSCH (200-400 mg/kg) dose-dependently suppressed pruritus, significantly reduced intraepidermal nerve growth, and down-regulated epidermal Areg mRNA expression in SDS-treated mice. These results suggest that YKSCH exerts antipruritic effects against SDS-induced pruritus in mice. The mechanism of action of YKSCH may involve reductions in intraepidermal nerve density due to the down-regulated expression of Areg.

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“…UGSJB treats aggressive behavior by suppressing excess glutamateric neuron activity in the hippocampus [37], anxiety through the serotonergic neurotransmission pathway [38], chronic pruritus by inhibiting nerve growth factor–induced neurite growth [39], and insomnia by increasing stage 2 sleep and decreasing sleep latency [40]. UGSJB has some neuronal effects and was reported to improve symptoms of ASD in a case report [41].…”

Section: Discussionmentioning

confidence: 99%

Safety, effectiveness, and economic evaluation of an herbal medicine, Ukgansangajinpibanha granule, in children with autism spectrum disorder: a study protocol for a prospective, multicenter, randomized, double-blinded, placebo-controlled, parallel-group clinical trial

Lee

Shin

Kim

et al. 2019

Trials

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Background Autism spectrum disorder (ASD) is characterized by continuous impairment in communication and social interaction and by limited and repetitive behaviors, interests, or activities. Behavioral, educational, and pharmaceutical interventions have been shown to reduce behavioral disabilities, improve verbal/non-verbal communication, and help patients acquire self-reliance skills. However, there has been a lack of systematic verification and consensus regarding the treatment of the core symptoms of ASD because of its unclear etiology. Ukgansangajinpibanha (UGSJB), a legitimately prescribed herbal medicine for nervousness, insomnia, night crying, and malnutrition in South Korea and Japan, has been used for angry, sensitive, nervous, and unsettled children with ASD. Methods/design This trial is a prospective, multicenter, randomized, double-blinded, placebo-controlled, parallel-group, clinical trial. The 4- to 6-year-old children with ASD will be randomly assigned to following groups: A UGSJB granule with acupuncture, twice daily (n = 120) A placebo group with acupuncture, twice daily (n = 120). The following outcome measures will be used: behavior by the Childhood Autism Rating Scale, Autism Behavior Checklist, and Aberrant Behavior Checklist; social maturity by the Social Maturity Scale; quality of life by the Child Health Questionnaire and EuroQoL Five-dimension Five-level Youth; and parental stress by the Parenting Stress Index at baseline and at 6, 12, and 24 weeks after the beginning of treatment. In addition, to evaluate safety, we will investigate the adverse reactions that may be caused by UGSJB granule. Finally, we will make an economic evaluation of UGSJB for the treatment of ASD. Discussion We prepared a well-designed clinical trial to investigate the safety and effectiveness of UGSJB on ASD symptoms compared with placebo treatment. The results from this study will provide clinical evidence on the safety, effectiveness, and economic value of UGSJB combined with acupuncture in children with ASD. Trial registration Clinical Research Information Service: KCT0003007 (registered on April 5, 2018) Electronic supplementary material The online version of this article (10.1186/s13063-019-3537-7) contains supplementary material, which is available to authorized users.

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Inhibitory Activity of Yokukansankachimpihange against Nerve Growth Factor-Induced Neurite Growth in Cultured Rat Dorsal Root Ganglion Neurons

Cited by 8 publications

References 22 publications

Enantioselective Organocatalytic Synthesis of a Secoyohimbane‐Inspired Compound Collection with Neuritogenic Activity

Enantioselective Organocatalytic Synthesis of a Secoyohimbane‐Inspired Compound Collection with Neuritogenic Activity

Inhibitory Effects and Potential Mechanism of Yokukansankachimpihange on SDS-induced Itch-associated Responses in Mice

Safety, effectiveness, and economic evaluation of an herbal medicine, Ukgansangajinpibanha granule, in children with autism spectrum disorder: a study protocol for a prospective, multicenter, randomized, double-blinded, placebo-controlled, parallel-group clinical trial

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