Inhibitory Activity of Vaginal Lactobacilli Against Human Pathogens

Petrova, M. O.; Georgieva, Ralitsa; Dimitonova, Silvia P.; Ivanovska, Nina; Hadjieva, N.; Danova, S.

doi:10.1080/13102818.2009.10818502

Cited by 5 publications

(5 citation statements)

References 13 publications

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“…Based on these results, strains LB6 and LB7 were considered as probiotic candidate strains with strong transit tolerance and biological activity [22][23][24]. The present results are in support of this.…”

Section: Discussionsupporting

confidence: 79%

“…As a source of CFS, 11 Lactobacillus vaginal and dairy-product isolates from the laboratory collection of the Institute of Microbiology were selected on the basis of their recently reported broad-spectrum antibacterial activities [22][23][24]. We collected evidence suggesting that highly diluted noninhibitory amounts of CFS (10 -2 to 10 -4 ) can still have a biofilm-modulation capacity and that this is probably due to factors different from those involved in their antibacterial activity.…”

Section: Introductionmentioning

confidence: 99%

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Modulation of Escherichia coli biofilm growth by cell-free spent cultures from lactobacilli

et al. 2012

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E. coli biofilms cause serious problems in medical practice by contaminating surfaces and indwelling catheters. Due to the rapid development of antibiotic resistance, alternative approaches to biofilm suppression are needed. This study addresses whether products released by antagonistic bacteria — Lactobacillus isolates from vaginal and dairy-product samples could be useful for controlling E. coli biofilms. The effects of diluted cell-free supernatants (CFS) from late-exponential Lactobacillus cultures on the growth and biofilm production of Escherichia coli were tested. Most of the CFS applied as 10−2 had no impact on bacterial growth, biofilm development however was influenced even by 10−4 of CFS. Initial screening by crystal violet assay showed that biofilm modulation varied between different CFS and E. coli combinations from inhibition to activation; however three of the tested CFS showed consistency in biofilm suppression. This was not due to antibacterial activity since Live/Dead fluorescence labeling showed insignificant differences in the amount of dead cells in control and treated samples. Some E. coli strain-specific mechanisms of response to the three CFS included reduction in hydrophobicity and motility. Released exoploysaccharides isolated from the three CFS stimulated sessile growth, but proteinase K reduced their inhibitory activities implying participation of protein or peptide biofilm suppression factor(s).

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Modulation of Escherichia coli biofilm growth by cell-free spent cultures from lactobacilli

et al. 2012

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“…In this study, Lactobacillus jensenii and Lactobacillus fermentum were the most predominant species reported from the isolation and identification processes of LAB isolates from the vagina swab samples. Petrova, et al 27 also reported that the most active strains amongst a preselected twenty vagina LAB isolates from Bulgarian women belonged to the species L. fermentum, L. gasserriand L. salivarus. A study of vaginal lactobacilli strain prevalence of Korean women reported Lactobacilli crispatusas the most prevalent strain 28 .…”

Section: Quantitative Determination Of Antimicrobialmentioning

confidence: 95%

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2014

IJPSR

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Oral probiotics has been found useful in treatment or prevention of urogenital infections. The ability of probiotic organisms to serve as biotherapeutic agents can be attributed to various properties they possess. A total of thirty-two lactic acid bacteria (LAB) strains isolated from the vagina swab samples of healthy women in selected areas in southwestern Nigeria were characterised as Lactobacillus fermentum, Lactobacillus jensenii, Lactobacillus delbrueckii, Lactobacillus plantarum, Lactobacillus acidophilus and Lactobacillus lactis. L. fermentum exhibited a high antagonistic effect with zones of inhibition of 24mm and 25mm against Klebsiella sp. and E. coli respectively. L. jensenii also had a high activity with inhibition zone of 24mm against Klebsiella sp. Percentage antibiotic resistance of the isolates reflects that 86% of the isolates exhibited resistance to both amoxacillin and gentamicin; 73% to ampiclox and 64% to rocephine while low percentage resistance from 0% to 5% was observed for the fluoroquinolones and augumentin. The knowledge of the antimicrobial resistance of exogenously applied probiotic is of interest to predict their behaviour in patients subject to any type of chemotherapy, as well as to consider the concomitant use of the probiotic and antibiotics for the restoration of the normal urogenital flora. Production of lactic acid and hydrogen peroxide was detected in all the LAB isolates and they were observed for up to 96hours. L. fermentum produces more hydrogen peroxide when compared with the other isolates, it had the highest zones of inhibition against genital pathogens, hence, may be considered as an isolate with outstanding probiotic potential. INTRODUCTION: Genital infections in women may result in vaginal discharge, mucosal ulceration producing local discomfort and pain on intercourse.

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“…The antimicrobial activity of LAB strains due to the produced variety of postbiotics is a promising base for food safety. With special attention to the LAB with a broad spectrum of activity, different strains with dairy and/or human origin were characterized [6,7]. The dominance of Lactiplantibacillus plantarum in autochthonous microbiota of traditional Bulgarian dairy products has been pointed out [8].…”

Section: Introductionmentioning

confidence: 99%

Postbiotics Production of Candidate-Probiotic Lactiplantibacillus plantarum AC131 with Renewable Bio Resources

Danova,

Yankov,

Dobreva

et al. 2023

Life

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Lactiplantibacillus plantarum is a versatile specie, well known as a producer of lactic acid (LA) and other metabolites with biotechnological significance. The present work characterizes growth and lactic acid production of the candidate-probiotic strain L. plantarum AC131, from Bulgarian white brined cheeses. Different nutritional media with ingredients from renewable resources—reduced sugars from dried distillers’ grains with soluble (DDGS) and waste waters from the water-vapor distillation of Bulgarian Rosa alba L. and Rosa damascena Mill. essential oil—were assessed. The results obtained showed significant LA production (up to 95% conversion) in modified MRS broth with reducing sugars from DDGS hydrolysates. The addition of R. alba L. and R. damascena Mill. distillation effluents stimulated the growth and biological activity of postbiotics produced by L. plantarum AC131. In both experimental approaches, a statistically significant inhibition (from 20 to 60%) of E. coli HB 101 growth was found during 24 h exposure and a variable effect on the biofilm formed. In conclusion, reducing sugars from DDGS hydrolysates can be successfully used as a carbon source for lactic acid production. In the case of fermentation without pH control, the process is product inhibited, while with pH control, nearly full conversion was achieved. Postbiotics produced during the process of fermentation showed a variety of biological activity and inhibitory effects on the growth of Escherichia coli HB 101.

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Inhibitory Activity of Vaginal Lactobacilli Against Human Pathogens

Cited by 5 publications

References 13 publications

Modulation of Escherichia coli biofilm growth by cell-free spent cultures from lactobacilli

Modulation of Escherichia coli biofilm growth by cell-free spent cultures from lactobacilli

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Postbiotics Production of Candidate-Probiotic Lactiplantibacillus plantarum AC131 with Renewable Bio Resources

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