“…According to the ABTS assay, SSF provided an increase of up to 93% in the AA of the extract (498.46 mgTE/gdw) as compared to the value before SSF (258.18 mgTE/gdw). Ethanolic extract from cascalote was evaluated against DPPH and ABTS antioxidant assays [41]. At 500 mg/L, the inhibition rate was higher than 90% in both cases.…”
Section: Discussionmentioning
confidence: 99%
“…Identified polyphenol molecules in cascalote pods have different biological activities, such as antioxidant effects, hepatoprotective effects [41], anthelmintic effects [39,44], arginase inhibitory activity [45], antimicrobial activity [4,43,46], and gastroprotective effects [40]. These benefits provide a wide range of possible applications in the food, pharmaceutical, and cosmetic industries.…”
Cascalote pods are an important source of phenolic compounds, mainly recovered using solvent extraction methods. To find a sustainable alternative to these methods, this study aimed to evaluate solid-state fermentation (SSF) in order to enhance the extractability of total phenolic compounds (TPC) with antioxidant activity (AA) from cascalote pods. Aspergillus niger GH1 was selected based on the amount of TPC released and AA measured (ABTS, DPPH, FRAP) in a short period of time. Moreover, moisture, temperature, inoculum size, and mineral composition were evaluated. The largest amount of TPC released was 124.17 mg/gdw (g of dry weight) after 12 h of culture, which significantly correlated with the AA (Pearson’s R = 0.94). Moisture and KH2PO4 concentration were the main influencing factors of TPC release. Treatment 6 (1 × 107 spores/gdw, 30 °C, 60% moisture, mineral composition (g/L): KH2PO4, 1.52; NaNO3, 7.65; and MgSO4, 1.52) was selected due to the highest values of both TPC and AA. SSF-assisted extraction allowed for an increase of 118% and 93% in TPC and AA values, respectively. Corilagin, lagerstannin, geraniin, and ellagic acid were the main phenolic compounds identified by RP-HPLC-ESI-MS in the cascalote extracts. The results obtained demonstrate the feasibility of SSF-assisted extraction as a biotechnological alternative for the recovery of important bioactive molecules from this underutilized material.
“…According to the ABTS assay, SSF provided an increase of up to 93% in the AA of the extract (498.46 mgTE/gdw) as compared to the value before SSF (258.18 mgTE/gdw). Ethanolic extract from cascalote was evaluated against DPPH and ABTS antioxidant assays [41]. At 500 mg/L, the inhibition rate was higher than 90% in both cases.…”
Section: Discussionmentioning
confidence: 99%
“…Identified polyphenol molecules in cascalote pods have different biological activities, such as antioxidant effects, hepatoprotective effects [41], anthelmintic effects [39,44], arginase inhibitory activity [45], antimicrobial activity [4,43,46], and gastroprotective effects [40]. These benefits provide a wide range of possible applications in the food, pharmaceutical, and cosmetic industries.…”
Cascalote pods are an important source of phenolic compounds, mainly recovered using solvent extraction methods. To find a sustainable alternative to these methods, this study aimed to evaluate solid-state fermentation (SSF) in order to enhance the extractability of total phenolic compounds (TPC) with antioxidant activity (AA) from cascalote pods. Aspergillus niger GH1 was selected based on the amount of TPC released and AA measured (ABTS, DPPH, FRAP) in a short period of time. Moreover, moisture, temperature, inoculum size, and mineral composition were evaluated. The largest amount of TPC released was 124.17 mg/gdw (g of dry weight) after 12 h of culture, which significantly correlated with the AA (Pearson’s R = 0.94). Moisture and KH2PO4 concentration were the main influencing factors of TPC release. Treatment 6 (1 × 107 spores/gdw, 30 °C, 60% moisture, mineral composition (g/L): KH2PO4, 1.52; NaNO3, 7.65; and MgSO4, 1.52) was selected due to the highest values of both TPC and AA. SSF-assisted extraction allowed for an increase of 118% and 93% in TPC and AA values, respectively. Corilagin, lagerstannin, geraniin, and ellagic acid were the main phenolic compounds identified by RP-HPLC-ESI-MS in the cascalote extracts. The results obtained demonstrate the feasibility of SSF-assisted extraction as a biotechnological alternative for the recovery of important bioactive molecules from this underutilized material.
“…Para comprender esta relación, revisaremos algunos conceptos básicos de proteómica, sus métodos y nuestra experiencia en la aplicación de estos en 4 casos de estudios. En el primero, describiremos como el extracto etanólico de los frutos de Caesalpinia coriaria Jacq inhiben la oxidación de proteínas hepáticas de rata como parte de sus mecanismos antioxidantes y hepatoprotectores [2]. Luego, nos referiremos a los efectos pro-oxidantes del extracto etanólico de semillas de Tabenaemontana cymosa sobre las proteínas del tracto digestivo de las larvas del mosquito Aedes aegypti como parte de su mecanismo de acción larvicida [3].…”
Section: Introductionunclassified
“…To understand this relationship, we will review some basic concepts of proteomics, its methods and our experience in 4 study cases. In the first, we will describe how the ethanolic extract of the fruits of Caesalpinia coriaria Jacq inhibits the oxidation of rat liver proteins as part of its antioxidant and hepatoprotective mechanisms [2]. Then, we will refer to the prooxidant effects of the ethanolic extract of Tabenaemontana cymosa seeds on the digestive tract proteins of the Aedes aegypti larvae mosquito as part of its larvicidal action mechanism [3].…”
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