Inhibitory activity of polysaccharide extracts from three kinds of edible fungi on proliferation of human hepatoma SMMC-7721 cell and mouse implanted S180 tumor

Abstract

AIM:To determine the activities of polysaccharide extracts from Flammulina velutipes (Curt. ex Fr.) Sing (FV), Lentinus edodes (LE)and Agaricus bispo-rus Sing (AB) on the proliferation of human hepatoma SMMC-7721 cells in vitro and on mouse implanted S-180 tumors in vivo.METHODS:The polysaccharide extracts were isolated from the fruit bodies of FV, LE and AB by the methods of hot-water extraction,Sevag's removal of proteins, ethanol precipitation, trypsin digestion and ethanol fractional precipitation. Human hepatoma SMMC-7721 cells were treated with 50mg/L polysaccharide extracts, and the mitosis index, mitochondria activity and cell proliferation were detected at different times in both control and experimental groups. The mice with S-180 implanted tumors were injected with the polysaccharide extracts at 24mg/kg body weight for 9d and the tumor weight was measured on the 15th day.RESULTS:The mitosis index of hepatoma cells in vitro could be significantly decreased by treatment with the polysaccharide extracts from the three kinds of edible fungi (P< 0.005). The cell numbers and mitochondria activity of SMMC-7721 cells treated with polysaccharide extracts were lower than those in control groups (P<0.005). The inhibition rates of polysaccharide extracts against implanted S-180 tumors in mice were 52.8%, 56.6% and 51.9% respectively compared with that in control groups.CONCLUSION:The polysaccharide extracts from the three kinds of edible fungi could inhibit not only the cultured malignant cells in vitro but also implanted S-180 tumor in vivo.

Golden Needle Mushroom: A Culinary Medicine with Evidenced-Based Biological Activities and Health Promoting Properties

“…The polysaccharides of F. velutipes demonstrated antitumor and anticancer properties against sarcoma SC-180 mouse model and S-180 mice tumor model in vivo and hepatoma SMMC-7721 cells in vitro (Leung et al, 1997; Jiang et al, 1999). Recently, crude polysaccharide from mycelia of the mushroom was also found to reduce the proliferation of human BEL-7402 lung cancer cells by 45% at 640 μg/mL (Zhao et al, 2013).…”

Effect of Brefeldin A on cell-wall polysaccharide production in the red microalga Porphyridium sp. (Rhodophyta) through its effect on the Golgi apparatus

“…Soluble polysaccharide Ethanol in a ratio of two volumes to one volume of supernatant was used to precipitate the soluble polysaccharide from the supernatant (Jiang et al 1999;Li et al 2001). For the pulse-chase experiments, free 14 C was purged from the soluble polysaccharide complex by six cycles of redissolving the soluble polysaccharide complex in doubly distilled water (pH 4.5) and precipitating it with ethanol.…”

Influência de Agaricus blazei Murrill sobre o tumor sólido de Ehrlich e linfonodos poplíteos de camundongos

“…Os valores médios de AgNORs foram semelhantes (P>0,05) entre os grupos (Tab.1), indicando que a administração do extrato aquoso de ABM não interferiu na atividade nuclear e conseqüente potencial proliferativo do TSE, diferentes do observado por Jiang et al (1999) …”

“…Foi demonstrado in vitro que o extrato total e os polissacarídeos isolados do cogumelo brasileiro Agaricus blazei Murrill (ABM) possuem ação antitumoral direta sobre o sarcoma 180 e, in vivo, diminuem sua implantação (Itho et al, 1997;Jiang et al, 1999;Lee et al, 2003).…”

“…Cobaias que receberam o extrato de Agaricus blazei via oral apresentaram aumento do número e da atividade de células natural killers (NK) e aumento nas titulações de IgM, IgG e interleucina -1β (Ohno et al, 2001). …”