Inhibitory activity of lupinifolin isolated from Derris reticulata stem against biofilm formation of Streptococcus mutans and Staphylococcus aureus

Pulbutr, Pawitra; Thongrak, Kaewkallaya; Thitprapai, Apichart; Rattanakiat, Sakulrat; Mudjupa, Chawannuch; Jaruchotikamol, Achida

doi:10.4103/pr.pr_57_20

Cited by 3 publications

(6 citation statements)

References 28 publications

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“…Studies have suggested that Lup disrupts the cell walls and membranes of Gram-positive microorganisms, thus increasing permeability and decreasing membrane potential, which eventually leads to cell leakage. 30,55,66 Moreover, it has demonstrated antibiofilm activity and improved methicillinresistant S. aureus (MRSA) sensitivity to ampicillin and cloxacillin, 30,31,67 thus possibly having a synergistic effect by targeting a variety of cell mechanisms other than the cell wall and membrane, which is a common feature among flavonoids. 52,68 It will be necessary to investigate whether PreA accumulates in the mycobacterial cell wall and the cytosol, as hypothesized for Caj and Lup, given that flavonoids are able to target a wide variety of molecules, thereby having an impact at multiple levels.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Antimycobacterial Precatorin A Flavonoid Displays Antibiofilm Activity against Mycobacterium bovis BCG

Niño-Padilla,

Espitia,

Velazquez

et al. 2023

ACS Omega

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The aim of this study was to evaluate the potential antibiofilm activity of Rhynchosia precatoria (R. precatoria) compounds over Mycobacterium bovis BCG (M. bovis BCG) as a model for Mycobacterium tuberculosis (Mtb). We evaluated the antibiofilm activity as the ability to both inhibit biofilm formation and disrupt preformed biofilms (bactericidal) of R. precatoria compounds, which have been previously described as being antimycobacterials against Mtb. M. bovis BCG developed air−liquid interface biofilms with surface attachment ability and drug tolerance. Of the R. precatoria extracts and compounds that were tested, precatorin A (PreA) displayed the best biofilm inhibitory activity, as evaluated by biofilm biomass quantification, viable cell count, and confocal and atomic force microscopy procedures. Furthermore, its combination with isoniazid at subinhibitory concentrations inhibited M. bovis BCG biofilm formation. Nonetheless, neither PreA nor the extract showed bactericidal effects. PreA is the R. precatoria compound responsible for biofilm inhibitory activity against M. bovis BCG.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Antimycobacterial Precatorin A Flavonoid Displays Antibiofilm Activity against Mycobacterium bovis BCG

Niño-Padilla,

Espitia,

Velazquez

et al. 2023

ACS Omega

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“…The previous study recently reported that the combinations of lupinifolin with ampicillin or cloxacillin produced synergistic effects against MSSA and MRSA with a FICI of <0.5 14 . Lupinifolin at its sub-MICs significantly exhibited inhibitory actions against biofilm formation of S. mutans, E. faecalis, E. faecium and MSSA 11,13 . However, the inhibitory action of Lupinifolin against MRSA biofilm formation has not been established.…”

Section: Derris Reticulatamentioning

confidence: 97%

“…(Leguminosae-Papilionoideae). It has been demonstrated that lupinifolin possesses antibacterial activity against certain gram-positive cocci including Streptococcus mutans, Enterococcus faecalis, Enterococcus faecium, MSSA as well as MRSA [7][8][9][10][11][12][13][14] . Lupinifolin was found to exert its antibacterial activity via disruption of bacterial cell membrane structure and functions [9][10] .…”

Section: Derris Reticulatamentioning

confidence: 99%

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Antibiofilm Formation Activity of Lupinifolin Against Methicillin-Resistant Staphylococcus aureus

Rattanakia¹,

Taensantia²,

Jaemamporn³

et al. 2022

Pakistan J. of Biological Sciences

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Background and Objective: Biofilm formation activity of Methicillin-resistant Staphylococcus aureus (MRSA) is one of the crucial factors rendering this pathogenic bacterium difficult to be eradicated. It has been reported that lupinifolin, is a major phytochemical agent isolated from Derris reticulata Craib. stem possesses antibacterial activity against MRSA. This study aimed to investigate the effects of lupinifolin and its combinations with some antibacterial drugs, including ampicillin, cloxacillin or vancomycin, on the biofilm formation activity of MRSA. Materials and Methods: The crystal violet biofilm formation assay was performed to evaluate the biofilm formation activity. Results: Lupinifolin produced a significant inhibitory activity against MRSA biofilm formation with the median inhibitory concentration (IC 50 ) of 7.96±3.05 µg mLG 1 (n = 6) at 24 hrs incubation. Lupinifolin at the concentrations of sub-MICs (1, 2, 4 and 8 µg mLG 1 ) combined with the antibacterial drugs at their sub-MICs also exhibited substantial antibiofilm formation activities. The maximal antibiofilm activity was found with the combination of lupinifolin (8 µg mLG 1 ) and vancomycin (1 µg mLG 1 ) by the percentage inhibition of 102.39±0.89 (n = 8). The antibiofilm formation activities of the combinations between lupinifolin and the antibacterial drugs at various concentrations tested were also significantly higher than those of lupinifolin alone. Conclusion: These results indicated that lupinifolin can potentially be developed as an antibacterial enhancer for the management of biofilm-associated bacterial infections caused by MRSA, in which the current pharmacological treatment is still limited.

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Antibiofilm and antibacterial activities of lupinifolin in combination with protein synthesis inhibitors against methicillin-resistant Staphylococcus aureus

Kwaengmuang,

Chaiyawong,

Warong

et al. 2023

J Herbmed Pharmacol

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Introduction: Methicillin-resistant Staphylococcus aureus (MRSA)-derived biofilm formation is a crucial virulence factor, which essentially contributes to therapeutic challenges. This study aims to evaluate the antibiofilm and antibacterial formation activities of lupinifolin, a prenylated flavanone derived from Derris reticulata Craib. stem, in combination with protein synthesis inhibitors. Methods: The crystal violet biofilm formation assay was performed to determine the biofilm formation activity. The synergistic antibacterial activities were evaluated using the checkerboard and time-kill assays. Results: Lupinifolin and tetracycline significantly reduced MRSA biofilm formation with IC50 values of 15.32 ± 5.98 and 13.42 ± 5.90 µg/mL, respectively. On the contrary, the individual treatment of streptomycin and clindamycin tended to enhance biofilm formation. Lupinifolin at the sub-MIC of 8 µg/mL in combination with certain sub-MICs of tetracycline (8 and 16 µg/mL), streptomycin (16, 32, and 64 µg/mL), or clindamycin (4, 8, and 16 µg/mL) caused significant inhibitions against MRSA biofilm formation (P<0.05). The combination of lupinifolin and streptomycin exhibited a synergy (FIC index <0.625), confirmed in the time-kill assay. Conversely, the combination of lupinifolin and tetracycline or clindamycin resulted in no interaction (FIC indices of 1.0078 and <1.0156, respectively). Conclusion: The antibacterial synergy of lupinifolin and streptomycin possibly contributed to their antibiofilm-forming activity. However, the combinations of lupinifolin and tetracycline or clindamycin conceivably executed their antibiofilm activity directly against the MRSA biofilm formation process. These findings indicate a potential role for lupinifolin as an antibiofilm enhancer to diminish MRSA biofilm formation.

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Inhibitory activity of lupinifolin isolated from Derris reticulata stem against biofilm formation of Streptococcus mutans and Staphylococcus aureus

Cited by 3 publications

References 28 publications

Antimycobacterial Precatorin A Flavonoid Displays Antibiofilm Activity against Mycobacterium bovis BCG

Antimycobacterial Precatorin A Flavonoid Displays Antibiofilm Activity against Mycobacterium bovis BCG

Antibiofilm Formation Activity of Lupinifolin Against Methicillin-Resistant Staphylococcus aureus

Antibiofilm and antibacterial activities of lupinifolin in combination with protein synthesis inhibitors against methicillin-resistant Staphylococcus aureus

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