Inhibitory activity of blasticidin A, a strong aflatoxin production inhibitor, on protein synthesis of yeast: selective inhibition of aflatoxin production by protein synthesis inhibitors

Yoshinari, Tomoya; Noda, Yoichi; Yoda, Koji; Sezaki, Hiroshi; Nagasawa, Hiromichi; Sakuda, Shohei

doi:10.1038/ja.2010.36

Cited by 38 publications

(27 citation statements)

References 23 publications

(17 reference statements)

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“…Aspergillus parasiticus NRRL 2999 was used as a producer of aflatoxins B 1 and G 1 throughout the study [19]. Aflatoxins B 1 and G 1 are the main aflatoxins produced by the NRRL 2999 strain.…”

Section: Methodsmentioning

confidence: 99%

Inhibitory Effects of Respiration Inhibitors on Aflatoxin Production

Sakuda

Prabowo

Takagi

et al. 2014

Toxins

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Aflatoxin production inhibitors, which do not inhibit the growth of aflatoxigenic fungi, may be used to control aflatoxin without incurring a rapid spread of resistant strains. A respiration inhibitor that inhibits aflatoxin production was identified during a screening process for natural, aflatoxin-production inhibitors. This prompted us to evaluate respiration inhibitors as potential aflatoxin control agents. The inhibitory activities of four natural inhibitors, seven synthetic miticides, and nine synthetic fungicides were evaluated on aflatoxin production in Aspergillus parasiticus. All of the natural inhibitors (rotenone, siccanin, aptenin A5, and antimycin A) inhibited fungal aflatoxin production with IC50 values around 10 µM. Among the synthetic miticides, pyridaben, fluacrypyrim, and tolfenpyrad exhibited strong inhibitory activities with IC50 values less than 0.2 µM, whereas cyflumetofen did not show significant inhibitory activity. Of the synthetic fungicides, boscalid, pyribencarb, azoxystrobin, pyraclostrobin, and kresoxim-methyl demonstrated strong inhibitory activities, with IC50 values less than 0.5 µM. Fungal growth was not significantly affected by any of the inhibitors tested at concentrations used. There was no correlation observed between the targets of respiration inhibitors (complexes I, II, and III) and their IC50 values for aflatoxin-production inhibitory activity. This study suggests that respiration inhibitors, including commonly used pesticides, are useful for aflatoxin control.

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Section: Methodsmentioning

confidence: 99%

Inhibitory Effects of Respiration Inhibitors on Aflatoxin Production

Sakuda

Prabowo

Takagi

et al. 2014

Toxins

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“…It is a potent inhibitor of AF biosynthesis by A. parasiticus (Sakuda et al, 2000). Using two-dimensional differential gel electrophoresis (2D-DIGE) Yoshinari et al (2010) showed that blasticidin A inhibited AF (total of B 1 and G 1 ) production and fungal growth with IC 50 values of 0.25 and 1.6 µM, respectively. MALDI-TOF MS analysis of protein spots on the 2D-DIGE gel of blasticidin A-treated A. flavus showed decreased amounts of AF biosynthetic enzymes including Vbs, OmtB, OmtA, NorA, Ver-1 and Nor-1 after 36 h treatment.…”

Section: Blasticidins a And S From Streptomyces Griseochromogenesmentioning

confidence: 99%

Aflatoxins: Mechanisms of Inhibition by Antagonistic Plants and Microorganisms

Razzaghi‐Abyaneh¹,

Shams-Ghahfarokhi²,

Chang³

2011

Aflatoxins - Biochemistry and Molecular Biology

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“…Precocene II may affect a regulatory mechanism leading to expression of TRI6 in the trichothecene production pathway in the fungus. To investigate the mode of action of precocene II, proteome analysis by two-dimensional differential gel electrophoresis (2D-DIGE) was performed [14,15]. …”

Section: Resultsmentioning

confidence: 99%

Correlation of ATP Citrate Lyase and Acetyl CoA Levels with Trichothecene Production in Fusarium graminearum

Sakamoto

Tsuyuki

Yoshinari

et al. 2013

Toxins

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Thecorrelation of ATP citrate lyase (ACL) and acetyl CoA levels with trichothecene production in Fusarium graminearum was investigated using an inhibitor (precocene II) and an enhancer (cobalt chloride) of trichothecene production by changing carbon sources in liquid medium. When precocene II (30 µM) was added to inhibit trichothecene production in a trichothecene high-production medium containing sucrose, ACL expression was reduced and ACL mRNA level as well as acetyl CoA amount in the fungal cells were reduced to the levels observed in a trichothecene trace-production medium containing glucose or fructose. The ACL mRNA level was greatly increased by addition of cobalt chloride in the trichothecene high-production medium, but not in the trichothecene trace-production medium. Levels were reduced to those level in the trichothecene trace-production medium by addition of precocene II (300 µM) together with cobalt chloride. These results suggest that ACL expression is activated in the presence of sucrose and that acetyl CoA produced by the increased ALC level may be used for trichothecene production in the fungus. These findings also suggest that sucrose is important for the action of cobalt chloride in activating trichothecene production and that precocene II may affect a step down-stream of the target of cobalt chloride.

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Inhibitory activity of blasticidin A, a strong aflatoxin production inhibitor, on protein synthesis of yeast: selective inhibition of aflatoxin production by protein synthesis inhibitors

Cited by 38 publications

References 23 publications

Inhibitory Effects of Respiration Inhibitors on Aflatoxin Production

Inhibitory Effects of Respiration Inhibitors on Aflatoxin Production

Aflatoxins: Mechanisms of Inhibition by Antagonistic Plants and Microorganisms

Correlation of ATP Citrate Lyase and Acetyl CoA Levels with Trichothecene Production in Fusarium graminearum

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