Inhibitory Activity of Allium Sativum L. Extract Against Streptococcus Pyogenes and Pseudomonas Aeruginosa

Abstract

Background: One of the most common health problems is infectious diseases. Infectious disease can be caused by bacteria. There were two groups of bacteria based on the staining, Gram-positive and Gram-negative bacteria. Purpose: Antibiotics are the main therapy used in the incidence of bacterial infections. But over time, some antibiotics became resistance. Several studies have shown that garlic has an antibacterial effect. The content of allicin, ajoene, saponins, and flavonoids is found in garlic which has antibacterial properties. The antibiotic activity test of garlic was carried out on the bacteria Streptococcus pyogenes and Pseudomonas aeruginosa. The goal of this study is to investigate the antibacterial effect of Allium sativum L. extract against Streptococcus pyogenes and Pseudomonas aeruginosa. Methods: Garlic extract was made using the maceration method using 96% alcohol as the solvent. Tube dilution method elected to observe garlic antibiotic activity. This test aims to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). There were eight different concentration used, i.e. 2 grams/ml, 1 gram/ml, 0.5 gram/ml, 0.25 gram/ml, 0.125 gram/ml, 0.0625 gram/ml, 0.03125 gram/ml, and 0.015625 gram/ml. Replication is done three times. Results: In this experiment, the extract produced was turbid that MIC could not be determined and there was no momentous differentiation between before and after treatment. There was no growth of Streptococcus pyogenes in 1 gram/ml and Pseudomonas aeruginosa in 0.5 gram/ml. This number indicates the MBC for each bacteria. Conclusion: Garlic (Allium sativum L.) has an effect of bactericidal activity, it can perform as an antibacterial for Streptococcus pyogenes and Pseudomonas aeruginosa. Garlic extract was more effective for Pseudomonas aeruginosa than Streptococcus pyogenes.

Safety and Efficacy of Medicinal Plants Used to Manufacture Herbal Products with Regulatory Approval in Uganda: A Cross-Sectional Study

“…An ethanolic garlic extract was bactericidal against S. pyogenes , which is commonly implicated in bacterial URTI, with an MBC of 1 g/ml [ 159 ]. The antiviral activity of garlic has been demonstrated in the following viruses known to cause URTI in humans; adenovirus [ 160 ]; influenza A and influenza B virus [ 161 , 162 ]; human rhinovirus-2 [ 163 ]; and measles virus, Newcastle disease virus, and parainfluenza virus-3 [ 164 ].…”

Analysis of plasmid profiles of Escherichia coli bacteria and their resistance to several antibiotics

“…Plasmids can be seen when the genes they contain give new traits to the host such as resistance plasmids, virulence plasmids, degradative plasmids, sex plasmids, and col-plasmids (Wibowo, 2011). Antibiotics are drugs used to treat bacterial infections (Savitri et al, 2019). This antibiotic is a material secreted by microorganisms and has an antagonistic attitude toward the growth and life of other organisms.…”

Potensi Bawang Merah (Allium cepa L) dan Bawang Merah (Allium cepa L. var. aggregatum) Sebagai Daya Hambat Bakteri Pseudomonas aeruginosa

“…Salah satu masalah kesehatan yang terjadi pada Negara berkembang adalah penyakit infeksi yang di akibatkan oleh bakteri (Savitri et al, 2019). Bakteri Pseudomonas aeruginosa menyebabkan Infeksi Saluran Kemih (ISK).…”