Inhibitory Action of Quercetin on Eosinophil ActivationIn Vitro

Sakai-Kashiwabara, Misako; Asano, Koichiro

doi:10.1155/2013/127105

Cited by 28 publications

(23 citation statements)

References 33 publications

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“…Ekstrak daun jambu biji kaya akan flavonoid, terutama turunan quercetin 4 . Quercetin mampu menekan aktivasi sel mast atau memiliki efek mast cell stabilizer 5 . Quercetin dalam daun jambu biji (Psidium guajava) berpotensi sebagai antihistamin yang dapat menjadi terapi alternatif pada penyakit alergi melalui mekanisme mast cell stabilizer dan penurunan jumlah sitokin yang berperan dalam reaksi alergi 5 .…”

Section: Pendahuluanunclassified

“…Quercetin mampu menekan aktivasi sel mast atau memiliki efek mast cell stabilizer 5 . Quercetin dalam daun jambu biji (Psidium guajava) berpotensi sebagai antihistamin yang dapat menjadi terapi alternatif pada penyakit alergi melalui mekanisme mast cell stabilizer dan penurunan jumlah sitokin yang berperan dalam reaksi alergi 5 . Penelitian ini bertujuan untuk mengetahui efek antihistamin ekstrak daun jambu biji (Psidium guajava) pada tikus putih (Rattus novergicus) yang diinduksi ovalbumin dinilai dari jumlah eosinofil jaringan kulit.…”

Section: Pendahuluanunclassified

“…Melalui mekanisme tersebut, quercetin mampu menekan jumlah sel Th2 yang berperan penting dalam aktivasi dan rekrutmen eosinofil. Selain itu, quercetin berperan dalam menurunkan jumlah infiltrasi eosinofil pada jaringan melalui penekanan aktivasi sel mast (mast cell stabilizer) 5 . Hasil penelitian Kim et al (2014) menunjukkan quercetin secara signifikan mampu menekan ekspresi TNF-α dan IL-4 yang memiliki peranan penting dalam memicu dan mempertahankan reaksi alergi sel mast 10 .…”

Section: Pendahuluanunclassified

“…Hasil penelitian Kim et al (2014) menunjukkan quercetin secara signifikan mampu menekan ekspresi TNF-α dan IL-4 yang memiliki peranan penting dalam memicu dan mempertahankan reaksi alergi sel mast 10 . Selain melalui penekanan sel mast, quercetin mampu menurunkan sekresi sitokin dan ekpresi mRNA yang berperan dalam produksi, maturasi, dan rekrutmen eosinofil 5 . Pemberian quercetin pada hewan coba model asma juga memperlihatkan penurunan IL-4, IL-5, eotaksin CCL11 yang merupakan sitokin yang berperan dalam perkembangan, maturasi, dan rekrutmen eosinofil menuju tempat inflamasi 5,8,11 .…”

Section: Pendahuluanunclassified

“…Selain melalui penekanan sel mast, quercetin mampu menurunkan sekresi sitokin dan ekpresi mRNA yang berperan dalam produksi, maturasi, dan rekrutmen eosinofil 5 . Pemberian quercetin pada hewan coba model asma juga memperlihatkan penurunan IL-4, IL-5, eotaksin CCL11 yang merupakan sitokin yang berperan dalam perkembangan, maturasi, dan rekrutmen eosinofil menuju tempat inflamasi 5,8,11 . Sehingga berdasarkan mekanisme tersebut, quercetin mampu menurunkan jumlah infiltrasi eosinofil pada jaringan.…”

Section: Pendahuluanunclassified

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Efek Anti Histamin Ekstrak Daun Jambu Biji Pada Tikus Putih Yang Diinduksi Ovalbumin Dinilai Dari Jumlah Eosinofil Jaringan Kulit

2020

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ABSTRAKPendahuluan: Prevalensi dan keparahan penyakit alergi terus meningkat secara global, terutama di negara dengan pendapatan rendah sampai menengah. Terapi alergi saat ini seperti kortikosteroid banyak memberikan efek samping dalam penggunaan jangka panjang. Ekstrak daun jambu biji (Psidium guajava) berpotensi sebagai antihistamin yang dapat menjadi terapi alternatif penyakit alergi. Penelitian ini bertujuan untuk mengetahui efek antihistamin ekstrak daun jambu biji (Psidium guajava) pada tikus putih (Rattus novergicus) yang diinduksi ovalbumin dinilai dari jumlah eosinofil jaringan kulit. Metode: Penelitian ini merupakan penelitian quasi experimental design dengan rancangan post test only with control group design. Subjek penelitian tikus putih jantan, umur 3-4 bulan, dan berat badan 200-300 gram. Sampel 24 ekor tikus putih dibagi secara random menjadi enam kelompok. Kelompok kontrol normal (K) tanpa perlakuan, kontrol negatif (KN) hanya diinduksi ovalbumin, kontrol positif (KP) diberikan metilprednisolon, dan kelompok perlakuan (KP1, KP2, KP3) diberikan ekstrak daun jambu biji dengan dosis berikut 27 mg/200 gramBB, 54 mg/200 gramBB, dan 108 mg/200 gramBB. Efek antihistamin dinilai melalui gambaran histopatologi jaringan kulit berupa rata-rata jumlah infiltrasi eosinofil dengan menggunakan mikroskop cahaya. Data dianalisis menggunakan uji Kruskal Wallis dan dilanjutkan uji Post Hoc Mann Whitney (α= 0,05).Hasil: Uji Kruskal Wallis menunjukkan perbedaan signifikan di antara enam kelompok perlakuan (p=0,00). Uji Post Hoc Mann Whitney menunjukkan perbedaan signifikan di antara semua pasangan kelompok (p=<0,05). KP3 menunjukkan rerata jumlah eosinofil lebih rendah daripada KP dan signifikan (p=0,021). Kesimpulan: Ekstrak daun jambu biji (Psidium guajava) memiliki efek antihistamin dengan menurunkan infiltrasi eosinofil pada jaringan kulit tikus putih (Rattus norvegicus) yang dinduksi ovalbumin.Kata Kunci: Ekstrak daun jambu biji, eosinofil, tikus putih, histopatologi kulit. ABSTRACTIntroduction: The prevalence and severity of allergic diseases continues to increase globally, especially in countries with low to middle income. Current allergic therapies such as corticosteroids have many side effects in long-term use. Guava leaf extract (Psidium guajava) has the potential as an antihistamine which can be an alternative therapy for allergic diseases. This study aims to determine the effect of antihistamine extract of guava leaves (Psidium guajava) on white rats (Rattus novergicus) induced by ovalbumin assessed from the number of eosinophils of skin tissue. Methods: This research was a quasi experimental design with a post test only design with control group design. The research subjects were male white rats, aged 3-4 months, and weighing 200-300 grams. Samples of 24 white rats were randomly divided into six groups. Normal (K) control group without treatment, negative control (KN) was only induced by ovalbumin, positive control (KP) was given methylprednisolone, and treatment groups (KP1, KP2, KP3) were given guava leaf extract with the following dosage 27 mg / 200 gram BW, 54 mg / 200 gram BW, and 108 mg / 200 gram BW. The effect of antihistamines was assessed through histopathology of skin tissue in the form of an average number of eosinophil infiltrations using a light microscope. Data were analyzed using the Kruskal Wallis test and followed by the Mann Whitney Post Hoc test (α = 0.05).Result: The Kruskal Wallis test showed significant differences between the six treatment groups (p = 0.00). The Mann Whitney Post Hoc test showed significant differences between all group pairs (p = <0.05). KP3 shows the average number of eosinophils lower than KP and significant (p = 0.021). Conclusion: Guava leaf extract (Psidium guajava) has an antihistamine effect by reducing eosinophil infiltration in ovalbumin-induced white rat (Rattus norvegicus) skin tissue.Keywords: Guava leaf extract; eosinophil; skin histopathology

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Efek Anti Histamin Ekstrak Daun Jambu Biji Pada Tikus Putih Yang Diinduksi Ovalbumin Dinilai Dari Jumlah Eosinofil Jaringan Kulit

2020

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Experimental and clinical studies on the effects of Portulaca oleracea L. and its constituents on respiratory, allergic, and immunologic disorders, a review

Khazdair

Saadat

Aslani

et al. 2021

Phytotherapy Research

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Various pharmacological effects for Portulaca oleracea were shown in previous studies. Therefore, the effects of P. oleracea and its derivatives on respiratory, allergic, and immunologic diseases according to update experimental and clinical studies are provided in this review article. PubMed/Medline, Scopus, and Google Scholar were searched using appropriate keywords until the end of December 2020. The effects of P. oleracea and its constituents such as quercetin and kaempferol on an animal model of asthma were shown. Portulaca oleracea and its constituents also showed therapeutic effects on chronic obstructive pulmonary disease and chronic bronchitis in both experimental and clinical studies. The possible bronchodilatory effect of P. oleracea and its ingredients was also reported. Portulaca oleracea and its constituents showed the preventive effect on lung cancer and a clinical study showed the effect of P. oleracea on patients with lung adenocarcinoma. In addition, a various constituents of P. oleracea including, quercetin and kaempferol showed therapeutic effects on lung infections. This review indicates the therapeutic effect of P. oleracea and its constituents on various lung and allergic disorders but more clinical studies are required to establish the clinical efficacy of this plant and its constituents on lung and allergic disorders.

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Effectiveness of quercetin in an experimental rat model of allergic rhinitis

Sağıt

Polat

Gürgen

et al. 2017

Eur Arch Otorhinolaryngol

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We aimed to investigate whether quercetin had a therapeutic effect in an experimental rat model of allergic rhinitis. The study was conducted with 35 rats, which were randomly assigned into 4 groups: group 1 (n = 5), sham group; group 2 (quercetin group, n = 10) received 80 mg/kg day quercetin; group 3 (steroid group, n = 10) received steroid (mometasone furoate); and group 4 (control group, n = 10), received ovalbumin alone. Rats were sensitized by administration of ovalbumin on alternate days over 14 days via an intraperitoneal route. On day 15, in addition to ovalbumin via an intranasal route, quercetin and steroid were given over 7 days to the corresponding groups. All rats were then sacrificed and nasal turbinates were evaluated histopathologically, and serum total IgE and ovalbumin (OVA)-specific IgE values were measured before and after treatment. A significant increase in OVA-specific IgE values was detected in all groups except sham group. A significant increase was detected in post-treatment total IgE levels in the control group, while no significant change was detected in the sham, quercetin, and intranasal steroid groups. On histopathological evaluation, it was observed that findings of allergic rhinitis were suppressed in the quercetin group when compared to the control group. In immunohistochemical evaluation, it was detected that COX-2 and VIP expressions were weaker in the quercetin group compared to the control group. Based on these findings, we conclude that quercetin was effective in allergic rhinitis induced by ovalbumin in rats both histopathologically and serologically.

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Inhibitory Action of Quercetin on Eosinophil ActivationIn Vitro

Cited by 28 publications

References 33 publications

Efek Anti Histamin Ekstrak Daun Jambu Biji Pada Tikus Putih Yang Diinduksi Ovalbumin Dinilai Dari Jumlah Eosinofil Jaringan Kulit

Efek Anti Histamin Ekstrak Daun Jambu Biji Pada Tikus Putih Yang Diinduksi Ovalbumin Dinilai Dari Jumlah Eosinofil Jaringan Kulit

Experimental and clinical studies on the effects of Portulaca oleracea L. and its constituents on respiratory, allergic, and immunologic disorders, a review

Effectiveness of quercetin in an experimental rat model of allergic rhinitis

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