Inhibitory Action of Novel Arginine Derivative on Catecholamine Secretion Evoked by Acetylcholine from Cultured Bovine Adrenal Chromaffin Cells

Cilostazol, an Inhibitor of Type 3 Phosphodiesterase, Stimulates Large-Conductance, Calcium-Activated Potassium Channels in Pituitary GH3Cells and Pheochromocytoma PC12 Cells

“…The increase in intracellular cAMP was found to enhance Ca 2ϩ influx through l-type Ca 2ϩ channels in pituitary tumor cells (33). A recent report also demonstrated that inhibitory action of cilostazol on catecholamine secretion could be due to its blockade of Ca 2ϩ movement in adrenal chromaffin cells (7). For these reasons, we further investigated whether cilostazol exerts any effect on I Ca,L present in these cells.…”

“…Note that no modification in single-channel conductance was found in the presence of cilostazol, although it could increase the channel open probability. secretion in adrenal chromaffin cells (7). In this regard, the effect of cilostazol in pheochromocytoma PC12 cells was further examined.…”

“…It could potentiate the increase of this current caused by the stimulation of adenosine receptors with adenosine (5). In addition, it has been reported to suppress acetylcholine-induced catecholamine secretion in bovine adrenal chromaffin cells (7). The latter findings appeared not to be related to an increase in intracellular cAMP.…”

Differential Effect of Phosphodiesterase-3 Inhibitors on Sympathetic Hyperinnervation in Healed Rat Infarcts

“…40 Third, cilostazol appears to possess an additional effect that does not require inhibition of PDEs. 42 Cilostazol may directly inhibit secretion of catecholamine through its blocking action on Ca 2+ movement in adrenal chromaffin cells. 42 tricular remodeling after MI in rats treated with either milrinone or cilostazol, cilostazol was associated with neutrality in the development of sympathetic reinnervation after MI; however, milrinone was associated with a worsening of the incidence of fatal ventricular arrhythmias.…”

“…42 Cilostazol may directly inhibit secretion of catecholamine through its blocking action on Ca 2+ movement in adrenal chromaffin cells. 42 tricular remodeling after MI in rats treated with either milrinone or cilostazol, cilostazol was associated with neutrality in the development of sympathetic reinnervation after MI; however, milrinone was associated with a worsening of the incidence of fatal ventricular arrhythmias. The neutral effect of cilostazol on arrhythmia was attributable to an increase in the adenosine level in the remote myocardium, which counterbalanced cAMPinduced arrhythmias.…”