Inhibitory action of a new lectin from Xerocomus chrysenteron on cell-substrate adhesion

Abstract

Lectins are carbohydrate-binding proteins which potentially link to cell surface glycoconjugates and affect cell proliferation. We investigated the effect of a new lectin from the mushroom Xerocomus chrysenteron (XCL) on cell proliferation using adherent and suspension cell lines. XCL caused a dose-dependent inhibition of proliferation of the adherent cell lines NIH-3T3 and HeLa. Several experiments suggest that disruption of cell-substrate adhesion is the main factor affecting cell growth inhibition. (i) No antiproliferative effect was observed on the SF9 cell line, which does not require to be attached to grow. (ii) XCL was shown to affect the adherence of cells following their suspension by trypsin treatment. (iii) XCL was localized on the cell surface where it would act as a coating agent. (iv) XCL induced morphological changes from well spread to rounded cells and disrupted the actin cytoskeleton. By contrast, flow cytometric analysis showed that XCL does not interfere with the cell cycle, and does not induce apoptosis. (Mol Cell Biochem 258: [49][50][51][52][53][54][55] 2004)

Entomotoxic and nematotoxic lectins and protease inhibitors from fungal fruiting bodies

“…As an example, XCL was endocytosed by various animal and human cell lines and caused inhibition of proliferation of some of these cell lines (Francis et al 2003;Marty-Detraves et al 2004). A second issue is the way the protein is applied to the crop plant.…”

Structural Basis for the Carbohydrate Recognition of the Sclerotium rolfsii Lectin

“…XCL has potent insecticidal activity, 20 and blocks the proliferation of several cell lines by disrupting the cell-substrate adhesion. 21 XCL is similar to ABL, which also expresses selective cytotoxicity against malignant epithelial cell lines by blocking the sequence-dependent nuclear protein import. 47,48 SRL shares with ABL and XCL similar carbohydrate specificities for N-acetyl galactosamine and the TF-antigen.…”

“…19 XCL also shows antiproliferative activity on different cell lines and interestingly exhibits potent insecticidal activity. 20,21 Lectins from Coprineus cinerea (CGL2) 22 and Agrocybe cylindracea (ACG) 23 share a common carbohydrate recognition domain with galectins, a large family of β-galactoside-binding animal lectins with a highly conserved carbohydrate recognition domain (CRD). CGL2 has unique sugar specificity and binds strongly to the blood group A tetrasaccharide GalNAcα1-3 [Fucα1-2]Galβ1-4Glc; 24 on the other hand, ACG recognizes sialoconjugates.…”

A New Lectin Family with Structure Similarity to Actinoporins Revealed by the Crystal Structure of Xerocomus chrysenteron Lectin XCL

“…This effect is coupled to a dramatic change of the organization of the actin cytoskeleton. 10 Following its binding to the cell surface, XCL is internalized via the clathrin-dependent pathway and the protein is rapidly delivered to late endosome/lysosome compartments. 11 Our studies on XCL indicated potent insecticidal activity towards two insect models.…”