Inhibitors of protein synthesis by ribosomes of the 80-S type

Yeast ribosomal sensitivity and resistance to the Amaryllidaceae alkaloids

“…Minimal inhibitory concentration was considered the amount of drug that under the standard conditions used ( . Peptide bond formation was studied in the puromycin and the fragment reaction assays as reported [3,11 ]. Dr A. Battersby (University Chemical Laboratory, Cambridge, England) provided us with haemanthamine and lycorine; Dr C. Fuganti (Instituto di Chimica, Milano, Italy) with haemanthamine, lycorine, narciclasine and pseudolycorine; Dr E. Furusawa (School of Medicine, University of Hawaii, Honolulu) with lycorine, pretazettine and pseudolycorine; Dr H. Irie (Faculty of Pharmaceutical Sciences, Kyoto University, Japan) with haemanthamine and tazettine; Dr F. Piozzi (Instituto di Chimica Organica, Facolt~i di Scienze, Universit~ di Palermo, Italy) with narciclasine and Dr W. C. Wildman (Department of Chemistry, Iowa State University, USA) with haemanthamine and tazettine.…”

Combined Activity of Minocycline and Amphotericin B In Vitro Against Medically Important Yeasts

“…Tetracycline has no useful activity against intact yeast cells, although it inhibits protein synthesis by cell-free yeast ribosomes (2). Kwan and co-workers noted that tetracycline, at a concentration of 100 ,tg/ml, acted synergistically with amphotericin B (AmB) against a strain of Saccharomyces cerevisiae in vitro (6), and tetracycline subsequently was shown to potentiate the effect of AmB in treating experimental coccidioidomycosis in mice (5).…”

Mechanism of resistance to the antibiotic trichothecin in the producing fungi

“…Washed ribosomes were resuspended in buffer 1 and stored at -70°C. Ribosomal subunits were prepared by sucrose gradient centrifugation of 80s ribosomes treated with puromycin at 18 "C. Ribosomes and SlOO fraction from S. cerevisiae were obtained as previously described (Battaner and Vazquez, 1971).…”

“…Poly(U)-directed polyphenylalanine synthesis was performed, as indicated previously (Battaner and Vazquez, 1971), in 50-pl samples containing 0.3 pM 80s ribosomes, 5 pl cell extract (S-100 fraction), 1.5 mg/ml tRNA, 0.2 mg/ ml polyuridylic acid, 30 mM ['Hlphenylalanine (120 c p d pmol), 1 mM GTP, 10 mM ATP, 2.5 mg/ml phosphoenolpyruvate and 20 mg/ml pyruvate kinase in 50 mM Tris/HCl, pH7.6, 15 mM MgC12, 90mM KCI, 5 mM 2-mercaptoethanol. After incubation at 30°C for 20 min, samples were precipitated with 10% trichloroacetic acid, boiled for 10 min and filtered through glass-fiber filters.…”