Inhibitors of glucagon inactivationEffect on glucagon-receptor interactions and glucagon-stimulated adenylate cyclase activity in liver cell membranes

Binding of human growth hormone to rat liver membranes: Lactogenic and somatotropic sites

“…Liver membranes ('microsomal fraction') were prepared by differential centrifugation of rat liver homogenates in 0.25 M sucrose solution [7]. Protein concentration was determined by the method of Lowry [8] using bovine serum albumin as a standard.…”

Effect of cold acclimation on glucagon receptors of rat white adipocytes.

“…The effect of bacitracin is possibly due to inhibition of glucagon degradation in the reaction tube. Glucagon is ready to be degraded by the enzymes such as protease and peptidase in the assay preparation, and bacitracin most efficiently suppresses the activities of these enzymes (DESBUQUOIS et al, 1974).…”

“…Since bacitracin, a peptide antibiotic, is known to be a potent inhibitor of glucagon degradation (DESBUQUOIS et al, 1974), it was used to inhibit glucagon inactivation during receptor assay at 1 mg/ml in the reaction tube.…”

Action of proteolytic enzymes on lipotropins and endorphins: Biosynthesis, biotransformation and fate

“…1.1 hr. Bacitracin, a bacterial substance with aminopeptidase inhibiting properties (Desbuquois et al, 1974), did not affect the proteolysis of [125I]-fl-endorphin, but it increased the half-life of [125I]-~-endorphin to 3.6 hr. These results indicated firstly that the NH2-terminus of fl-endorphin is markedly more resistant to proteolytic attack than y-endorphin and secondly that cleavage of fl-endorphin is not initiated by aminopeptidases, but by other types of peptidases presumably endopeptidases.…”

“…The inhibition of tyrosine release by bacitracin, an antibiotic peptidase inhibitor (Desbuquois et al, 1974), provided more evidence for the involvement of aminopeptidases (Patthy et al, 1977). Moreover it appeared that also the release of phenylalanine, leucine, isoleucine and valine was markedly inhibited by bacitracin which led the authors to suggest that also brain carboxypeptidases were inhibited by bacitracin.…”