Inhibitors in the functional dissection of the photosynthetic electron transport system

Trebst, Achim

doi:10.1007/s11120-007-9213-x

Cited by 108 publications

(99 citation statements)

References 96 publications

(90 reference statements)

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“…of the Q o site of the cytochrome b 6 f complex (Farineau et al, 1984;Trebst, 2007); 2,5-dichloro-1,4-benzoquinone (DCBQ), an artificial electron acceptor from Q B in PSII (Graan and Ort, 1986); and 2,6-dichlorophenolindophenol (DCPIP), a strong oxidizing reagent at the acceptor side of PSI (Izawa, 1980;Supplemental Fig. S2A).…”

Section: Light Wavelength Required To Maintain the Activities Of Pptcmentioning

confidence: 99%

Light and CO₂/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum

et al. 2015

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Our previous study showed that three CO 2 /cAMP-responsive elements (CCRE) CCRE1, CCRE2, and CCRE3 in the promoter of the chloroplastic b-carbonic anhydrase 1 gene in the marine diatom Phaeodactylum tricornutum (Pptca1) were critical for the cAMPmediated transcriptional response to ambient CO 2 concentration. Pptca1 was activated under CO 2 limitation, but the absence of light partially disabled this low-CO 2 -triggered transcriptional activation. This suppression effect disappeared when CCRE2 or two of three CCREs were replaced with a NotI restriction site, strongly suggesting that light signal cross-talks with CO 2 on the cAMP-signal transduction pathway that targets CCREs. The paralogous chloroplastic carbonic anhydrase gene, ptca2 was also CO 2 /cAMP-responsive. The upstream truncation assay of the ptca2 promoter (Pptca2) revealed a short sequence of 2367 to 2333 relative to the transcription-start site to be a critical regulatory region for the CO 2 and light responses. This core-regulatory region comprises one CCRE1 and two CCRE2 sequences. Further detailed analysis of Pptca2 clearly indicates that two CCRE2s are the cis-element governing the CO 2 /light response of Pptca2. The transcriptional activation of two Pptcas in CO 2 limitation was evident under illumination with a photosynthetically active light wavelength, and an artificial electron acceptor from the reduction side of PSI efficiently inhibited Pptcas activation, while neither inhibition of the linear electron transport from PSII to PSI nor inhibition of ATP synthesis showed an effect on the promoter activity, strongly suggesting a specific involvement of the redox level of the stromal side of the PSI in the CO 2 /light cross talk.

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Section: Light Wavelength Required To Maintain the Activities Of Pptcmentioning

confidence: 99%

Light and CO₂/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum

et al. 2015

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“…DCMU causes a rapid increase to maximum fluorescence (F m ), where all PSII reaction centres are closed and the plastoquinone pool fully oxidised (Trebst 2007). DCMU has no impact on the membrane potential of the thylakoid in darkness, yet completely inhibits the lightinduced membrane pH gradient.…”

Section: Inhibitors Of Linear Electron Transportmentioning

confidence: 99%

“…Like DCMU, DBMIB (2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone) is also an inhibitor of electron transport, however it blocks further along the electron transport chain near the Cytochrome b 6 f complex (Trebst 2007). DBMIB is thought to interfere at the Reiske ironsulfur centre (Trebst 2007), thus blocking photosynthetic electron flow through the Cytochrome b 6 f complex.…”

Section: Inhibitors Of Linear Electron Transportmentioning

confidence: 99%

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Fluorescence as a Tool to Understand Changes in Photosynthetic Electron Flow Regulation

Ralph

Wilhelm

Lavaud

et al. 2010

Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications

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“…Because these inhibitors compete with the native quinone molecules, they are able to block the electron transfer from Q A to Q B (16,17). A very large number of compounds inhibit PSII electron transport, but only very few substances bind to pbRC.…”

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confidence: 99%

Structural Basis of Cyanobacterial Photosystem II Inhibition by the Herbicide Terbutryn

Broser

Glöckner

Габдулхаков

et al. 2011

Journal of Biological Chemistry

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Herbicides that target photosystem II (PSII) compete with the native electron acceptor plastoquinone for binding at the Q B site in the D1 subunit and thus block the electron transfer from Q A to Q B . Here, we present the first crystal structure of PSII with a bound herbicide at a resolution of 3.2 Å . The crystallized PSII core complexes were isolated from the thermophilic cyanobacterium Thermosynechococcus elongatus. The used herbicide terbutryn is found to bind via at least two hydrogen bonds to the Q B site similar to photosynthetic reaction centers in anoxygenic purple bacteria. Herbicide binding to PSII is also discussed regarding the influence on the redox potential of Q A , which is known to affect photoinhibition. We further identified a second and novel chloride position close to the water-oxidizing complex and in the vicinity of the chloride ion reported earlier (Guskov, A., Kern, J., Gabdulkhakov, A., Broser, M., Zouni, A., and Saenger, W. (2009) Nat. Struct. Mol. Biol. 16, 334 -342). This discovery is discussed in the context of proton transfer to the lumen.The process of photosynthesis converts solar energy into biochemically amenable energy. A distinction is made between oxygenic and anoxygenic photosynthesis. In the latter sulfur compounds, hydrogen gas or organic materials serve as electron source. In contrast, oxygenic photosynthesis in higher plants, algae, and cyanobacteria uses water as an electron source and generates molecular oxygen, thereby maintaining the level of oxygen in the atmosphere. Water oxidation takes place at the large homodimeric protein-cofactor complex photosystem II (PSII), 7 a light-driven water:plastoquinone oxidoreductase harbored in the thylakoid membrane (1-3). The structure of the PSII core complex (PSIIcc) from the thermophilic cyanobacterium Thermosynechococcus elongatus is known from x-ray crystallographic studies at a current resolution of 2.9 Å (4, 5). The photochemical reaction center (RC) in PSII is of type II and structurally related to the RC of purple bacteria (pbRC) (6), which perform anoxygenic photosynthesis. The PSII-RC contains four chlorophyll a (Chla) molecules (P D1 , P D2 , Chl D1 , and Chl D2 ), two pheophytins (Pheo D1 and Pheo D2 ), and two plastoquinones (PQ) (Q A and Q B ) with a nonheme iron in between. These cofactors are embedded in a heterodimeric protein matrix formed by subunits D1 and D2 (systematic names: PsbA and PsbD, respectively) and are arranged in two pseudo-C2 symmetric branches with respect to a 2-fold rotation axis, which crosses the non-heme iron and is oriented normal to the membrane plane.Photons from sunlight are collected by antenna proteins of PSII, and the excitation energy is transferred to the RC, where it gives rise to the formation of the radical pair P D1 ⅐ϩ Pheo D1 ⅐Ϫ and subsequent electron transfer to the fixed single-electron transmitter Q A . The electron hole at P D1 ⅐ϩ is able to abstract electrons via the redox-active tyrosine Y Z (Tyr-161A) from the heteronuclear Mn 4 Ca cluster located at the lumenal (d...

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Inhibitors in the functional dissection of the photosynthetic electron transport system

Cited by 108 publications

References 96 publications

Light and CO₂/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum

Light and CO₂/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum

Fluorescence as a Tool to Understand Changes in Photosynthetic Electron Flow Regulation

Structural Basis of Cyanobacterial Photosystem II Inhibition by the Herbicide Terbutryn

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Inhibitors in the functional dissection of the photosynthetic electron transport system

Cited by 108 publications

References 96 publications

Light and CO2/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum

Light and CO2/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum

Fluorescence as a Tool to Understand Changes in Photosynthetic Electron Flow Regulation

Structural Basis of Cyanobacterial Photosystem II Inhibition by the Herbicide Terbutryn

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Product

Resources

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Light and CO₂/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum

Light and CO₂/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum